Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization

Over the past decade, cell therapy has found many applications in the treatment of different diseases. Some of the cells already used in clinical practice include stem cells and CAR-T cells. Compared with traditional drugs, living cells are much more complicated systems that must be strictly control...

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Autores principales:	Anna N. Gabashvili, Stepan S. Vodopyanov, Nelly S. Chmelyuk, Viktoria A. Sarkisova, Konstantin A. Fedotov, Maria V. Efremova, Maxim A. Abakumov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	encapsulins magnetic resonance imaging cell tracking Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/1ea72b4e71d8486494857f052dbc73b2
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spelling	oai:doaj.org-article:1ea72b4e71d8486494857f052dbc73b22021-11-25T17:54:56ZEncapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization10.3390/ijms2222122751422-00671661-6596https://doaj.org/article/1ea72b4e71d8486494857f052dbc73b22021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12275https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Over the past decade, cell therapy has found many applications in the treatment of different diseases. Some of the cells already used in clinical practice include stem cells and CAR-T cells. Compared with traditional drugs, living cells are much more complicated systems that must be strictly controlled to avoid undesirable migration, differentiation, or proliferation. One of the approaches used to prevent such side effects involves monitoring cell distribution in the human body by any noninvasive technique, such as magnetic resonance imaging (MRI). Long-term tracking of stem cells with artificial magnetic labels, such as magnetic nanoparticles, is quite problematic because such labels can affect the metabolic process and cell viability. Additionally, the concentration of exogenous labels will decrease during cell division, leading to a corresponding decrease in signal intensity. In the current work, we present a new type of genetically encoded label based on encapsulin from <i>Myxococcus xanthus</i> bacteria, stably expressed in human mesenchymal stem cells (MSCs) and coexpressed with ferroxidase as a cargo protein for nanoparticles’ synthesis inside encapsulin shells. mZip14 protein was expressed for the enhancement of iron transport into the cell. Together, these three proteins led to the synthesis of iron-containing nanoparticles in mesenchymal stem cells—without affecting cell viability—and increased contrast properties of MSCs in MRI.Anna N. GabashviliStepan S. VodopyanovNelly S. ChmelyukViktoria A. SarkisovaKonstantin A. FedotovMaria V. EfremovaMaxim A. AbakumovMDPI AGarticleencapsulinsmagnetic resonance imagingcell trackingBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12275, p 12275 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	encapsulins magnetic resonance imaging cell tracking Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	encapsulins magnetic resonance imaging cell tracking Biology (General) QH301-705.5 Chemistry QD1-999 Anna N. Gabashvili Stepan S. Vodopyanov Nelly S. Chmelyuk Viktoria A. Sarkisova Konstantin A. Fedotov Maria V. Efremova Maxim A. Abakumov Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization
description	Over the past decade, cell therapy has found many applications in the treatment of different diseases. Some of the cells already used in clinical practice include stem cells and CAR-T cells. Compared with traditional drugs, living cells are much more complicated systems that must be strictly controlled to avoid undesirable migration, differentiation, or proliferation. One of the approaches used to prevent such side effects involves monitoring cell distribution in the human body by any noninvasive technique, such as magnetic resonance imaging (MRI). Long-term tracking of stem cells with artificial magnetic labels, such as magnetic nanoparticles, is quite problematic because such labels can affect the metabolic process and cell viability. Additionally, the concentration of exogenous labels will decrease during cell division, leading to a corresponding decrease in signal intensity. In the current work, we present a new type of genetically encoded label based on encapsulin from <i>Myxococcus xanthus</i> bacteria, stably expressed in human mesenchymal stem cells (MSCs) and coexpressed with ferroxidase as a cargo protein for nanoparticles’ synthesis inside encapsulin shells. mZip14 protein was expressed for the enhancement of iron transport into the cell. Together, these three proteins led to the synthesis of iron-containing nanoparticles in mesenchymal stem cells—without affecting cell viability—and increased contrast properties of MSCs in MRI.
format	article
author	Anna N. Gabashvili Stepan S. Vodopyanov Nelly S. Chmelyuk Viktoria A. Sarkisova Konstantin A. Fedotov Maria V. Efremova Maxim A. Abakumov
author_facet	Anna N. Gabashvili Stepan S. Vodopyanov Nelly S. Chmelyuk Viktoria A. Sarkisova Konstantin A. Fedotov Maria V. Efremova Maxim A. Abakumov
author_sort	Anna N. Gabashvili
title	Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization
title_short	Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization
title_full	Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization
title_fullStr	Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization
title_full_unstemmed	Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization
title_sort	encapsulin based self-assembling iron-containing protein nanoparticles for stem cells mri visualization
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1ea72b4e71d8486494857f052dbc73b2
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Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization

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