Revealing RCOR2 as a regulatory component of nuclear speckles

Abstract Background Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency...

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Autores principales:	Carlos Rivera, Daniel Verbel-Vergara, Duxan Arancibia, Anna Lappala, Marcela González, Fabián Guzmán, Gianluca Merello, Jeannie T. Lee, María Estela Andrés
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	CoREST CoREST2 RCOR2 Nuclear speckles Membrane-less organelles SRRM2 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/1e42156d4a4f407eafaaf66356ce3bbc
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spelling	oai:doaj.org-article:1e42156d4a4f407eafaaf66356ce3bbc2021-11-28T12:23:26ZRevealing RCOR2 as a regulatory component of nuclear speckles10.1186/s13072-021-00425-41756-8935https://doaj.org/article/1e42156d4a4f407eafaaf66356ce3bbc2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13072-021-00425-4https://doaj.org/toc/1756-8935Abstract Background Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency maintenance and central nervous system development. Results Using biochemical approaches and high-resolution microscopy, we reveal that RCOR2 is localized in nuclear speckles across multiple cell types, including neurons in the brain. RCOR2 forms complexes with nuclear speckle components such as SON, SRSF7, and SRRM2. When cells are exposed to chemical stress, RCOR2 behaves as a core component of the nuclear speckle and is stabilized by RNA. In turn, nuclear speckle morphology appears to depend on RCOR2. Specifically, RCOR2 knockdown results larger nuclear speckles, whereas overexpressing RCOR2 leads to smaller and rounder nuclear speckles. Conclusion Our study suggests that RCOR2 is a regulatory component of the nuclear speckle bodies, setting this co-repressor protein as a factor that controls nuclear speckles behavior.Carlos RiveraDaniel Verbel-VergaraDuxan ArancibiaAnna LappalaMarcela GonzálezFabián GuzmánGianluca MerelloJeannie T. LeeMaría Estela AndrésBMCarticleCoRESTCoREST2RCOR2Nuclear specklesMembrane-less organellesSRRM2GeneticsQH426-470ENEpigenetics & Chromatin, Vol 14, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CoREST CoREST2 RCOR2 Nuclear speckles Membrane-less organelles SRRM2 Genetics QH426-470
spellingShingle	CoREST CoREST2 RCOR2 Nuclear speckles Membrane-less organelles SRRM2 Genetics QH426-470 Carlos Rivera Daniel Verbel-Vergara Duxan Arancibia Anna Lappala Marcela González Fabián Guzmán Gianluca Merello Jeannie T. Lee María Estela Andrés Revealing RCOR2 as a regulatory component of nuclear speckles
description	Abstract Background Nuclear processes such as transcription and RNA maturation can be impacted by subnuclear compartmentalization in condensates and nuclear bodies. Here, we characterize the nature of nuclear granules formed by REST corepressor 2 (RCOR2), a nuclear protein essential for pluripotency maintenance and central nervous system development. Results Using biochemical approaches and high-resolution microscopy, we reveal that RCOR2 is localized in nuclear speckles across multiple cell types, including neurons in the brain. RCOR2 forms complexes with nuclear speckle components such as SON, SRSF7, and SRRM2. When cells are exposed to chemical stress, RCOR2 behaves as a core component of the nuclear speckle and is stabilized by RNA. In turn, nuclear speckle morphology appears to depend on RCOR2. Specifically, RCOR2 knockdown results larger nuclear speckles, whereas overexpressing RCOR2 leads to smaller and rounder nuclear speckles. Conclusion Our study suggests that RCOR2 is a regulatory component of the nuclear speckle bodies, setting this co-repressor protein as a factor that controls nuclear speckles behavior.
format	article
author	Carlos Rivera Daniel Verbel-Vergara Duxan Arancibia Anna Lappala Marcela González Fabián Guzmán Gianluca Merello Jeannie T. Lee María Estela Andrés
author_facet	Carlos Rivera Daniel Verbel-Vergara Duxan Arancibia Anna Lappala Marcela González Fabián Guzmán Gianluca Merello Jeannie T. Lee María Estela Andrés
author_sort	Carlos Rivera
title	Revealing RCOR2 as a regulatory component of nuclear speckles
title_short	Revealing RCOR2 as a regulatory component of nuclear speckles
title_full	Revealing RCOR2 as a regulatory component of nuclear speckles
title_fullStr	Revealing RCOR2 as a regulatory component of nuclear speckles
title_full_unstemmed	Revealing RCOR2 as a regulatory component of nuclear speckles
title_sort	revealing rcor2 as a regulatory component of nuclear speckles
publisher	BMC
publishDate	2021
url	https://doaj.org/article/1e42156d4a4f407eafaaf66356ce3bbc
work_keys_str_mv	AT carlosrivera revealingrcor2asaregulatorycomponentofnuclearspeckles AT danielverbelvergara revealingrcor2asaregulatorycomponentofnuclearspeckles AT duxanarancibia revealingrcor2asaregulatorycomponentofnuclearspeckles AT annalappala revealingrcor2asaregulatorycomponentofnuclearspeckles AT marcelagonzalez revealingrcor2asaregulatorycomponentofnuclearspeckles AT fabianguzman revealingrcor2asaregulatorycomponentofnuclearspeckles AT gianlucamerello revealingrcor2asaregulatorycomponentofnuclearspeckles AT jeannietlee revealingrcor2asaregulatorycomponentofnuclearspeckles AT mariaestelaandres revealingrcor2asaregulatorycomponentofnuclearspeckles
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Revealing RCOR2 as a regulatory component of nuclear speckles

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