Fast wide-volume functional imaging of engineered in vitro brain tissues

Abstract The need for in vitro models that mimic the human brain to replace animal testing and allow high-throughput screening has driven scientists to develop new tools that reproduce tissue-like features on a chip. Three-dimensional (3D) in vitro cultures are emerging as an unmatched platform that...

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Autores principales:	G. Palazzolo, M. Moroni, A. Soloperto, G. Aletti, G. Naldi, M. Vassalli, T. Nieus, F. Difato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e4232b71cf04bb7b705b29fb09e2ca1
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spelling	oai:doaj.org-article:1e4232b71cf04bb7b705b29fb09e2ca12021-12-02T15:06:18ZFast wide-volume functional imaging of engineered in vitro brain tissues10.1038/s41598-017-08979-82045-2322https://doaj.org/article/1e4232b71cf04bb7b705b29fb09e2ca12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08979-8https://doaj.org/toc/2045-2322Abstract The need for in vitro models that mimic the human brain to replace animal testing and allow high-throughput screening has driven scientists to develop new tools that reproduce tissue-like features on a chip. Three-dimensional (3D) in vitro cultures are emerging as an unmatched platform that preserves the complexity of cell-to-cell connections within a tissue, improves cell survival, and boosts neuronal differentiation. In this context, new and flexible imaging approaches are required to monitor the functional states of 3D networks. Herein, we propose an experimental model based on 3D neuronal networks in an alginate hydrogel, a tunable wide-volume imaging approach, and an efficient denoising algorithm to resolve, down to single cell resolution, the 3D activity of hundreds of neurons expressing the calcium sensor GCaMP6s. Furthermore, we implemented a 3D co-culture system mimicking the contiguous interfaces of distinct brain tissues such as the cortical-hippocampal interface. The analysis of the network activity of single and layered neuronal co-cultures revealed cell-type-specific activities and an organization of neuronal subpopulations that changed in the two culture configurations. Overall, our experimental platform represents a simple, powerful and cost-effective platform for developing and monitoring living 3D layered brain tissue on chip structures with high resolution and high throughput.G. PalazzoloM. MoroniA. SolopertoG. AlettiG. NaldiM. VassalliT. NieusF. DifatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-20 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q G. Palazzolo M. Moroni A. Soloperto G. Aletti G. Naldi M. Vassalli T. Nieus F. Difato Fast wide-volume functional imaging of engineered in vitro brain tissues
description	Abstract The need for in vitro models that mimic the human brain to replace animal testing and allow high-throughput screening has driven scientists to develop new tools that reproduce tissue-like features on a chip. Three-dimensional (3D) in vitro cultures are emerging as an unmatched platform that preserves the complexity of cell-to-cell connections within a tissue, improves cell survival, and boosts neuronal differentiation. In this context, new and flexible imaging approaches are required to monitor the functional states of 3D networks. Herein, we propose an experimental model based on 3D neuronal networks in an alginate hydrogel, a tunable wide-volume imaging approach, and an efficient denoising algorithm to resolve, down to single cell resolution, the 3D activity of hundreds of neurons expressing the calcium sensor GCaMP6s. Furthermore, we implemented a 3D co-culture system mimicking the contiguous interfaces of distinct brain tissues such as the cortical-hippocampal interface. The analysis of the network activity of single and layered neuronal co-cultures revealed cell-type-specific activities and an organization of neuronal subpopulations that changed in the two culture configurations. Overall, our experimental platform represents a simple, powerful and cost-effective platform for developing and monitoring living 3D layered brain tissue on chip structures with high resolution and high throughput.
format	article
author	G. Palazzolo M. Moroni A. Soloperto G. Aletti G. Naldi M. Vassalli T. Nieus F. Difato
author_facet	G. Palazzolo M. Moroni A. Soloperto G. Aletti G. Naldi M. Vassalli T. Nieus F. Difato
author_sort	G. Palazzolo
title	Fast wide-volume functional imaging of engineered in vitro brain tissues
title_short	Fast wide-volume functional imaging of engineered in vitro brain tissues
title_full	Fast wide-volume functional imaging of engineered in vitro brain tissues
title_fullStr	Fast wide-volume functional imaging of engineered in vitro brain tissues
title_full_unstemmed	Fast wide-volume functional imaging of engineered in vitro brain tissues
title_sort	fast wide-volume functional imaging of engineered in vitro brain tissues
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1e4232b71cf04bb7b705b29fb09e2ca1
work_keys_str_mv	AT gpalazzolo fastwidevolumefunctionalimagingofengineeredinvitrobraintissues AT mmoroni fastwidevolumefunctionalimagingofengineeredinvitrobraintissues AT asoloperto fastwidevolumefunctionalimagingofengineeredinvitrobraintissues AT galetti fastwidevolumefunctionalimagingofengineeredinvitrobraintissues AT gnaldi fastwidevolumefunctionalimagingofengineeredinvitrobraintissues AT mvassalli fastwidevolumefunctionalimagingofengineeredinvitrobraintissues AT tnieus fastwidevolumefunctionalimagingofengineeredinvitrobraintissues AT fdifato fastwidevolumefunctionalimagingofengineeredinvitrobraintissues
_version_	1718388499053281280

Fast wide-volume functional imaging of engineered in vitro brain tissues

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