Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.

Our aim was to isolate, identify and characterize probiotic bacteria as vitamin producers in particular B2 and B9. 150 human fecal samples were collected and used for isolation of vitamin producers-probiotics. 49 isolates were chosen for screening their genome by PCR for the presence of riboflavin a...

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Autores principales:	Nahla M Mansour, Wagiha S Elkalla, Yasser M Ragab, Mohamed A Ramadan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1e9b1308c06643c59cd12c4dafea6041
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spelling	oai:doaj.org-article:1e9b1308c06643c59cd12c4dafea60412021-12-02T20:06:27ZInhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.1932-620310.1371/journal.pone.0255092https://doaj.org/article/1e9b1308c06643c59cd12c4dafea60412021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255092https://doaj.org/toc/1932-6203Our aim was to isolate, identify and characterize probiotic bacteria as vitamin producers in particular B2 and B9. 150 human fecal samples were collected and used for isolation of vitamin producers-probiotics. 49 isolates were chosen for screening their genome by PCR for the presence of riboflavin and folic acid genes. As a result, three isolates were selected and their production of the B2 and B9 were confirmed by HPLC. The three isolates were identified on species level by sequencing their 16S rRNA gene which showed 100% identical to strains of Pediococcus acidilactici. Thus, they were named as P. acidilactici WNYM01, P. acidilactici WNYM02, P. acidilactici WNYM03 and submitted to the Genbank database with accession numbers. They met the probiotic criteria by expressing 90-95% survival rate at pH (2.0-9.0) and bile salt up to 2% for 3 h in addition to their antimicrobial activity against gram positive and negative microorganisms. They also showed no hemolytic activity and common pattern for antibiotic susceptibility. Our three strains were tested individually or in mixture in vivo on rat colitis model compared to ulcerative group. The strains were administrated orally to rats in daily dose containing CFU 109 for 14 days then followed by induction of colitis using acetic acid then the oral administration was continued for more four days. The histology results, the anti-inflammatory and anti-oxidative stress biomarkers showed the protective role of the strains compared to the ulcerative group. As a conclusion, we introduce novel three probiotic candidates for pharmaceutical preparations and health applications.Nahla M MansourWagiha S ElkallaYasser M RagabMohamed A RamadanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255092 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nahla M Mansour Wagiha S Elkalla Yasser M Ragab Mohamed A Ramadan Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
description	Our aim was to isolate, identify and characterize probiotic bacteria as vitamin producers in particular B2 and B9. 150 human fecal samples were collected and used for isolation of vitamin producers-probiotics. 49 isolates were chosen for screening their genome by PCR for the presence of riboflavin and folic acid genes. As a result, three isolates were selected and their production of the B2 and B9 were confirmed by HPLC. The three isolates were identified on species level by sequencing their 16S rRNA gene which showed 100% identical to strains of Pediococcus acidilactici. Thus, they were named as P. acidilactici WNYM01, P. acidilactici WNYM02, P. acidilactici WNYM03 and submitted to the Genbank database with accession numbers. They met the probiotic criteria by expressing 90-95% survival rate at pH (2.0-9.0) and bile salt up to 2% for 3 h in addition to their antimicrobial activity against gram positive and negative microorganisms. They also showed no hemolytic activity and common pattern for antibiotic susceptibility. Our three strains were tested individually or in mixture in vivo on rat colitis model compared to ulcerative group. The strains were administrated orally to rats in daily dose containing CFU 109 for 14 days then followed by induction of colitis using acetic acid then the oral administration was continued for more four days. The histology results, the anti-inflammatory and anti-oxidative stress biomarkers showed the protective role of the strains compared to the ulcerative group. As a conclusion, we introduce novel three probiotic candidates for pharmaceutical preparations and health applications.
format	article
author	Nahla M Mansour Wagiha S Elkalla Yasser M Ragab Mohamed A Ramadan
author_facet	Nahla M Mansour Wagiha S Elkalla Yasser M Ragab Mohamed A Ramadan
author_sort	Nahla M Mansour
title	Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
title_short	Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
title_full	Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
title_fullStr	Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
title_full_unstemmed	Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
title_sort	inhibition of acetic acid-induced colitis in rats by new pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/1e9b1308c06643c59cd12c4dafea6041
work_keys_str_mv	AT nahlammansour inhibitionofaceticacidinducedcolitisinratsbynewpediococcusacidilacticistrainsvitaminproducersrecoveredfromhumangutmicrobiota AT wagihaselkalla inhibitionofaceticacidinducedcolitisinratsbynewpediococcusacidilacticistrainsvitaminproducersrecoveredfromhumangutmicrobiota AT yassermragab inhibitionofaceticacidinducedcolitisinratsbynewpediococcusacidilacticistrainsvitaminproducersrecoveredfromhumangutmicrobiota AT mohamedaramadan inhibitionofaceticacidinducedcolitisinratsbynewpediococcusacidilacticistrainsvitaminproducersrecoveredfromhumangutmicrobiota
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Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.

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