The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction

Abstract Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising altern...

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Autores principales:	Chenxi Li, Xiaoqing Hu, Qingyang Meng, Xin Zhang, Jingxian Zhu, Linghui Dai, Jin Cheng, Mingjin Zhong, Weili Shi, Bo Ren, Jiying Zhang, Xin Fu, Xiaoning Duan, Yingfang Ao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e9553bdb3ba413cbc932092f43d2c53
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spelling	oai:doaj.org-article:1e9553bdb3ba413cbc932092f43d2c532021-12-02T11:53:00ZThe potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction10.1038/s41598-017-07166-z2045-2322https://doaj.org/article/1e9553bdb3ba413cbc932092f43d2c532017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07166-zhttps://doaj.org/toc/2045-2322Abstract Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising alternative for tissue engineering. Among them, autograft is a preferred option for its safety and excellent biocompatibility. In this study, we utilized semitendinosus tendon autograft in meniscus reconstruction to investigate its fibrochondrogenic metaplasticity potential and chondroprotective effect. Tendon-derived stem cells (TDSCs) and synovial-derived mesenchymal stem cells (SMSCs), two most important stem cell sources in our strategy, exhibited excellent viability, distribution, proliferation and fibrochondrogenic differentiation ability in decellularized semitendinosus tendon (DST) scaffolds in vitro. Histologic evaluation of the tendon grafts in vivo suggested endogenous stem cells differentiated into fibrochondrocytes, synthesized proteoglycan, type II collagen and radial type I collagen at 12 weeks and 24 weeks post-surgery. As for elastic modulus and hardness of the grafts, there were no significant differences between native meniscus and regenerated meniscus at 24 weeks. The protection of condylar cartilage from degeneration was significantly better in the reconstruction group comparing to control group. Overall, semitendinosus tendon autograft seems to be a promising substitution in meniscus reconstruction.Chenxi LiXiaoqing HuQingyang MengXin ZhangJingxian ZhuLinghui DaiJin ChengMingjin ZhongWeili ShiBo RenJiying ZhangXin FuXiaoning DuanYingfang AoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chenxi Li Xiaoqing Hu Qingyang Meng Xin Zhang Jingxian Zhu Linghui Dai Jin Cheng Mingjin Zhong Weili Shi Bo Ren Jiying Zhang Xin Fu Xiaoning Duan Yingfang Ao The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
description	Abstract Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising alternative for tissue engineering. Among them, autograft is a preferred option for its safety and excellent biocompatibility. In this study, we utilized semitendinosus tendon autograft in meniscus reconstruction to investigate its fibrochondrogenic metaplasticity potential and chondroprotective effect. Tendon-derived stem cells (TDSCs) and synovial-derived mesenchymal stem cells (SMSCs), two most important stem cell sources in our strategy, exhibited excellent viability, distribution, proliferation and fibrochondrogenic differentiation ability in decellularized semitendinosus tendon (DST) scaffolds in vitro. Histologic evaluation of the tendon grafts in vivo suggested endogenous stem cells differentiated into fibrochondrocytes, synthesized proteoglycan, type II collagen and radial type I collagen at 12 weeks and 24 weeks post-surgery. As for elastic modulus and hardness of the grafts, there were no significant differences between native meniscus and regenerated meniscus at 24 weeks. The protection of condylar cartilage from degeneration was significantly better in the reconstruction group comparing to control group. Overall, semitendinosus tendon autograft seems to be a promising substitution in meniscus reconstruction.
format	article
author	Chenxi Li Xiaoqing Hu Qingyang Meng Xin Zhang Jingxian Zhu Linghui Dai Jin Cheng Mingjin Zhong Weili Shi Bo Ren Jiying Zhang Xin Fu Xiaoning Duan Yingfang Ao
author_facet	Chenxi Li Xiaoqing Hu Qingyang Meng Xin Zhang Jingxian Zhu Linghui Dai Jin Cheng Mingjin Zhong Weili Shi Bo Ren Jiying Zhang Xin Fu Xiaoning Duan Yingfang Ao
author_sort	Chenxi Li
title	The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_short	The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_full	The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_fullStr	The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_full_unstemmed	The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
title_sort	potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1e9553bdb3ba413cbc932092f43d2c53
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The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction

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