Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells

Jong Hwa Jun,1 Wern-Joo Sohn,2 Youngkyun Lee,2 Jae-Young Kim21Department of Ophthalmology, School of Medicine, Dongsan Medical Center, Keimyung University, 2Department of Oral Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South KoreaAbstract: The molecular a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun JH, Sohn WJ, Lee Y, Kim JY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Avastin bevacizumab lens epithelial cell transforming growth factor vascular endothelial growth factor Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/1e7ef79f18d54570841eaea4dcb062fb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1e7ef79f18d54570841eaea4dcb062fb
record_format	dspace
spelling	oai:doaj.org-article:1e7ef79f18d54570841eaea4dcb062fb2021-12-02T01:57:46ZEffects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells1177-5483https://doaj.org/article/1e7ef79f18d54570841eaea4dcb062fb2016-06-01T00:00:00Zhttps://www.dovepress.com/effects-of-anti-vascular-endothelial-growth-factor-monoclonal-antibody-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Jong Hwa Jun,1 Wern-Joo Sohn,2 Youngkyun Lee,2 Jae-Young Kim21Department of Ophthalmology, School of Medicine, Dongsan Medical Center, Keimyung University, 2Department of Oral Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South KoreaAbstract: The molecular and cellular effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells (LECs) were examined using both an immortalized human lens epithelial cell line and a porcine capsular bag model. After treatment with various concentrations of bevacizumab, cell viability and proliferation patterns were evaluated using the water-soluble tetrazolium salt assay and 5-bromo-2'-deoxyuridine enzyme-linked immunosorbent assay, respectively. The scratch assay and Western blot analysis were employed to validate the cell migration pattern and altered expression levels of signaling molecules related to the epithelial–mesenchymal transition (EMT). Application of bevacizumab induced a range of altered cellular events in a concentration-dependent manner. A 0.1–2 mg/mL concentration demonstrated dose-dependent increase in proliferation and viability of LECs. However, 4 mg/mL decreased cell proliferation and viability. Cell migrations displayed dose-dependent retardation from 0.1 mg/mL bevacizumab treatment. Transforming growth factor-β2 expression was markedly increased in a dose-dependent manner, and α-smooth muscle actin, matrix metalloproteinase-9, and vimentin expression levels showed dose-dependent changes in a B3 cell line. Microscopic observation of porcine capsular bag revealed changes in cellular morphology and a decline in cell density compared to the control after 2 mg/mL treatment. The central aspect of posterior capsule showed delayed confluence, and the factors related to EMT revealed similar expression patterns to those identified in the cell line. Based on these results, bevacizumab modulates the proliferation and viability of LECs and induces morphological alterations through the modulation of expression patterns of specific factors related to the EMT.Keywords: avastin, bevacizumab, lens epithelial cell, transforming growth factor, vascular endothelial growth factorJun JHSohn WJLee YKim JYDove Medical PressarticleAvastinbevacizumablens epithelial celltransforming growth factorvascular endothelial growth factorOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2016, Iss Issue 1, Pp 1167-1174 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Avastin bevacizumab lens epithelial cell transforming growth factor vascular endothelial growth factor Ophthalmology RE1-994
spellingShingle	Avastin bevacizumab lens epithelial cell transforming growth factor vascular endothelial growth factor Ophthalmology RE1-994 Jun JH Sohn WJ Lee Y Kim JY Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
description	Jong Hwa Jun,1 Wern-Joo Sohn,2 Youngkyun Lee,2 Jae-Young Kim21Department of Ophthalmology, School of Medicine, Dongsan Medical Center, Keimyung University, 2Department of Oral Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South KoreaAbstract: The molecular and cellular effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells (LECs) were examined using both an immortalized human lens epithelial cell line and a porcine capsular bag model. After treatment with various concentrations of bevacizumab, cell viability and proliferation patterns were evaluated using the water-soluble tetrazolium salt assay and 5-bromo-2'-deoxyuridine enzyme-linked immunosorbent assay, respectively. The scratch assay and Western blot analysis were employed to validate the cell migration pattern and altered expression levels of signaling molecules related to the epithelial–mesenchymal transition (EMT). Application of bevacizumab induced a range of altered cellular events in a concentration-dependent manner. A 0.1–2 mg/mL concentration demonstrated dose-dependent increase in proliferation and viability of LECs. However, 4 mg/mL decreased cell proliferation and viability. Cell migrations displayed dose-dependent retardation from 0.1 mg/mL bevacizumab treatment. Transforming growth factor-β2 expression was markedly increased in a dose-dependent manner, and α-smooth muscle actin, matrix metalloproteinase-9, and vimentin expression levels showed dose-dependent changes in a B3 cell line. Microscopic observation of porcine capsular bag revealed changes in cellular morphology and a decline in cell density compared to the control after 2 mg/mL treatment. The central aspect of posterior capsule showed delayed confluence, and the factors related to EMT revealed similar expression patterns to those identified in the cell line. Based on these results, bevacizumab modulates the proliferation and viability of LECs and induces morphological alterations through the modulation of expression patterns of specific factors related to the EMT.Keywords: avastin, bevacizumab, lens epithelial cell, transforming growth factor, vascular endothelial growth factor
format	article
author	Jun JH Sohn WJ Lee Y Kim JY
author_facet	Jun JH Sohn WJ Lee Y Kim JY
author_sort	Jun JH
title	Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
title_short	Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
title_full	Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
title_fullStr	Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
title_full_unstemmed	Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
title_sort	effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/1e7ef79f18d54570841eaea4dcb062fb
work_keys_str_mv	AT junjh effectsofantivascularendothelialgrowthfactormonoclonalantibodybevacizumabonlensepithelialcells AT sohnwj effectsofantivascularendothelialgrowthfactormonoclonalantibodybevacizumabonlensepithelialcells AT leey effectsofantivascularendothelialgrowthfactormonoclonalantibodybevacizumabonlensepithelialcells AT kimjy effectsofantivascularendothelialgrowthfactormonoclonalantibodybevacizumabonlensepithelialcells
_version_	1718402761091973120

Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells

Ejemplares similares