The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.

Regulation of the extracellular matrix (ECM) plays an important functional role either in physiological or pathological conditions. The plasminogen activation (PA) system, comprising the uPA and tPA proteases and their inhibitor PAI-1, is one of the main suppliers of extracellular proteolytic activi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ola Hadadeh, Emilie Barruet, Franck Peiretti, Monique Verdier, Denis Bernot, Yasmine Hadjal, Claire El Yazidi, Andrée Robaglia-Schlupp, Andre Maues De Paula, Didier Nègre, Michelina Iacovino, Michael Kyba, Marie-Christine Alessi, Bernard Binétruy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e5f7d84cb734b4dba74ed68d267a65e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1e5f7d84cb734b4dba74ed68d267a65e
record_format	dspace
spelling	oai:doaj.org-article:1e5f7d84cb734b4dba74ed68d267a65e2021-11-18T08:09:22ZThe plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.1932-620310.1371/journal.pone.0049065https://doaj.org/article/1e5f7d84cb734b4dba74ed68d267a65e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23145071/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Regulation of the extracellular matrix (ECM) plays an important functional role either in physiological or pathological conditions. The plasminogen activation (PA) system, comprising the uPA and tPA proteases and their inhibitor PAI-1, is one of the main suppliers of extracellular proteolytic activity contributing to tissue remodeling. Although its function in development is well documented, its precise role in mouse embryonic stem cell (ESC) differentiation in vitro is unknown. We found that the PA system components are expressed at very low levels in undifferentiated ESCs and that upon differentiation uPA activity is detected mainly transiently, whereas tPA activity and PAI-1 protein are maximum in well differentiated cells. Adipocyte formation by ESCs is inhibited by amiloride treatment, a specific uPA inhibitor. Likewise, ESCs expressing ectopic PAI-1 under the control of an inducible expression system display reduced adipogenic capacities after induction of the gene. Furthermore, the adipogenic differentiation capacities of PAI-1(-/-) induced pluripotent stem cells (iPSCs) are augmented as compared to wt iPSCs. Our results demonstrate that the control of ESC adipogenesis by the PA system correspond to different successive steps from undifferentiated to well differentiated ESCs. Similarly, skeletal myogenesis is decreased by uPA inhibition or PAI-1 overexpression during the terminal step of differentiation. However, interfering with uPA during days 0 to 3 of the differentiation process augments ESC myotube formation. Neither neurogenesis, cardiomyogenesis, endothelial cell nor smooth muscle formation are affected by amiloride or PAI-1 induction. Our results show that the PA system is capable to specifically modulate adipogenesis and skeletal myogenesis of ESCs by successive different molecular mechanisms.Ola HadadehEmilie BarruetFranck PeirettiMonique VerdierDenis BernotYasmine HadjalClaire El YazidiAndrée Robaglia-SchluppAndre Maues De PaulaDidier NègreMichelina IacovinoMichael KybaMarie-Christine AlessiBernard BinétruyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49065 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ola Hadadeh Emilie Barruet Franck Peiretti Monique Verdier Denis Bernot Yasmine Hadjal Claire El Yazidi Andrée Robaglia-Schlupp Andre Maues De Paula Didier Nègre Michelina Iacovino Michael Kyba Marie-Christine Alessi Bernard Binétruy The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.
description	Regulation of the extracellular matrix (ECM) plays an important functional role either in physiological or pathological conditions. The plasminogen activation (PA) system, comprising the uPA and tPA proteases and their inhibitor PAI-1, is one of the main suppliers of extracellular proteolytic activity contributing to tissue remodeling. Although its function in development is well documented, its precise role in mouse embryonic stem cell (ESC) differentiation in vitro is unknown. We found that the PA system components are expressed at very low levels in undifferentiated ESCs and that upon differentiation uPA activity is detected mainly transiently, whereas tPA activity and PAI-1 protein are maximum in well differentiated cells. Adipocyte formation by ESCs is inhibited by amiloride treatment, a specific uPA inhibitor. Likewise, ESCs expressing ectopic PAI-1 under the control of an inducible expression system display reduced adipogenic capacities after induction of the gene. Furthermore, the adipogenic differentiation capacities of PAI-1(-/-) induced pluripotent stem cells (iPSCs) are augmented as compared to wt iPSCs. Our results demonstrate that the control of ESC adipogenesis by the PA system correspond to different successive steps from undifferentiated to well differentiated ESCs. Similarly, skeletal myogenesis is decreased by uPA inhibition or PAI-1 overexpression during the terminal step of differentiation. However, interfering with uPA during days 0 to 3 of the differentiation process augments ESC myotube formation. Neither neurogenesis, cardiomyogenesis, endothelial cell nor smooth muscle formation are affected by amiloride or PAI-1 induction. Our results show that the PA system is capable to specifically modulate adipogenesis and skeletal myogenesis of ESCs by successive different molecular mechanisms.
format	article
author	Ola Hadadeh Emilie Barruet Franck Peiretti Monique Verdier Denis Bernot Yasmine Hadjal Claire El Yazidi Andrée Robaglia-Schlupp Andre Maues De Paula Didier Nègre Michelina Iacovino Michael Kyba Marie-Christine Alessi Bernard Binétruy
author_facet	Ola Hadadeh Emilie Barruet Franck Peiretti Monique Verdier Denis Bernot Yasmine Hadjal Claire El Yazidi Andrée Robaglia-Schlupp Andre Maues De Paula Didier Nègre Michelina Iacovino Michael Kyba Marie-Christine Alessi Bernard Binétruy
author_sort	Ola Hadadeh
title	The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.
title_short	The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.
title_full	The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.
title_fullStr	The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.
title_full_unstemmed	The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.
title_sort	plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1e5f7d84cb734b4dba74ed68d267a65e
work_keys_str_mv	AT olahadadeh theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT emiliebarruet theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT franckpeiretti theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT moniqueverdier theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT denisbernot theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT yasminehadjal theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT claireelyazidi theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT andreerobagliaschlupp theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT andremauesdepaula theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT didiernegre theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT michelinaiacovino theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT michaelkyba theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT mariechristinealessi theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT bernardbinetruy theplasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT olahadadeh plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT emiliebarruet plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT franckpeiretti plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT moniqueverdier plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT denisbernot plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT yasminehadjal plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT claireelyazidi plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT andreerobagliaschlupp plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT andremauesdepaula plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT didiernegre plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT michelinaiacovino plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT michaelkyba plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT mariechristinealessi plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells AT bernardbinetruy plasminogenactivationsystemmodulatesdifferentlyadipogenesisandmyogenesisofembryonicstemcells
_version_	1718422092697829376

The plasminogen activation system modulates differently adipogenesis and myogenesis of embryonic stem cells.

Ejemplares similares