3D conductive nanocomposite scaffold for bone tissue engineering

Aref Shahini,1 Mostafa Yazdimamaghani,2 Kenneth J Walker,2 Margaret A Eastman,3 Hamed Hatami-Marbini,4 Brenda J Smith,5 John L Ricci,6 Sundar V Madihally,2 Daryoosh Vashaee,1 Lobat Tayebi2,7 1School of Electrical and Computer Engineering, Helmerich Advanced Technology Research Center, 2School of Ch...

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Autores principales:	Shahini A, Yazdimamaghani M, Walker KJ, Eastman MA, Hatami-Marbini H, Smith BJ, Ricci JL, Madihally SV, Vashaee D, Tayebi L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1e9d5d250e6941b6b04f0970f7f4c8d8
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spelling	oai:doaj.org-article:1e9d5d250e6941b6b04f0970f7f4c8d82021-12-02T07:28:29Z3D conductive nanocomposite scaffold for bone tissue engineering1178-2013https://doaj.org/article/1e9d5d250e6941b6b04f0970f7f4c8d82013-12-01T00:00:00Zhttp://www.dovepress.com/3d-conductive-nanocomposite-scaffold-for-bone-tissue-engineering-a15382https://doaj.org/toc/1178-2013 Aref Shahini,1 Mostafa Yazdimamaghani,2 Kenneth J Walker,2 Margaret A Eastman,3 Hamed Hatami-Marbini,4 Brenda J Smith,5 John L Ricci,6 Sundar V Madihally,2 Daryoosh Vashaee,1 Lobat Tayebi2,7 1School of Electrical and Computer Engineering, Helmerich Advanced Technology Research Center, 2School of Chemical Engineering, 3Department of Chemistry, 4School of Mechanical and Aerospace Engineering, 5Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA; 6Department of Biomaterials and Biomimetics, New York University, New York, NY; 7School of Material Science and Engineering, Helmerich Advanced Technology Research Center, Oklahoma State University, Tulsa, OK, USA Abstract: Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli. Keywords: conductive polymers, bone scaffold, gelatin, bioactive glass nanoparticles, PEDOT:PSS, conductive scaffoldShahini AYazdimamaghani MWalker KJEastman MAHatami-Marbini HSmith BJRicci JLMadihally SVVashaee DTayebi LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 167-181 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shahini A Yazdimamaghani M Walker KJ Eastman MA Hatami-Marbini H Smith BJ Ricci JL Madihally SV Vashaee D Tayebi L 3D conductive nanocomposite scaffold for bone tissue engineering
description	Aref Shahini,1 Mostafa Yazdimamaghani,2 Kenneth J Walker,2 Margaret A Eastman,3 Hamed Hatami-Marbini,4 Brenda J Smith,5 John L Ricci,6 Sundar V Madihally,2 Daryoosh Vashaee,1 Lobat Tayebi2,7 1School of Electrical and Computer Engineering, Helmerich Advanced Technology Research Center, 2School of Chemical Engineering, 3Department of Chemistry, 4School of Mechanical and Aerospace Engineering, 5Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK, USA; 6Department of Biomaterials and Biomimetics, New York University, New York, NY; 7School of Material Science and Engineering, Helmerich Advanced Technology Research Center, Oklahoma State University, Tulsa, OK, USA Abstract: Bone healing can be significantly expedited by applying electrical stimuli in the injured region. Therefore, a three-dimensional (3D) ceramic conductive tissue engineering scaffold for large bone defects that can locally deliver the electrical stimuli is highly desired. In the present study, 3D conductive scaffolds were prepared by employing a biocompatible conductive polymer, ie, poly(3,4-ethylenedioxythiophene) poly(4-styrene sulfonate) (PEDOT:PSS), in the optimized nanocomposite of gelatin and bioactive glass. For in vitro analysis, adult human mesenchymal stem cells were seeded in the scaffolds. Material characterizations using hydrogen-1 nuclear magnetic resonance, in vitro degradation, as well as thermal and mechanical analysis showed that incorporation of PEDOT:PSS increased the physiochemical stability of the composite, resulting in improved mechanical properties and biodegradation resistance. The outcomes indicate that PEDOT:PSS and polypeptide chains have close interaction, most likely by forming salt bridges between arginine side chains and sulfonate groups. The morphology of the scaffolds and cultured human mesenchymal stem cells were observed and analyzed via scanning electron microscope, micro-computed tomography, and confocal fluorescent microscope. Increasing the concentration of the conductive polymer in the scaffold enhanced the cell viability, indicating the improved microstructure of the scaffolds or boosted electrical signaling among cells. These results show that these conductive scaffolds are not only structurally more favorable for bone tissue engineering, but also can be a step forward in combining the tissue engineering techniques with the method of enhancing the bone healing by electrical stimuli. Keywords: conductive polymers, bone scaffold, gelatin, bioactive glass nanoparticles, PEDOT:PSS, conductive scaffold
format	article
author	Shahini A Yazdimamaghani M Walker KJ Eastman MA Hatami-Marbini H Smith BJ Ricci JL Madihally SV Vashaee D Tayebi L
author_facet	Shahini A Yazdimamaghani M Walker KJ Eastman MA Hatami-Marbini H Smith BJ Ricci JL Madihally SV Vashaee D Tayebi L
author_sort	Shahini A
title	3D conductive nanocomposite scaffold for bone tissue engineering
title_short	3D conductive nanocomposite scaffold for bone tissue engineering
title_full	3D conductive nanocomposite scaffold for bone tissue engineering
title_fullStr	3D conductive nanocomposite scaffold for bone tissue engineering
title_full_unstemmed	3D conductive nanocomposite scaffold for bone tissue engineering
title_sort	3d conductive nanocomposite scaffold for bone tissue engineering
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/1e9d5d250e6941b6b04f0970f7f4c8d8
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3D conductive nanocomposite scaffold for bone tissue engineering

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