Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola

Abstract Aquaporins (AQPs) are of vital importance in the cellular transport system of all living organisms. In this study, genome-wide identification, distribution, and characterization of AQPs were determined in Arabidopsis lyrata, Capsella grandiflora, C. rubella, Eutrema salsugineum, Brassica ra...

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Autores principales:	Humira Sonah, Rupesh K. Deshmukh, Caroline Labbé, Richard R. Bélanger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e5a34f918614ebcb2aec0dd0852641c
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spelling	oai:doaj.org-article:1e5a34f918614ebcb2aec0dd0852641c2021-12-02T16:07:02ZAnalysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola10.1038/s41598-017-02877-92045-2322https://doaj.org/article/1e5a34f918614ebcb2aec0dd0852641c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02877-9https://doaj.org/toc/2045-2322Abstract Aquaporins (AQPs) are of vital importance in the cellular transport system of all living organisms. In this study, genome-wide identification, distribution, and characterization of AQPs were determined in Arabidopsis lyrata, Capsella grandiflora, C. rubella, Eutrema salsugineum, Brassica rapa, B. oleracea, and B. napus (canola). Classification and phylogeny of AQPs revealed the loss of XIPs and NIP-IIIs in all species. Characterization of distinctive AQP features showed a high level of conservation in spacing between NPA-domains, and selectivity filters. Interestingly, TIP3s were found to be highly expressed in developing seeds, suggesting their role in seed desiccation. Analysis of available RNA-seq data obtained under biotic and abiotic stresses led to the identification of AQPs involved in stress tolerance mechanisms in canola. In addition, analysis of the effect of ploidy level, and resulting gene dose effect performed with the different combinations of Brassica A and C genomes revealed that more than 70% of AQPs expression were dose-independent, thereby supporting their role in stress alleviation. This first in-depth characterization of Brassicaceae AQPs highlights transport mechanisms and related physiological processes that could be exploited in breeding programs of stress-tolerant cultivars.Humira SonahRupesh K. DeshmukhCaroline LabbéRichard R. BélangerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Humira Sonah Rupesh K. Deshmukh Caroline Labbé Richard R. Bélanger Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
description	Abstract Aquaporins (AQPs) are of vital importance in the cellular transport system of all living organisms. In this study, genome-wide identification, distribution, and characterization of AQPs were determined in Arabidopsis lyrata, Capsella grandiflora, C. rubella, Eutrema salsugineum, Brassica rapa, B. oleracea, and B. napus (canola). Classification and phylogeny of AQPs revealed the loss of XIPs and NIP-IIIs in all species. Characterization of distinctive AQP features showed a high level of conservation in spacing between NPA-domains, and selectivity filters. Interestingly, TIP3s were found to be highly expressed in developing seeds, suggesting their role in seed desiccation. Analysis of available RNA-seq data obtained under biotic and abiotic stresses led to the identification of AQPs involved in stress tolerance mechanisms in canola. In addition, analysis of the effect of ploidy level, and resulting gene dose effect performed with the different combinations of Brassica A and C genomes revealed that more than 70% of AQPs expression were dose-independent, thereby supporting their role in stress alleviation. This first in-depth characterization of Brassicaceae AQPs highlights transport mechanisms and related physiological processes that could be exploited in breeding programs of stress-tolerant cultivars.
format	article
author	Humira Sonah Rupesh K. Deshmukh Caroline Labbé Richard R. Bélanger
author_facet	Humira Sonah Rupesh K. Deshmukh Caroline Labbé Richard R. Bélanger
author_sort	Humira Sonah
title	Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
title_short	Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
title_full	Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
title_fullStr	Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
title_full_unstemmed	Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
title_sort	analysis of aquaporins in brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1e5a34f918614ebcb2aec0dd0852641c
work_keys_str_mv	AT humirasonah analysisofaquaporinsinbrassicaceaespeciesrevealshighlevelofconservationanddynamicroleagainstbioticandabioticstressincanola AT rupeshkdeshmukh analysisofaquaporinsinbrassicaceaespeciesrevealshighlevelofconservationanddynamicroleagainstbioticandabioticstressincanola AT carolinelabbe analysisofaquaporinsinbrassicaceaespeciesrevealshighlevelofconservationanddynamicroleagainstbioticandabioticstressincanola AT richardrbelanger analysisofaquaporinsinbrassicaceaespeciesrevealshighlevelofconservationanddynamicroleagainstbioticandabioticstressincanola
_version_	1718384764190195712

Analysis of aquaporins in Brassicaceae species reveals high-level of conservation and dynamic role against biotic and abiotic stress in canola

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