Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization
<i>Campylobacter jejuni</i>, a prevalent foodborne bacterial pathogen, is mainly transmitted from poultry with few effective prevention approaches. In this study, we aimed to investigate the role of microbiota on <i>C. jejuni</i> chicken colonization. Microbiota from specific...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/1eb21036fec24c159ed46fdcdcc29f71 |
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| Sumario: | <i>Campylobacter jejuni</i>, a prevalent foodborne bacterial pathogen, is mainly transmitted from poultry with few effective prevention approaches. In this study, we aimed to investigate the role of microbiota on <i>C. jejuni</i> chicken colonization. Microbiota from specific pathogen-free (SPF) mouse stools were collected as SPF-Aerobe and SPF-Anaerobe. Birds were colonized with SPF-Aerobe or SPF-Anaerobe at day 0 and infected with <i>C. jejuni</i> AR101 at day 12. Notably, <i>C. jejuni</i> AR101 colonized at 5.3 and 5.6 log<sub>10</sub> <i>C. jejuni</i> CFU/g chicken cecal digesta at days 21 and 28, respectively, while both SPF-Aerobe and SPF-Anaerobe microbiota reduced pathogen colonization. Notably, SPF-Aerobe and SPF-Anaerobe increased cecal phylum <i>Bacteroidetes</i> and reduced phylum <i>Firmicutes</i> compared to those in the nontransplanted birds. Interestingly, microbiota from noninfected chickens, SPF-Aerobe, or SPF-Anaerobe inhibited AR101 in vitro growth, whereas microbiota from infected birds alone failed to reduce pathogen growth. The bacterium <i>Enterobacter</i>102 isolated from infected birds transplanted with SPF-Aerobe inhibited AR101 in vitro growth and reduced pathogen gut colonization in chickens. Together, SPF mouse microbiota was able to colonize chicken gut and reduce <i>C. jejuni</i> chicken colonization. The findings may help the development of effective strategies to reduce <i>C. jejuni</i> chicken contamination and campylobacteriosis. |
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