Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.

<h4>Introduction</h4>Periprosthetic joint infection (PJI) represents a devastating complication of total joint arthroplasty associated with significant morbidity and mortality. Literature suggests a possible higher incidence of periprosthetic joint infection (PJI) in patients with rheuma...

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Autores principales:	Rishi Trikha, Danielle Greig, Troy Sekimura, Nicolas Cevallos, Benjamin Kelley, Zeinab Mamouei, Christopher Hart, Micah Ralston, Amr Turkmani, Adam Sassoon, Alexandra Stavrakis, Nicholas M Bernthal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e96fef351fb4a899dab5a7a56b4f720
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spelling	oai:doaj.org-article:1e96fef351fb4a899dab5a7a56b4f7202021-12-02T20:18:05ZActive rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.1932-620310.1371/journal.pone.0250910https://doaj.org/article/1e96fef351fb4a899dab5a7a56b4f7202021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0250910https://doaj.org/toc/1932-6203<h4>Introduction</h4>Periprosthetic joint infection (PJI) represents a devastating complication of total joint arthroplasty associated with significant morbidity and mortality. Literature suggests a possible higher incidence of periprosthetic joint infection (PJI) in patients with rheumatoid arthritis (RA). There is, however, no consensus on this purported risk nor a well-defined mechanism. This study investigates how collagen-induced arthritis (CIA), a validated animal model of RA, impacts infectious burden in a well-established model of PJI.<h4>Methods</h4>Control mice were compared against CIA mice. Whole blood samples were collected to quantify systemic IgG levels via ELISA. Ex vivo respiratory burst function was measured via dihydrorhodamine assay. Ex vivo Staphylococcus aureus Xen36 burden was measured directly via colony forming unit (CFU) counts and crystal violet assay to assess biofilm formation. In vivo, surgical placement of a titanium implant through the knee joint and inoculation with S. aureus Xen36 was performed. Bacterial burden was then quantified by longitudinal bioluminescent imaging.<h4>Results</h4>Mice with CIA demonstrated significantly higher levels of systemic IgG compared with control mice (p = 0.003). Ex vivo, there was no significant difference in respiratory burst function (p = 0.89) or S. aureus bacterial burden as measured by CFU counts (p = 0.91) and crystal violet assay (p = 0.96). In vivo, no significant difference in bacterial bioluminescence between groups was found at all postoperative time points. CFU counts of both the implant and the peri-implant tissue were not significantly different between groups (p = 0.82 and 0.80, respectively).<h4>Conclusion</h4>This study demonstrated no significant difference in S. aureus infectious burden between mice with CIA and control mice. These results suggest that untreated, active RA may not represent a significant intrinsic risk factor for PJI, however further mechanistic translational and clinical studies are warranted.Rishi TrikhaDanielle GreigTroy SekimuraNicolas CevallosBenjamin KelleyZeinab MamoueiChristopher HartMicah RalstonAmr TurkmaniAdam SassoonAlexandra StavrakisNicholas M BernthalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0250910 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rishi Trikha Danielle Greig Troy Sekimura Nicolas Cevallos Benjamin Kelley Zeinab Mamouei Christopher Hart Micah Ralston Amr Turkmani Adam Sassoon Alexandra Stavrakis Nicholas M Bernthal Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.
description	<h4>Introduction</h4>Periprosthetic joint infection (PJI) represents a devastating complication of total joint arthroplasty associated with significant morbidity and mortality. Literature suggests a possible higher incidence of periprosthetic joint infection (PJI) in patients with rheumatoid arthritis (RA). There is, however, no consensus on this purported risk nor a well-defined mechanism. This study investigates how collagen-induced arthritis (CIA), a validated animal model of RA, impacts infectious burden in a well-established model of PJI.<h4>Methods</h4>Control mice were compared against CIA mice. Whole blood samples were collected to quantify systemic IgG levels via ELISA. Ex vivo respiratory burst function was measured via dihydrorhodamine assay. Ex vivo Staphylococcus aureus Xen36 burden was measured directly via colony forming unit (CFU) counts and crystal violet assay to assess biofilm formation. In vivo, surgical placement of a titanium implant through the knee joint and inoculation with S. aureus Xen36 was performed. Bacterial burden was then quantified by longitudinal bioluminescent imaging.<h4>Results</h4>Mice with CIA demonstrated significantly higher levels of systemic IgG compared with control mice (p = 0.003). Ex vivo, there was no significant difference in respiratory burst function (p = 0.89) or S. aureus bacterial burden as measured by CFU counts (p = 0.91) and crystal violet assay (p = 0.96). In vivo, no significant difference in bacterial bioluminescence between groups was found at all postoperative time points. CFU counts of both the implant and the peri-implant tissue were not significantly different between groups (p = 0.82 and 0.80, respectively).<h4>Conclusion</h4>This study demonstrated no significant difference in S. aureus infectious burden between mice with CIA and control mice. These results suggest that untreated, active RA may not represent a significant intrinsic risk factor for PJI, however further mechanistic translational and clinical studies are warranted.
format	article
author	Rishi Trikha Danielle Greig Troy Sekimura Nicolas Cevallos Benjamin Kelley Zeinab Mamouei Christopher Hart Micah Ralston Amr Turkmani Adam Sassoon Alexandra Stavrakis Nicholas M Bernthal
author_facet	Rishi Trikha Danielle Greig Troy Sekimura Nicolas Cevallos Benjamin Kelley Zeinab Mamouei Christopher Hart Micah Ralston Amr Turkmani Adam Sassoon Alexandra Stavrakis Nicholas M Bernthal
author_sort	Rishi Trikha
title	Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.
title_short	Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.
title_full	Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.
title_fullStr	Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.
title_full_unstemmed	Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.
title_sort	active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1e96fef351fb4a899dab5a7a56b4f720
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Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.

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