Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4

Abstract Stronger contractility and smaller bladder capacity are common symptoms in ketamine cystitis (KC). This study investigates the association between expression levels of transient receptor potential cation channel subfamily V (TRPV) proteins and the clinical characteristics of KC. Bladder tis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hsueh-Hui Yang, Jia-Fong Jhang, Yung-Hsiang Hsu, Yuan-Hong Jiang, Wei-Jun Zhai, Hann-Chorng Kuo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e35d1886e1d40398df9b10099ff3358
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1e35d1886e1d40398df9b10099ff3358
record_format	dspace
spelling	oai:doaj.org-article:1e35d1886e1d40398df9b10099ff33582021-12-02T13:34:46ZSmaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV410.1038/s41598-021-84734-42045-2322https://doaj.org/article/1e35d1886e1d40398df9b10099ff33582021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84734-4https://doaj.org/toc/2045-2322Abstract Stronger contractility and smaller bladder capacity are common symptoms in ketamine cystitis (KC). This study investigates the association between expression levels of transient receptor potential cation channel subfamily V (TRPV) proteins and the clinical characteristics of KC. Bladder tissues were obtained from 24 patients with KC and four asymptomatic control subjects. Video urodynamic parameters were obtained before surgical procedures. The TRPV proteins were investigated by immunoblotting, immunofluorescence staining, and immunohistochemistry. The Pearson test was used to associate the expression levels of TRPV proteins with clinical characteristics of KC. The expression level of TRPV1 and TRPV4 was significantly higher in the severe KC bladders than in mild KC or control bladders. The TRPV1 proteins were localized in all urothelial cell layers, and TRPV4 was located in the basal cells and lamina propria. The expression of TRPV1 was negatively associated with maximal bladder capacity (r = − 0.66, P = 0.01). The expression of TRPV4 was positively associated with the velocity of detrusor pressure rise to the maximum flow rate (r = 0.53, P = 0.01). These observations suggest smaller bladder capacity and stronger contractility in KC are associated with an elevated expression of TRPV1 and TRPV4, respectively.Hsueh-Hui YangJia-Fong JhangYung-Hsiang HsuYuan-Hong JiangWei-Jun ZhaiHann-Chorng KuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hsueh-Hui Yang Jia-Fong Jhang Yung-Hsiang Hsu Yuan-Hong Jiang Wei-Jun Zhai Hann-Chorng Kuo Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4
description	Abstract Stronger contractility and smaller bladder capacity are common symptoms in ketamine cystitis (KC). This study investigates the association between expression levels of transient receptor potential cation channel subfamily V (TRPV) proteins and the clinical characteristics of KC. Bladder tissues were obtained from 24 patients with KC and four asymptomatic control subjects. Video urodynamic parameters were obtained before surgical procedures. The TRPV proteins were investigated by immunoblotting, immunofluorescence staining, and immunohistochemistry. The Pearson test was used to associate the expression levels of TRPV proteins with clinical characteristics of KC. The expression level of TRPV1 and TRPV4 was significantly higher in the severe KC bladders than in mild KC or control bladders. The TRPV1 proteins were localized in all urothelial cell layers, and TRPV4 was located in the basal cells and lamina propria. The expression of TRPV1 was negatively associated with maximal bladder capacity (r = − 0.66, P = 0.01). The expression of TRPV4 was positively associated with the velocity of detrusor pressure rise to the maximum flow rate (r = 0.53, P = 0.01). These observations suggest smaller bladder capacity and stronger contractility in KC are associated with an elevated expression of TRPV1 and TRPV4, respectively.
format	article
author	Hsueh-Hui Yang Jia-Fong Jhang Yung-Hsiang Hsu Yuan-Hong Jiang Wei-Jun Zhai Hann-Chorng Kuo
author_facet	Hsueh-Hui Yang Jia-Fong Jhang Yung-Hsiang Hsu Yuan-Hong Jiang Wei-Jun Zhai Hann-Chorng Kuo
author_sort	Hsueh-Hui Yang
title	Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4
title_short	Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4
title_full	Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4
title_fullStr	Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4
title_full_unstemmed	Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4
title_sort	smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated trpv1 and trpv4
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1e35d1886e1d40398df9b10099ff3358
work_keys_str_mv	AT hsuehhuiyang smallerbladdercapacityandstrongerbladdercontractilityinpatientswithketaminecystitisareassociatedwithelevatedtrpv1andtrpv4 AT jiafongjhang smallerbladdercapacityandstrongerbladdercontractilityinpatientswithketaminecystitisareassociatedwithelevatedtrpv1andtrpv4 AT yunghsianghsu smallerbladdercapacityandstrongerbladdercontractilityinpatientswithketaminecystitisareassociatedwithelevatedtrpv1andtrpv4 AT yuanhongjiang smallerbladdercapacityandstrongerbladdercontractilityinpatientswithketaminecystitisareassociatedwithelevatedtrpv1andtrpv4 AT weijunzhai smallerbladdercapacityandstrongerbladdercontractilityinpatientswithketaminecystitisareassociatedwithelevatedtrpv1andtrpv4 AT hannchorngkuo smallerbladdercapacityandstrongerbladdercontractilityinpatientswithketaminecystitisareassociatedwithelevatedtrpv1andtrpv4
_version_	1718392721080582144

Smaller bladder capacity and stronger bladder contractility in patients with ketamine cystitis are associated with elevated TRPV1 and TRPV4

Ejemplares similares