Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences

Abstract Upon exposure to sunlight extracellular polymeric substances (EPS) were partially transformed into transparent exopolymer particles (TEP) and unstable flocs of different sizes without the addition of any precursors. Parallel factor (PARAFAC) modelling of the sample fluorescence spectra iden...

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Autores principales:	Mashura Shammi, Xiangliang Pan, Khan M. G. Mostofa, Daoyong Zhang, Cong-Qiang Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e94c167627b44e89e85768055cfaa0f
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spelling	oai:doaj.org-article:1e94c167627b44e89e85768055cfaa0f2021-12-02T11:40:42ZPhoto-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences10.1038/s41598-017-09066-82045-2322https://doaj.org/article/1e94c167627b44e89e85768055cfaa0f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09066-8https://doaj.org/toc/2045-2322Abstract Upon exposure to sunlight extracellular polymeric substances (EPS) were partially transformed into transparent exopolymer particles (TEP) and unstable flocs of different sizes without the addition of any precursors. Parallel factor (PARAFAC) modelling of the sample fluorescence spectra identified humic-like and protein-like or tyrosine-like components in both untreated and irradiated EPS samples. After 58 hours of solar irradiation, humic-like substances were entirely decomposed, while the regenerated protein-like substance from EPS was the key component in the irradiated samples. Degradation and reformation of EPS occurred which was confirmed by the results of size exclusion chromatography, dissolved organic carbon, total protein and total polysaccharide analyses. Irradiated EPS was composed of –COOH or C = O (amide I band) and –NH and –CN (amide II band), while Fourier transform infrared spectroscopy (FTIR) of TEP revealed more acidic –COOH and –C–O groups, indicating typical acidic protein-like TEP. The regenerated protein-like substances could form complexes with free metals originating from degraded EPS in irradiated samples, which could be responsible for the formation of TEP/floc in the aqueous media. These results suggest that TEP/floc formation from EPS could occur by a complexation mechanism between dissolved organic matter and metals, thereby causing ionic charge neutralisation upon sunlight exposure.Mashura ShammiXiangliang PanKhan M. G. MostofaDaoyong ZhangCong-Qiang LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mashura Shammi Xiangliang Pan Khan M. G. Mostofa Daoyong Zhang Cong-Qiang Liu Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences
description	Abstract Upon exposure to sunlight extracellular polymeric substances (EPS) were partially transformed into transparent exopolymer particles (TEP) and unstable flocs of different sizes without the addition of any precursors. Parallel factor (PARAFAC) modelling of the sample fluorescence spectra identified humic-like and protein-like or tyrosine-like components in both untreated and irradiated EPS samples. After 58 hours of solar irradiation, humic-like substances were entirely decomposed, while the regenerated protein-like substance from EPS was the key component in the irradiated samples. Degradation and reformation of EPS occurred which was confirmed by the results of size exclusion chromatography, dissolved organic carbon, total protein and total polysaccharide analyses. Irradiated EPS was composed of –COOH or C = O (amide I band) and –NH and –CN (amide II band), while Fourier transform infrared spectroscopy (FTIR) of TEP revealed more acidic –COOH and –C–O groups, indicating typical acidic protein-like TEP. The regenerated protein-like substances could form complexes with free metals originating from degraded EPS in irradiated samples, which could be responsible for the formation of TEP/floc in the aqueous media. These results suggest that TEP/floc formation from EPS could occur by a complexation mechanism between dissolved organic matter and metals, thereby causing ionic charge neutralisation upon sunlight exposure.
format	article
author	Mashura Shammi Xiangliang Pan Khan M. G. Mostofa Daoyong Zhang Cong-Qiang Liu
author_facet	Mashura Shammi Xiangliang Pan Khan M. G. Mostofa Daoyong Zhang Cong-Qiang Liu
author_sort	Mashura Shammi
title	Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences
title_short	Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences
title_full	Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences
title_fullStr	Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences
title_full_unstemmed	Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences
title_sort	photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: chemical and spectroscopic evidences
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1e94c167627b44e89e85768055cfaa0f
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Photo-flocculation of microbial mat extracellular polymeric substances and their transformation into transparent exopolymer particles: Chemical and spectroscopic evidences

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