Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium

Abstract Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference compe...

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Autores principales:	Roghaieh Ashrafi, Matthieu Bruneaux, Lotta-Riina Sundberg, Katja Pulkkinen, Tarmo Ketola
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e3ab35f9a2146e9bbd72bff3cb22d92
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spelling	oai:doaj.org-article:1e3ab35f9a2146e9bbd72bff3cb22d922021-12-02T11:52:24ZApplication of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium10.1038/s41598-017-01074-y2045-2322https://doaj.org/article/1e3ab35f9a2146e9bbd72bff3cb22d922017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01074-yhttps://doaj.org/toc/2045-2322Abstract Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to-head competition between mixed genotype co-cultures of a waterborne bacterial pathogen of fish, Flavobacterium columnare, at two different temperatures. We found that competition outcome in liquid cultures seemed to be well predicted by growth yield of isolated strains, but was mostly inconsistent with interference competition results measured in inhibition tests on solid agar, especially as no growth inhibition between strain pairs was detected at the higher temperature. These results suggest that, for a given temperature, the factors driving competition outcome differ between liquid and solid environments.Roghaieh AshrafiMatthieu BruneauxLotta-Riina SundbergKatja PulkkinenTarmo KetolaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roghaieh Ashrafi Matthieu Bruneaux Lotta-Riina Sundberg Katja Pulkkinen Tarmo Ketola Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
description	Abstract Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to-head competition between mixed genotype co-cultures of a waterborne bacterial pathogen of fish, Flavobacterium columnare, at two different temperatures. We found that competition outcome in liquid cultures seemed to be well predicted by growth yield of isolated strains, but was mostly inconsistent with interference competition results measured in inhibition tests on solid agar, especially as no growth inhibition between strain pairs was detected at the higher temperature. These results suggest that, for a given temperature, the factors driving competition outcome differ between liquid and solid environments.
format	article
author	Roghaieh Ashrafi Matthieu Bruneaux Lotta-Riina Sundberg Katja Pulkkinen Tarmo Ketola
author_facet	Roghaieh Ashrafi Matthieu Bruneaux Lotta-Riina Sundberg Katja Pulkkinen Tarmo Ketola
author_sort	Roghaieh Ashrafi
title	Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
title_short	Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
title_full	Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
title_fullStr	Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
title_full_unstemmed	Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium
title_sort	application of high resolution melting assay (hrm) to study temperature-dependent intraspecific competition in a pathogenic bacterium
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1e3ab35f9a2146e9bbd72bff3cb22d92
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Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium

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