Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.

With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was...

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Autores principales:	Alireza Saraji, Anne Offermann, Janine Stegmann-Frehse, Katharina Hempel, Duan Kang, Rosemarie Krupar, Christian Watermann, Danny Jonigk, Mark Philipp Kühnel, Jutta Kirfel, Sven Perner, Verena Sailer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e828c583f6242608f0c9b44085808a4
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spelling	oai:doaj.org-article:1e828c583f6242608f0c9b44085808a42021-12-02T20:14:34ZCracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.1932-620310.1371/journal.pone.0257416https://doaj.org/article/1e828c583f6242608f0c9b44085808a42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257416https://doaj.org/toc/1932-6203With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.Alireza SarajiAnne OffermannJanine Stegmann-FrehseKatharina HempelDuan KangRosemarie KruparChristian WatermannDanny JonigkMark Philipp KühnelJutta KirfelSven PernerVerena SailerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257416 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alireza Saraji Anne Offermann Janine Stegmann-Frehse Katharina Hempel Duan Kang Rosemarie Krupar Christian Watermann Danny Jonigk Mark Philipp Kühnel Jutta Kirfel Sven Perner Verena Sailer Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
description	With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.
format	article
author	Alireza Saraji Anne Offermann Janine Stegmann-Frehse Katharina Hempel Duan Kang Rosemarie Krupar Christian Watermann Danny Jonigk Mark Philipp Kühnel Jutta Kirfel Sven Perner Verena Sailer
author_facet	Alireza Saraji Anne Offermann Janine Stegmann-Frehse Katharina Hempel Duan Kang Rosemarie Krupar Christian Watermann Danny Jonigk Mark Philipp Kühnel Jutta Kirfel Sven Perner Verena Sailer
author_sort	Alireza Saraji
title	Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
title_short	Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
title_full	Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
title_fullStr	Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
title_full_unstemmed	Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
title_sort	cracking it - successful mrna extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1e828c583f6242608f0c9b44085808a4
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Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.

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