CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part...

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Autores principales:	Nasir Javaid, Sangdun Choi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	CRISPR/cas system classification host DNA repair genome-editing efficiency epigenome Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1e816245f1ed4f54ad63bf708076edde
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id	oai:doaj.org-article:1e816245f1ed4f54ad63bf708076edde
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spelling	oai:doaj.org-article:1e816245f1ed4f54ad63bf708076edde2021-11-30T19:00:18ZCRISPR/Cas System and Factors Affecting Its Precision and Efficiency2296-634X10.3389/fcell.2021.761709https://doaj.org/article/1e816245f1ed4f54ad63bf708076edde2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.761709/fullhttps://doaj.org/toc/2296-634XThe diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.Nasir JavaidSangdun ChoiSangdun ChoiFrontiers Media S.A.articleCRISPR/cas systemclassificationhost DNA repairgenome-editing efficiencyepigenomeBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CRISPR/cas system classification host DNA repair genome-editing efficiency epigenome Biology (General) QH301-705.5
spellingShingle	CRISPR/cas system classification host DNA repair genome-editing efficiency epigenome Biology (General) QH301-705.5 Nasir Javaid Sangdun Choi Sangdun Choi CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
description	The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.
format	article
author	Nasir Javaid Sangdun Choi Sangdun Choi
author_facet	Nasir Javaid Sangdun Choi Sangdun Choi
author_sort	Nasir Javaid
title	CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_short	CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_full	CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_fullStr	CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_full_unstemmed	CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
title_sort	crispr/cas system and factors affecting its precision and efficiency
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/1e816245f1ed4f54ad63bf708076edde
work_keys_str_mv	AT nasirjavaid crisprcassystemandfactorsaffectingitsprecisionandefficiency AT sangdunchoi crisprcassystemandfactorsaffectingitsprecisionandefficiency AT sangdunchoi crisprcassystemandfactorsaffectingitsprecisionandefficiency
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CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

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