Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification...

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Autores principales:	Jana Pekarkova, Imrich Gablech, Tatiana Fialova, Ondrej Bilek, Zdenka Fohlerova
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	parylene-C micropillars selenium nanoparticles biocompatibility antimicrobial Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1e457a07e9a54e70b30955058fba3f31
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spelling	oai:doaj.org-article:1e457a07e9a54e70b30955058fba3f312021-12-03T07:20:11ZModifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability2296-418510.3389/fbioe.2021.782799https://doaj.org/article/1e457a07e9a54e70b30955058fba3f312021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.782799/fullhttps://doaj.org/toc/2296-4185Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C–coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.Jana PekarkovaJana PekarkovaImrich GablechImrich GablechTatiana FialovaOndrej BilekZdenka FohlerovaZdenka FohlerovaZdenka FohlerovaFrontiers Media S.A.articleparylene-Cmicropillarsselenium nanoparticlesbiocompatibilityantimicrobialBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	parylene-C micropillars selenium nanoparticles biocompatibility antimicrobial Biotechnology TP248.13-248.65
spellingShingle	parylene-C micropillars selenium nanoparticles biocompatibility antimicrobial Biotechnology TP248.13-248.65 Jana Pekarkova Jana Pekarkova Imrich Gablech Imrich Gablech Tatiana Fialova Ondrej Bilek Zdenka Fohlerova Zdenka Fohlerova Zdenka Fohlerova Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability
description	Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C–coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.
format	article
author	Jana Pekarkova Jana Pekarkova Imrich Gablech Imrich Gablech Tatiana Fialova Ondrej Bilek Zdenka Fohlerova Zdenka Fohlerova Zdenka Fohlerova
author_facet	Jana Pekarkova Jana Pekarkova Imrich Gablech Imrich Gablech Tatiana Fialova Ondrej Bilek Zdenka Fohlerova Zdenka Fohlerova Zdenka Fohlerova
author_sort	Jana Pekarkova
title	Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability
title_short	Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability
title_full	Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability
title_fullStr	Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability
title_full_unstemmed	Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability
title_sort	modifications of parylene by microstructures and selenium nanoparticles: evaluation of bacterial and mesenchymal stem cell viability
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1e457a07e9a54e70b30955058fba3f31
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_version_	1718373797507104768

Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability

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