Lignin Syngas Bioconversion by Butyribacterium methylotrophicum: Advancing towards an Integrated Biorefinery

Hybrid bio-thermochemical based technologies have the potential to ensure greater feedstock flexibility for the production of bioenergy and bioproducts. This study focused on the bioconversion of syngas produced from low grade technical lignin to C2-/C4-...

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Autores principales:	Marta Pacheco, Filomena Pinto, Joana Ortigueira, Carla Silva, Francisco Gírio, Patrícia Moura
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carboxydotrophic acetogenic bacteria carbon fixation crude syngas acetic acid butyric acid Technology T
Acceso en línea:	https://doaj.org/article/1e4640b1d41c4fbea014217ffabe118e
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spelling	oai:doaj.org-article:1e4640b1d41c4fbea014217ffabe118e2021-11-11T15:55:03ZLignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery10.3390/en142171241996-1073https://doaj.org/article/1e4640b1d41c4fbea014217ffabe118e2021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1073/14/21/7124https://doaj.org/toc/1996-1073Hybrid bio-thermochemical based technologies have the potential to ensure greater feedstock flexibility for the production of bioenergy and bioproducts. This study focused on the bioconversion of syngas produced from low grade technical lignin to C<sub>2</sub>-/C<sub>4</sub>-carboxylic acids by <i>Butyribacterium methylotrophicum</i>. The effects of pH, medium supplementation and the use of crude syngas were analyzed. At pH 6.0, <i>B. methylotrophicum</i> consumed CO, CO<sub>2</sub> and H<sub>2</sub> simultaneously up to 87 mol% of carbon fixation, and the supplementation of the medium with acetate increased the production of butyrate by 6.3 times. In long-term bioreactor experiments, <i>B. methylotrophicum</i> produced 38.3 and 51.1 mM acetic acid and 0.7 and 2.0 mM butyric acid from synthetic and lignin syngas, respectively. Carbon fixation reached 83 and 88 mol%, respectively. The lignin syngas conversion rate decreased from 13.3 to 0.9 NmL/h throughout the assay. The appearance of a grayish pellet and cell aggregates after approximately 220 h was indicative of tar deposition. Nevertheless, the stressed cells remained metabolically active and maintained acetate and butyrate production from lignin syngas. The challenge that impurities represent in the bioconversion of crude syngas has a direct impact on syngas cleaning requirements and operation costs, supporting the pursuit for more robust and versatile acetogens.Marta PachecoFilomena PintoJoana OrtigueiraCarla SilvaFrancisco GírioPatrícia MouraMDPI AGarticlecarboxydotrophicacetogenic bacteriacarbon fixationcrude syngasacetic acidbutyric acidTechnologyTENEnergies, Vol 14, Iss 7124, p 7124 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carboxydotrophic acetogenic bacteria carbon fixation crude syngas acetic acid butyric acid Technology T
spellingShingle	carboxydotrophic acetogenic bacteria carbon fixation crude syngas acetic acid butyric acid Technology T Marta Pacheco Filomena Pinto Joana Ortigueira Carla Silva Francisco Gírio Patrícia Moura Lignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery
description	Hybrid bio-thermochemical based technologies have the potential to ensure greater feedstock flexibility for the production of bioenergy and bioproducts. This study focused on the bioconversion of syngas produced from low grade technical lignin to C<sub>2</sub>-/C<sub>4</sub>-carboxylic acids by <i>Butyribacterium methylotrophicum</i>. The effects of pH, medium supplementation and the use of crude syngas were analyzed. At pH 6.0, <i>B. methylotrophicum</i> consumed CO, CO<sub>2</sub> and H<sub>2</sub> simultaneously up to 87 mol% of carbon fixation, and the supplementation of the medium with acetate increased the production of butyrate by 6.3 times. In long-term bioreactor experiments, <i>B. methylotrophicum</i> produced 38.3 and 51.1 mM acetic acid and 0.7 and 2.0 mM butyric acid from synthetic and lignin syngas, respectively. Carbon fixation reached 83 and 88 mol%, respectively. The lignin syngas conversion rate decreased from 13.3 to 0.9 NmL/h throughout the assay. The appearance of a grayish pellet and cell aggregates after approximately 220 h was indicative of tar deposition. Nevertheless, the stressed cells remained metabolically active and maintained acetate and butyrate production from lignin syngas. The challenge that impurities represent in the bioconversion of crude syngas has a direct impact on syngas cleaning requirements and operation costs, supporting the pursuit for more robust and versatile acetogens.
format	article
author	Marta Pacheco Filomena Pinto Joana Ortigueira Carla Silva Francisco Gírio Patrícia Moura
author_facet	Marta Pacheco Filomena Pinto Joana Ortigueira Carla Silva Francisco Gírio Patrícia Moura
author_sort	Marta Pacheco
title	Lignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery
title_short	Lignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery
title_full	Lignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery
title_fullStr	Lignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery
title_full_unstemmed	Lignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery
title_sort	lignin syngas bioconversion by <i>butyribacterium methylotrophicum</i>: advancing towards an integrated biorefinery
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1e4640b1d41c4fbea014217ffabe118e
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Lignin Syngas Bioconversion by <i>Butyribacterium methylotrophicum</i>: Advancing towards an Integrated Biorefinery

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