Microbiota from Specific Pathogen-Free Mice Reduces Campylobacter jejuni Chicken Colonization

Campylobacter jejuni, a prevalent foodborne bacterial pathogen, is mainly transmitted from poultry with few effective prevention approaches. In this study, we aimed to investigate the role of microbiota on C. jejuni chicken colonization. Microbiota from specific...

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Autores principales:	Ayidh Almansour, Ying Fu, Tahrir Alenezi, Mohit Bansal, Bilal Alrubaye, Hong Wang, Xiaolun Sun
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microbiota transplantation foodborne pathogen intestine bacterial colonization specific pathogen-free Medicine R
Acceso en línea:	https://doaj.org/article/1eb21036fec24c159ed46fdcdcc29f71
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spelling	oai:doaj.org-article:1eb21036fec24c159ed46fdcdcc29f712021-11-25T18:37:50ZMicrobiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization10.3390/pathogens101113872076-0817https://doaj.org/article/1eb21036fec24c159ed46fdcdcc29f712021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1387https://doaj.org/toc/2076-0817<i>Campylobacter jejuni</i>, a prevalent foodborne bacterial pathogen, is mainly transmitted from poultry with few effective prevention approaches. In this study, we aimed to investigate the role of microbiota on <i>C. jejuni</i> chicken colonization. Microbiota from specific pathogen-free (SPF) mouse stools were collected as SPF-Aerobe and SPF-Anaerobe. Birds were colonized with SPF-Aerobe or SPF-Anaerobe at day 0 and infected with <i>C. jejuni</i> AR101 at day 12. Notably, <i>C. jejuni</i> AR101 colonized at 5.3 and 5.6 log<sub>10</sub> <i>C. jejuni</i> CFU/g chicken cecal digesta at days 21 and 28, respectively, while both SPF-Aerobe and SPF-Anaerobe microbiota reduced pathogen colonization. Notably, SPF-Aerobe and SPF-Anaerobe increased cecal phylum <i>Bacteroidetes</i> and reduced phylum <i>Firmicutes</i> compared to those in the nontransplanted birds. Interestingly, microbiota from noninfected chickens, SPF-Aerobe, or SPF-Anaerobe inhibited AR101 in vitro growth, whereas microbiota from infected birds alone failed to reduce pathogen growth. The bacterium <i>Enterobacter</i>102 isolated from infected birds transplanted with SPF-Aerobe inhibited AR101 in vitro growth and reduced pathogen gut colonization in chickens. Together, SPF mouse microbiota was able to colonize chicken gut and reduce <i>C. jejuni</i> chicken colonization. The findings may help the development of effective strategies to reduce <i>C. jejuni</i> chicken contamination and campylobacteriosis.Ayidh AlmansourYing FuTahrir AleneziMohit BansalBilal AlrubayeHong WangXiaolun SunMDPI AGarticlemicrobiota transplantationfoodborne pathogenintestinebacterial colonizationspecific pathogen-freeMedicineRENPathogens, Vol 10, Iss 1387, p 1387 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbiota transplantation foodborne pathogen intestine bacterial colonization specific pathogen-free Medicine R
spellingShingle	microbiota transplantation foodborne pathogen intestine bacterial colonization specific pathogen-free Medicine R Ayidh Almansour Ying Fu Tahrir Alenezi Mohit Bansal Bilal Alrubaye Hong Wang Xiaolun Sun Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization
description	<i>Campylobacter jejuni</i>, a prevalent foodborne bacterial pathogen, is mainly transmitted from poultry with few effective prevention approaches. In this study, we aimed to investigate the role of microbiota on <i>C. jejuni</i> chicken colonization. Microbiota from specific pathogen-free (SPF) mouse stools were collected as SPF-Aerobe and SPF-Anaerobe. Birds were colonized with SPF-Aerobe or SPF-Anaerobe at day 0 and infected with <i>C. jejuni</i> AR101 at day 12. Notably, <i>C. jejuni</i> AR101 colonized at 5.3 and 5.6 log<sub>10</sub> <i>C. jejuni</i> CFU/g chicken cecal digesta at days 21 and 28, respectively, while both SPF-Aerobe and SPF-Anaerobe microbiota reduced pathogen colonization. Notably, SPF-Aerobe and SPF-Anaerobe increased cecal phylum <i>Bacteroidetes</i> and reduced phylum <i>Firmicutes</i> compared to those in the nontransplanted birds. Interestingly, microbiota from noninfected chickens, SPF-Aerobe, or SPF-Anaerobe inhibited AR101 in vitro growth, whereas microbiota from infected birds alone failed to reduce pathogen growth. The bacterium <i>Enterobacter</i>102 isolated from infected birds transplanted with SPF-Aerobe inhibited AR101 in vitro growth and reduced pathogen gut colonization in chickens. Together, SPF mouse microbiota was able to colonize chicken gut and reduce <i>C. jejuni</i> chicken colonization. The findings may help the development of effective strategies to reduce <i>C. jejuni</i> chicken contamination and campylobacteriosis.
format	article
author	Ayidh Almansour Ying Fu Tahrir Alenezi Mohit Bansal Bilal Alrubaye Hong Wang Xiaolun Sun
author_facet	Ayidh Almansour Ying Fu Tahrir Alenezi Mohit Bansal Bilal Alrubaye Hong Wang Xiaolun Sun
author_sort	Ayidh Almansour
title	Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization
title_short	Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization
title_full	Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization
title_fullStr	Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization
title_full_unstemmed	Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization
title_sort	microbiota from specific pathogen-free mice reduces <i>campylobacter jejuni</i> chicken colonization
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1eb21036fec24c159ed46fdcdcc29f71
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Microbiota from Specific Pathogen-Free Mice Reduces <i>Campylobacter jejuni</i> Chicken Colonization

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