Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems

Despite the role of γ-aminobutyric acid (GABA) in plant tolerance to chilling stress having been widely discussed in the seedling stage, very little information is clear regarding its implication in chilling tolerance during the reproductive stage of the plant. Here, we investigated the influence of...

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Autores principales:	Ola H. Abd Elbar, Amr Elkelish, Gniewko Niedbała, Reham Farag, Tomasz Wojciechowski, Soumya Mukherjee, Ayman F. Abou-Hadid, Hussien M. El-Hennawy, Ahmed Abou El-Yazied, Hany G. Abd El-Gawad, Ehab Azab, Adil A. Gobouri, Nihal El Nahhas, Ahmed M. El-Sawy, Ahmed Bondok, Mohamed F. M. Ibrahim
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	tomato (Solanum lycopersicum L.) gamma-aminobutyric acid chilling stress chloroplast ultrastructure oxidative stress antioxidants Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/1e635d787dd849ce9120b3d4630aed50
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spelling	oai:doaj.org-article:1e635d787dd849ce9120b3d4630aed502021-11-19T17:57:56ZProtective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems1664-462X10.3389/fpls.2021.663750https://doaj.org/article/1e635d787dd849ce9120b3d4630aed502021-10-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.663750/fullhttps://doaj.org/toc/1664-462XDespite the role of γ-aminobutyric acid (GABA) in plant tolerance to chilling stress having been widely discussed in the seedling stage, very little information is clear regarding its implication in chilling tolerance during the reproductive stage of the plant. Here, we investigated the influence of GABA (1 and 2mM) as a foliar application on tomato plants (Solanum lycopersicum L. cv. Super Marmande) subjected to chilling stress (5°C for 6h/day) for 5 successive days during the flowering stage. The results indicated that applied GABA differentially influenced leaf pigment composition by decreasing the chlorophyll a/b ratio and increasing the anthocyanin relative to total chlorophyll. However, carotenoids were not affected in both GABA-treated and non-treated stressed plants. Root tissues significantly exhibited an increase in thermo-tolerance in GABA-treated plants. Furthermore, applied GABA substantially alleviated the chilling-induced oxidative damage by protecting cell membrane integrity and reducing malondialdehyde (MDA) and H2O2. This positive effect of GABA was associated with enhancing the activity of phenylalanine ammonia-lyase (PAL), catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX). Conversely, a downregulation of peroxidase (POX) and polyphenol oxidase (PPO) was observed under chilling stress which indicates its relevance in phenol metabolism. Interesting correlations were obtained between GABA-induced upregulation of sugar metabolism coinciding with altering secondary metabolism, activities of antioxidant enzymes, and maintaining the integrity of plastids’ ultrastructure Eventually, applied GABA especially at 2mM improved the fruit yield and could be recommended to mitigate the damage of chilling stress in tomato plants.Ola H. Abd ElbarAmr ElkelishGniewko NiedbałaReham FaragTomasz WojciechowskiSoumya MukherjeeAyman F. Abou-HadidHussien M. El-HennawyAhmed Abou El-YaziedHany G. Abd El-GawadEhab AzabAdil A. GobouriNihal El NahhasAhmed M. El-SawyAhmed BondokMohamed F. M. IbrahimFrontiers Media S.A.articletomato (Solanum lycopersicum L.)gamma-aminobutyric acidchilling stresschloroplast ultrastructureoxidative stressantioxidantsPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	tomato (Solanum lycopersicum L.) gamma-aminobutyric acid chilling stress chloroplast ultrastructure oxidative stress antioxidants Plant culture SB1-1110
spellingShingle	tomato (Solanum lycopersicum L.) gamma-aminobutyric acid chilling stress chloroplast ultrastructure oxidative stress antioxidants Plant culture SB1-1110 Ola H. Abd Elbar Amr Elkelish Gniewko Niedbała Reham Farag Tomasz Wojciechowski Soumya Mukherjee Ayman F. Abou-Hadid Hussien M. El-Hennawy Ahmed Abou El-Yazied Hany G. Abd El-Gawad Ehab Azab Adil A. Gobouri Nihal El Nahhas Ahmed M. El-Sawy Ahmed Bondok Mohamed F. M. Ibrahim Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems
description	Despite the role of γ-aminobutyric acid (GABA) in plant tolerance to chilling stress having been widely discussed in the seedling stage, very little information is clear regarding its implication in chilling tolerance during the reproductive stage of the plant. Here, we investigated the influence of GABA (1 and 2mM) as a foliar application on tomato plants (Solanum lycopersicum L. cv. Super Marmande) subjected to chilling stress (5°C for 6h/day) for 5 successive days during the flowering stage. The results indicated that applied GABA differentially influenced leaf pigment composition by decreasing the chlorophyll a/b ratio and increasing the anthocyanin relative to total chlorophyll. However, carotenoids were not affected in both GABA-treated and non-treated stressed plants. Root tissues significantly exhibited an increase in thermo-tolerance in GABA-treated plants. Furthermore, applied GABA substantially alleviated the chilling-induced oxidative damage by protecting cell membrane integrity and reducing malondialdehyde (MDA) and H2O2. This positive effect of GABA was associated with enhancing the activity of phenylalanine ammonia-lyase (PAL), catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX). Conversely, a downregulation of peroxidase (POX) and polyphenol oxidase (PPO) was observed under chilling stress which indicates its relevance in phenol metabolism. Interesting correlations were obtained between GABA-induced upregulation of sugar metabolism coinciding with altering secondary metabolism, activities of antioxidant enzymes, and maintaining the integrity of plastids’ ultrastructure Eventually, applied GABA especially at 2mM improved the fruit yield and could be recommended to mitigate the damage of chilling stress in tomato plants.
format	article
author	Ola H. Abd Elbar Amr Elkelish Gniewko Niedbała Reham Farag Tomasz Wojciechowski Soumya Mukherjee Ayman F. Abou-Hadid Hussien M. El-Hennawy Ahmed Abou El-Yazied Hany G. Abd El-Gawad Ehab Azab Adil A. Gobouri Nihal El Nahhas Ahmed M. El-Sawy Ahmed Bondok Mohamed F. M. Ibrahim
author_facet	Ola H. Abd Elbar Amr Elkelish Gniewko Niedbała Reham Farag Tomasz Wojciechowski Soumya Mukherjee Ayman F. Abou-Hadid Hussien M. El-Hennawy Ahmed Abou El-Yazied Hany G. Abd El-Gawad Ehab Azab Adil A. Gobouri Nihal El Nahhas Ahmed M. El-Sawy Ahmed Bondok Mohamed F. M. Ibrahim
author_sort	Ola H. Abd Elbar
title	Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems
title_short	Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems
title_full	Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems
title_fullStr	Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems
title_full_unstemmed	Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems
title_sort	protective effect of γ-aminobutyric acid against chilling stress during reproductive stage in tomato plants through modulation of sugar metabolism, chloroplast integrity, and antioxidative defense systems
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1e635d787dd849ce9120b3d4630aed50
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Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems

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