Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine

Abstract The initiator caspase Dronc is the only CARD-domain containing caspase in Drosophila and is essential for apoptosis. Here, we report that homozygous dronc mutant adult animals are short-lived due to the presence of a poorly developed, defective and leaky intestine. Interestingly, this mutan...

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Autores principales:	Jillian L. Lindblad, Meghana Tare, Alla Amcheslavsky, Alicia Shields, Andreas Bergmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e7b8635b7c346bc9c000cad7a5eb057
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spelling	oai:doaj.org-article:1e7b8635b7c346bc9c000cad7a5eb0572021-12-02T14:16:57ZNon-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine10.1038/s41598-021-81261-02045-2322https://doaj.org/article/1e7b8635b7c346bc9c000cad7a5eb0572021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81261-0https://doaj.org/toc/2045-2322Abstract The initiator caspase Dronc is the only CARD-domain containing caspase in Drosophila and is essential for apoptosis. Here, we report that homozygous dronc mutant adult animals are short-lived due to the presence of a poorly developed, defective and leaky intestine. Interestingly, this mutant phenotype can be significantly rescued by enteroblast-specific expression of dronc + in dronc mutant animals, suggesting that proper Dronc function specifically in enteroblasts, one of four cell types in the intestine, is critical for normal development of the intestine. Furthermore, enteroblast-specific knockdown of dronc in adult intestines triggers hyperplasia and differentiation defects. These enteroblast-specific functions of Dronc do not require the apoptotic pathway and thus occur in a non-apoptotic manner. In summary, we demonstrate that an apoptotic initiator caspase has a very critical non-apoptotic function for normal development and for the control of the cell lineage in the adult midgut and therefore for proper physiology and homeostasis.Jillian L. LindbladMeghana TareAlla AmcheslavskyAlicia ShieldsAndreas BergmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jillian L. Lindblad Meghana Tare Alla Amcheslavsky Alicia Shields Andreas Bergmann Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine
description	Abstract The initiator caspase Dronc is the only CARD-domain containing caspase in Drosophila and is essential for apoptosis. Here, we report that homozygous dronc mutant adult animals are short-lived due to the presence of a poorly developed, defective and leaky intestine. Interestingly, this mutant phenotype can be significantly rescued by enteroblast-specific expression of dronc + in dronc mutant animals, suggesting that proper Dronc function specifically in enteroblasts, one of four cell types in the intestine, is critical for normal development of the intestine. Furthermore, enteroblast-specific knockdown of dronc in adult intestines triggers hyperplasia and differentiation defects. These enteroblast-specific functions of Dronc do not require the apoptotic pathway and thus occur in a non-apoptotic manner. In summary, we demonstrate that an apoptotic initiator caspase has a very critical non-apoptotic function for normal development and for the control of the cell lineage in the adult midgut and therefore for proper physiology and homeostasis.
format	article
author	Jillian L. Lindblad Meghana Tare Alla Amcheslavsky Alicia Shields Andreas Bergmann
author_facet	Jillian L. Lindblad Meghana Tare Alla Amcheslavsky Alicia Shields Andreas Bergmann
author_sort	Jillian L. Lindblad
title	Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine
title_short	Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine
title_full	Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine
title_fullStr	Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine
title_full_unstemmed	Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine
title_sort	non-apoptotic enteroblast-specific role of the initiator caspase dronc for development and homeostasis of the drosophila intestine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1e7b8635b7c346bc9c000cad7a5eb057
work_keys_str_mv	AT jillianllindblad nonapoptoticenteroblastspecificroleoftheinitiatorcaspasedroncfordevelopmentandhomeostasisofthedrosophilaintestine AT meghanatare nonapoptoticenteroblastspecificroleoftheinitiatorcaspasedroncfordevelopmentandhomeostasisofthedrosophilaintestine AT allaamcheslavsky nonapoptoticenteroblastspecificroleoftheinitiatorcaspasedroncfordevelopmentandhomeostasisofthedrosophilaintestine AT aliciashields nonapoptoticenteroblastspecificroleoftheinitiatorcaspasedroncfordevelopmentandhomeostasisofthedrosophilaintestine AT andreasbergmann nonapoptoticenteroblastspecificroleoftheinitiatorcaspasedroncfordevelopmentandhomeostasisofthedrosophilaintestine
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Non-apoptotic enteroblast-specific role of the initiator caspase Dronc for development and homeostasis of the Drosophila intestine

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