HDL-apoA-I exchange: rapid detection and association with atherosclerosis.
High density lipoprotein (HDL) cholesterol levels are associated with decreased risk of cardiovascular disease, but not all HDL are functionally equivalent. A primary determinant of HDL functional status is the conformational adaptability of its main protein component, apoA-I, an exchangeable apolip...
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2013
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oai:doaj.org-article:1e58836a5d004ecdb31766e8e2e6b96a2021-11-18T08:57:57ZHDL-apoA-I exchange: rapid detection and association with atherosclerosis.1932-620310.1371/journal.pone.0071541https://doaj.org/article/1e58836a5d004ecdb31766e8e2e6b96a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24015188/?tool=EBIhttps://doaj.org/toc/1932-6203High density lipoprotein (HDL) cholesterol levels are associated with decreased risk of cardiovascular disease, but not all HDL are functionally equivalent. A primary determinant of HDL functional status is the conformational adaptability of its main protein component, apoA-I, an exchangeable apolipoprotein. Chemical modification of apoA-I, as may occur under conditions of inflammation or diabetes, can severely impair HDL function and is associated with the presence of cardiovascular disease. Chemical modification of apoA-I also impairs its ability to exchange on and off HDL, a critical process in reverse cholesterol transport. In this study, we developed a method using electron paramagnetic resonance spectroscopy (EPR) to quantify HDL-apoA-I exchange. Using this approach, we measured the degree of HDL-apoA-I exchange for HDL isolated from rabbits fed a high fat, high cholesterol diet, as well as human subjects with acute coronary syndrome and metabolic syndrome. We observed that HDL-apoA-I exchange was markedly reduced when atherosclerosis was present, or when the subject carries at least one risk factor of cardiovascular disease. These results show that HDL-apoA-I exchange is a clinically relevant measure of HDL function pertinent to cardiovascular disease.Mark S BorjaLei ZhaoBradley HammersonChongren TangRichard YangNancy CarsonGayani FernandoXiaoqin LiuMadhu S BudamaguntaJacques GenestGregory C ShearerFranck DuclosMichael N OdaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71541 (2013) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Mark S Borja Lei Zhao Bradley Hammerson Chongren Tang Richard Yang Nancy Carson Gayani Fernando Xiaoqin Liu Madhu S Budamagunta Jacques Genest Gregory C Shearer Franck Duclos Michael N Oda HDL-apoA-I exchange: rapid detection and association with atherosclerosis. |
| description |
High density lipoprotein (HDL) cholesterol levels are associated with decreased risk of cardiovascular disease, but not all HDL are functionally equivalent. A primary determinant of HDL functional status is the conformational adaptability of its main protein component, apoA-I, an exchangeable apolipoprotein. Chemical modification of apoA-I, as may occur under conditions of inflammation or diabetes, can severely impair HDL function and is associated with the presence of cardiovascular disease. Chemical modification of apoA-I also impairs its ability to exchange on and off HDL, a critical process in reverse cholesterol transport. In this study, we developed a method using electron paramagnetic resonance spectroscopy (EPR) to quantify HDL-apoA-I exchange. Using this approach, we measured the degree of HDL-apoA-I exchange for HDL isolated from rabbits fed a high fat, high cholesterol diet, as well as human subjects with acute coronary syndrome and metabolic syndrome. We observed that HDL-apoA-I exchange was markedly reduced when atherosclerosis was present, or when the subject carries at least one risk factor of cardiovascular disease. These results show that HDL-apoA-I exchange is a clinically relevant measure of HDL function pertinent to cardiovascular disease. |
| format |
article |
| author |
Mark S Borja Lei Zhao Bradley Hammerson Chongren Tang Richard Yang Nancy Carson Gayani Fernando Xiaoqin Liu Madhu S Budamagunta Jacques Genest Gregory C Shearer Franck Duclos Michael N Oda |
| author_facet |
Mark S Borja Lei Zhao Bradley Hammerson Chongren Tang Richard Yang Nancy Carson Gayani Fernando Xiaoqin Liu Madhu S Budamagunta Jacques Genest Gregory C Shearer Franck Duclos Michael N Oda |
| author_sort |
Mark S Borja |
| title |
HDL-apoA-I exchange: rapid detection and association with atherosclerosis. |
| title_short |
HDL-apoA-I exchange: rapid detection and association with atherosclerosis. |
| title_full |
HDL-apoA-I exchange: rapid detection and association with atherosclerosis. |
| title_fullStr |
HDL-apoA-I exchange: rapid detection and association with atherosclerosis. |
| title_full_unstemmed |
HDL-apoA-I exchange: rapid detection and association with atherosclerosis. |
| title_sort |
hdl-apoa-i exchange: rapid detection and association with atherosclerosis. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/1e58836a5d004ecdb31766e8e2e6b96a |
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