Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis

A new cell line was isolated and characterized from the head kidney of Siganus fuscescens (rabbit fish). The new macrophagic-like cell line was named as rabbit fish macrophage (RFM), and which could be sub-cultured for over 50 cycles since the development. RFM cell line was tested for growth in diff...

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Autores principales:	Yi-Teng Xia, Edwin Hok-Chi Cheng, Ying-Jie Xia, Qi-Yun Wu, Lynn Hoi-Lam Zhang, Sheng-Ying Lin, Tina Ting-Xia Dong, Qi-Wei Qin, Wen-Xiong Wang, Karl Wah-Keung Tsim
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell line establishment Siganus fuscescens Anti-inflammation Traditional Chinese medicine Scutellaria baicalensis Zoology QL1-991
Acceso en línea:	https://doaj.org/article/1e50b874fce2422699acccda64093cd9
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spelling	oai:doaj.org-article:1e50b874fce2422699acccda64093cd92021-11-20T05:15:49ZCharacterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis2667-011910.1016/j.fsirep.2021.100036https://doaj.org/article/1e50b874fce2422699acccda64093cd92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667011921000311https://doaj.org/toc/2667-0119A new cell line was isolated and characterized from the head kidney of Siganus fuscescens (rabbit fish). The new macrophagic-like cell line was named as rabbit fish macrophage (RFM), and which could be sub-cultured for over 50 cycles since the development. RFM cell line was tested for growth in different temperatures and serum concentrations: the best growing condition was optimized at 20% serum under 28 °C. In cultured RFM cells, sequencing of 18S rRNA, as well as immunostaining of cytokeratin and CD 68, confirmed the identity as macrophagic cell of S. fuscescens. Cultured RFM cells were exposed to challenge of inflammation, as triggered by LPS, showing highly sensitive responses to inflammation, including release of nitric oxide, expression of cytokine, and activation of phagocytosis. The water extract of aerial part of Scutellaria baicalensis, named as SBA, has been shown anti-inflammatory property in S. fuscescens fish. In order to extend the application of SBA in aquaculture, the extract and its effective flavonoids, i.e. baicalin and scutellarin, were applied in LPS-treated RFM cells. Application of SBA extract, baicalin or scutellarin, inhibited the expressions of LPS-induced inflammatory cytokines, i.e. IL-1β, TNF-α, as well as the signaling of transcription factor NF-κB. The results support the established RFM cell line could be an ideal in vitro model in drug screening relating to inflammation. Additionally, the notion of SBA herbal extract in fish aquaculture is supported by its efficacy against inflammation.Yi-Teng XiaEdwin Hok-Chi ChengYing-Jie XiaQi-Yun WuLynn Hoi-Lam ZhangSheng-Ying LinTina Ting-Xia DongQi-Wei QinWen-Xiong WangKarl Wah-Keung TsimElsevierarticleCell line establishmentSiganus fuscescensAnti-inflammationTraditional Chinese medicineScutellaria baicalensisZoologyQL1-991ENFish and Shellfish Immunology Reports, Vol 2, Iss , Pp 100036- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell line establishment Siganus fuscescens Anti-inflammation Traditional Chinese medicine Scutellaria baicalensis Zoology QL1-991
spellingShingle	Cell line establishment Siganus fuscescens Anti-inflammation Traditional Chinese medicine Scutellaria baicalensis Zoology QL1-991 Yi-Teng Xia Edwin Hok-Chi Cheng Ying-Jie Xia Qi-Yun Wu Lynn Hoi-Lam Zhang Sheng-Ying Lin Tina Ting-Xia Dong Qi-Wei Qin Wen-Xiong Wang Karl Wah-Keung Tsim Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
description	A new cell line was isolated and characterized from the head kidney of Siganus fuscescens (rabbit fish). The new macrophagic-like cell line was named as rabbit fish macrophage (RFM), and which could be sub-cultured for over 50 cycles since the development. RFM cell line was tested for growth in different temperatures and serum concentrations: the best growing condition was optimized at 20% serum under 28 °C. In cultured RFM cells, sequencing of 18S rRNA, as well as immunostaining of cytokeratin and CD 68, confirmed the identity as macrophagic cell of S. fuscescens. Cultured RFM cells were exposed to challenge of inflammation, as triggered by LPS, showing highly sensitive responses to inflammation, including release of nitric oxide, expression of cytokine, and activation of phagocytosis. The water extract of aerial part of Scutellaria baicalensis, named as SBA, has been shown anti-inflammatory property in S. fuscescens fish. In order to extend the application of SBA in aquaculture, the extract and its effective flavonoids, i.e. baicalin and scutellarin, were applied in LPS-treated RFM cells. Application of SBA extract, baicalin or scutellarin, inhibited the expressions of LPS-induced inflammatory cytokines, i.e. IL-1β, TNF-α, as well as the signaling of transcription factor NF-κB. The results support the established RFM cell line could be an ideal in vitro model in drug screening relating to inflammation. Additionally, the notion of SBA herbal extract in fish aquaculture is supported by its efficacy against inflammation.
format	article
author	Yi-Teng Xia Edwin Hok-Chi Cheng Ying-Jie Xia Qi-Yun Wu Lynn Hoi-Lam Zhang Sheng-Ying Lin Tina Ting-Xia Dong Qi-Wei Qin Wen-Xiong Wang Karl Wah-Keung Tsim
author_facet	Yi-Teng Xia Edwin Hok-Chi Cheng Ying-Jie Xia Qi-Yun Wu Lynn Hoi-Lam Zhang Sheng-Ying Lin Tina Ting-Xia Dong Qi-Wei Qin Wen-Xiong Wang Karl Wah-Keung Tsim
author_sort	Yi-Teng Xia
title	Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
title_short	Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
title_full	Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
title_fullStr	Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
title_full_unstemmed	Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis
title_sort	characterization of a macrophagic-like cell line derived from rabbit fish (siganus fuscescens): an illustration of anti-inflammatory responses of the herbal extract of scutellaria baicalensis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/1e50b874fce2422699acccda64093cd9
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Characterization of a macrophagic-like cell line derived from rabbit fish (Siganus fuscescens): An illustration of anti-inflammatory responses of the herbal extract of Scutellaria baicalensis

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