Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.

<h4>Background</h4>Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesis of the injury. This study is aimed at unraveling colonic microbiota alteration pattern and identifying specific bacterial species that differ...

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Autores principales:	Fan Wang, Qiurong Li, Chenyang Wang, Chun Tang, Jieshou Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e3e6d54fe274c4a8abcf3773f74fb41
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spelling	oai:doaj.org-article:1e3e6d54fe274c4a8abcf3773f74fb412021-11-18T07:10:37ZDynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.1932-620310.1371/journal.pone.0042027https://doaj.org/article/1e3e6d54fe274c4a8abcf3773f74fb412012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22848694/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesis of the injury. This study is aimed at unraveling colonic microbiota alteration pattern and identifying specific bacterial species that differ significantly as well as observing colonic epithelium change in the same injury model during the reperfusion time course.<h4>Methodology/principal findings</h4>Denaturing gradient gel electrophoresis (DGGE) was used to monitor the colonic microbiota of control rats and experimental rats that underwent 0.5 hour ischemia and 1, 3, 6, 12, 24, and 72 hours following reperfusion respectively. The microbiota similarity, bacterial diversity and species that characterized the dysbiosis were estimated based on the DGGE profiles using a combination of statistical approaches. The interested bacterial species in the gel were cut and sequenced and were subsequently quantified and confirmed with real-time PCR. Meanwhile, the epithelial barrier was checked by microscopy and D-lactate analysis. Colonic flora changed early and differed significantly at 6 hours after reperfusion and then started to recover. The shifts were characterized by the increase of Escherichia coli and Prevotella oralis, and Lactobacilli proliferation together with epithelia healing.<h4>Conclusion/significance</h4>This study shows for the first time that intestinal ischemia-reperfusion results in colonic flora dysbiosis that follows epithelia damage, and identifies the bacterial species that contribute most.Fan WangQiurong LiChenyang WangChun TangJieshou LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e42027 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fan Wang Qiurong Li Chenyang Wang Chun Tang Jieshou Li Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
description	<h4>Background</h4>Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesis of the injury. This study is aimed at unraveling colonic microbiota alteration pattern and identifying specific bacterial species that differ significantly as well as observing colonic epithelium change in the same injury model during the reperfusion time course.<h4>Methodology/principal findings</h4>Denaturing gradient gel electrophoresis (DGGE) was used to monitor the colonic microbiota of control rats and experimental rats that underwent 0.5 hour ischemia and 1, 3, 6, 12, 24, and 72 hours following reperfusion respectively. The microbiota similarity, bacterial diversity and species that characterized the dysbiosis were estimated based on the DGGE profiles using a combination of statistical approaches. The interested bacterial species in the gel were cut and sequenced and were subsequently quantified and confirmed with real-time PCR. Meanwhile, the epithelial barrier was checked by microscopy and D-lactate analysis. Colonic flora changed early and differed significantly at 6 hours after reperfusion and then started to recover. The shifts were characterized by the increase of Escherichia coli and Prevotella oralis, and Lactobacilli proliferation together with epithelia healing.<h4>Conclusion/significance</h4>This study shows for the first time that intestinal ischemia-reperfusion results in colonic flora dysbiosis that follows epithelia damage, and identifies the bacterial species that contribute most.
format	article
author	Fan Wang Qiurong Li Chenyang Wang Chun Tang Jieshou Li
author_facet	Fan Wang Qiurong Li Chenyang Wang Chun Tang Jieshou Li
author_sort	Fan Wang
title	Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
title_short	Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
title_full	Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
title_fullStr	Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
title_full_unstemmed	Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
title_sort	dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1e3e6d54fe274c4a8abcf3773f74fb41
work_keys_str_mv	AT fanwang dynamicalterationofthecolonicmicrobiotainintestinalischemiareperfusioninjury AT qiurongli dynamicalterationofthecolonicmicrobiotainintestinalischemiareperfusioninjury AT chenyangwang dynamicalterationofthecolonicmicrobiotainintestinalischemiareperfusioninjury AT chuntang dynamicalterationofthecolonicmicrobiotainintestinalischemiareperfusioninjury AT jieshouli dynamicalterationofthecolonicmicrobiotainintestinalischemiareperfusioninjury
_version_	1718423839489130496

Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.

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