A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au

Abstract Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-...

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Autores principales:	Mohadeseh Zarei-Ghobadi, Sayed-Hamidreza Mozhgani, Fariba Dashtestani, Amir Yadegari, Fatemeh Hakimian, Mehdi Norouzi, Hedayatollah Ghourchian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Carbon Dots (CDs) CDs Fluorescence Biosensors FRET Efficiency Förster Resonance Energy Transfer (FRET) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e8f3db8911e4b82971b69174923d6bb
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spelling	oai:doaj.org-article:1e8f3db8911e4b82971b69174923d6bb2021-12-02T15:08:23ZA genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au10.1038/s41598-018-32756-w2045-2322https://doaj.org/article/1e8f3db8911e4b82971b69174923d6bb2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32756-whttps://doaj.org/toc/2045-2322Abstract Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-lymphotropic virus type 1 genome. One of the probes was covalently bound to the CDs. In the absence of target, CDs-probe was adsorbed on the surface of Fe3O4@Au through two possible mechanisms, leading to quenching the fluorescence emission of CDs. The fluorescence emission of CDs was recovered in the presence of target since double-stranded DNA cannot adsorb on the Fe3O4@Au. Also, Fe3O4@Au can adsorb the unhybridized oligonucleotides and improves the accuracy of detection. The specificity of the proposed biosensor was confirmed by BLAST search and assessed by exposing the biosensor to other virus targets. The experimental detection limit of the biosensor was below 10 nM with linear range from 10 to 320 nM.Mohadeseh Zarei-GhobadiSayed-Hamidreza MozhganiFariba DashtestaniAmir YadegariFatemeh HakimianMehdi NorouziHedayatollah GhourchianNature PortfolioarticleCarbon Dots (CDs)CDs FluorescenceBiosensorsFRET EfficiencyFörster Resonance Energy Transfer (FRET)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carbon Dots (CDs) CDs Fluorescence Biosensors FRET Efficiency Förster Resonance Energy Transfer (FRET) Medicine R Science Q
spellingShingle	Carbon Dots (CDs) CDs Fluorescence Biosensors FRET Efficiency Förster Resonance Energy Transfer (FRET) Medicine R Science Q Mohadeseh Zarei-Ghobadi Sayed-Hamidreza Mozhgani Fariba Dashtestani Amir Yadegari Fatemeh Hakimian Mehdi Norouzi Hedayatollah Ghourchian A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au
description	Abstract Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-lymphotropic virus type 1 genome. One of the probes was covalently bound to the CDs. In the absence of target, CDs-probe was adsorbed on the surface of Fe3O4@Au through two possible mechanisms, leading to quenching the fluorescence emission of CDs. The fluorescence emission of CDs was recovered in the presence of target since double-stranded DNA cannot adsorb on the Fe3O4@Au. Also, Fe3O4@Au can adsorb the unhybridized oligonucleotides and improves the accuracy of detection. The specificity of the proposed biosensor was confirmed by BLAST search and assessed by exposing the biosensor to other virus targets. The experimental detection limit of the biosensor was below 10 nM with linear range from 10 to 320 nM.
format	article
author	Mohadeseh Zarei-Ghobadi Sayed-Hamidreza Mozhgani Fariba Dashtestani Amir Yadegari Fatemeh Hakimian Mehdi Norouzi Hedayatollah Ghourchian
author_facet	Mohadeseh Zarei-Ghobadi Sayed-Hamidreza Mozhgani Fariba Dashtestani Amir Yadegari Fatemeh Hakimian Mehdi Norouzi Hedayatollah Ghourchian
author_sort	Mohadeseh Zarei-Ghobadi
title	A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au
title_short	A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au
title_full	A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au
title_fullStr	A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au
title_full_unstemmed	A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au
title_sort	genosensor for detection of htlv-i based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped au
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1e8f3db8911e4b82971b69174923d6bb
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A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au

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