Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel

Meiwan Chen1,2,&Dagger;, Zhiwen Yang1,&Dagger;, Hongmei Wu1, Xin Pan1, Xiaobao Xie3, Chuanbin Wu11Research and Development Center of Pharmaceutical Engineering, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China; 2State Key Laboratory of Quality Research in C...

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Autores principales:	Chen M, Yang Z, Wu H, Pan X, Xie X, Wu C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1e5010d8047d4289a0c0dce936fefc13
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spelling	oai:doaj.org-article:1e5010d8047d4289a0c0dce936fefc132021-12-02T05:00:35ZAntimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel1176-91141178-2013https://doaj.org/article/1e5010d8047d4289a0c0dce936fefc132011-11-01T00:00:00Zhttp://www.dovepress.com/antimicrobial-activity-and-the-mechanism-of-silver-nanoparticle-thermo-a8662https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Meiwan Chen1,2,&Dagger;, Zhiwen Yang1,&Dagger;, Hongmei Wu1, Xin Pan1, Xiaobao Xie3, Chuanbin Wu11Research and Development Center of Pharmaceutical Engineering, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China; 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; 3Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, China &Dagger;These authors contributed equally to this workPurpose: The purpose of the present study was to elucidate the antimicrobial activity and mechanism of silver nanoparticles incorporated into thermosensitive gel (S-T-Gel) on Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.Patients and methods: This study investigated the growth, permeability, and morphology of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa cells in order to observe the action of S-T-Gel on the membrane structure of these three bacteria. The cell morphology of normal and treated bacteria cells was assessed by transmission electron microscopy (TEM), and the effects of S-T-Gel on genome DNA of bacterial cells were evaluated by agarose gel electrophoresis.Results: S-T-Gel showed promising activity against Staphylococcus aureus and moderate activity against Escherichia coli and Pseudomonas aeruginosa. The observation with TEM suggested that S-T-Gel may destroy the structure of bacterial cell membranes in order to enter the bacterial cell. S-T-Gel then condensed DNA and combined and coagulated with the cytoplasm of the damaged bacteria, resulting in the leakage of the cytoplasmic component and the eventual death of these three bacteria. In addition, the analysis of agarose gel electrophoresis demonstrated that S-T-Gel could increase the decomposability of genome DNA.Conclusion: These results about promising antimicrobial activity and mechanism of S-T-Gel may be useful for further research and development in in-vivo studies.Keywords: molecule mechanism, bacterial cells, S-T-GelChen MYang ZWu HPan XXie XWu CDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2873-2877 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen M Yang Z Wu H Pan X Xie X Wu C Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel
description	Meiwan Chen1,2,&Dagger;, Zhiwen Yang1,&Dagger;, Hongmei Wu1, Xin Pan1, Xiaobao Xie3, Chuanbin Wu11Research and Development Center of Pharmaceutical Engineering, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China; 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China; 3Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, China &Dagger;These authors contributed equally to this workPurpose: The purpose of the present study was to elucidate the antimicrobial activity and mechanism of silver nanoparticles incorporated into thermosensitive gel (S-T-Gel) on Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.Patients and methods: This study investigated the growth, permeability, and morphology of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa cells in order to observe the action of S-T-Gel on the membrane structure of these three bacteria. The cell morphology of normal and treated bacteria cells was assessed by transmission electron microscopy (TEM), and the effects of S-T-Gel on genome DNA of bacterial cells were evaluated by agarose gel electrophoresis.Results: S-T-Gel showed promising activity against Staphylococcus aureus and moderate activity against Escherichia coli and Pseudomonas aeruginosa. The observation with TEM suggested that S-T-Gel may destroy the structure of bacterial cell membranes in order to enter the bacterial cell. S-T-Gel then condensed DNA and combined and coagulated with the cytoplasm of the damaged bacteria, resulting in the leakage of the cytoplasmic component and the eventual death of these three bacteria. In addition, the analysis of agarose gel electrophoresis demonstrated that S-T-Gel could increase the decomposability of genome DNA.Conclusion: These results about promising antimicrobial activity and mechanism of S-T-Gel may be useful for further research and development in in-vivo studies.Keywords: molecule mechanism, bacterial cells, S-T-Gel
format	article
author	Chen M Yang Z Wu H Pan X Xie X Wu C
author_facet	Chen M Yang Z Wu H Pan X Xie X Wu C
author_sort	Chen M
title	Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel
title_short	Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel
title_full	Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel
title_fullStr	Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel
title_full_unstemmed	Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel
title_sort	antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/1e5010d8047d4289a0c0dce936fefc13
work_keys_str_mv	AT chenm antimicrobialactivityandthemechanismofsilvernanoparticlethermosensitivegel AT yangz antimicrobialactivityandthemechanismofsilvernanoparticlethermosensitivegel AT wuh antimicrobialactivityandthemechanismofsilvernanoparticlethermosensitivegel AT panx antimicrobialactivityandthemechanismofsilvernanoparticlethermosensitivegel AT xiex antimicrobialactivityandthemechanismofsilvernanoparticlethermosensitivegel AT wuc antimicrobialactivityandthemechanismofsilvernanoparticlethermosensitivegel
_version_	1718400816470032384

Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel

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