Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation

Abstract We evaluated the role of NLR family pyrin domain containing 3 (NLRP3) inflammasome in sterile corneal inflammation caused by lipopolysaccharide (LPS) or alkali burns in C57BL6 mice or NLRP3 KO (Nlrp3 −/− ) mice. Various molecules related to the NLRP3 inflammasome were upregulated in C57BL6...

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Autores principales:	Hiroaki Shimizu, Tohru Sakimoto, Satoru Yamagami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e6d012662cf4412b5caad81d7c2418a
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spelling	oai:doaj.org-article:1e6d012662cf4412b5caad81d7c2418a2021-12-02T16:08:04ZPro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation10.1038/s41598-019-46116-92045-2322https://doaj.org/article/1e6d012662cf4412b5caad81d7c2418a2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46116-9https://doaj.org/toc/2045-2322Abstract We evaluated the role of NLR family pyrin domain containing 3 (NLRP3) inflammasome in sterile corneal inflammation caused by lipopolysaccharide (LPS) or alkali burns in C57BL6 mice or NLRP3 KO (Nlrp3 −/− ) mice. Various molecules related to the NLRP3 inflammasome were upregulated in C57BL6 mice after both alkali burn injury and LPS treatment. After alkali burn injury, the corneal opacity grade was significantly reduced in Nlrp3 −/− mice compared with C57BL6 mice. In Nlrp3 −/− mice, Gr-1 immunoreactivity and MMP-9 mRNA expression in the corneal stroma were significantly reduced by both LPS treatment and alkali burn injury. Quantitative PCR and immunohistochemistry revealed that IL-1β and MMP-9 expression in the corneal stroma were down-regulated in Nlrp3 −/− mice with both alkali burn injury and LPS treatment. These findings suggest that the NLRP3 inflammasome has a pro-inflammatory effect in the cornea by recruiting neutrophils to sites of inflammation.Hiroaki ShimizuTohru SakimotoSatoru YamagamiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroaki Shimizu Tohru Sakimoto Satoru Yamagami Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation
description	Abstract We evaluated the role of NLR family pyrin domain containing 3 (NLRP3) inflammasome in sterile corneal inflammation caused by lipopolysaccharide (LPS) or alkali burns in C57BL6 mice or NLRP3 KO (Nlrp3 −/− ) mice. Various molecules related to the NLRP3 inflammasome were upregulated in C57BL6 mice after both alkali burn injury and LPS treatment. After alkali burn injury, the corneal opacity grade was significantly reduced in Nlrp3 −/− mice compared with C57BL6 mice. In Nlrp3 −/− mice, Gr-1 immunoreactivity and MMP-9 mRNA expression in the corneal stroma were significantly reduced by both LPS treatment and alkali burn injury. Quantitative PCR and immunohistochemistry revealed that IL-1β and MMP-9 expression in the corneal stroma were down-regulated in Nlrp3 −/− mice with both alkali burn injury and LPS treatment. These findings suggest that the NLRP3 inflammasome has a pro-inflammatory effect in the cornea by recruiting neutrophils to sites of inflammation.
format	article
author	Hiroaki Shimizu Tohru Sakimoto Satoru Yamagami
author_facet	Hiroaki Shimizu Tohru Sakimoto Satoru Yamagami
author_sort	Hiroaki Shimizu
title	Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation
title_short	Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation
title_full	Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation
title_fullStr	Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation
title_full_unstemmed	Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation
title_sort	pro-inflammatory role of nlrp3 inflammasome in experimental sterile corneal inflammation
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/1e6d012662cf4412b5caad81d7c2418a
work_keys_str_mv	AT hiroakishimizu proinflammatoryroleofnlrp3inflammasomeinexperimentalsterilecornealinflammation AT tohrusakimoto proinflammatoryroleofnlrp3inflammasomeinexperimentalsterilecornealinflammation AT satoruyamagami proinflammatoryroleofnlrp3inflammasomeinexperimentalsterilecornealinflammation
_version_	1718384641494220800

Pro-inflammatory role of NLRP3 inflammasome in experimental sterile corneal inflammation

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