RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation

Sarabjeet Singh Suri1, Steven Mills1, Gurpreet Kaur Aulakh1, Felaniaina Rakotondradany2, Hicham Fenniri2, Baljit Singh11Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon; 2National Institute for Nanotechnology and Department of Chemistry, Edmonton, CanadaAbstract: R...

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Autores principales:	Suri SS, Mills S, Aulakh GK, Rakotondradany F, Fenniri H, Singh B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1eb2b56f575a4be7b1273e38009c459a
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spelling	oai:doaj.org-article:1eb2b56f575a4be7b1273e38009c459a2021-12-02T05:40:30ZRGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation1176-91141178-2013https://doaj.org/article/1eb2b56f575a4be7b1273e38009c459a2011-12-01T00:00:00Zhttp://www.dovepress.com/rgd-tagged-helical-rosette-nanotubes-aggravate-acute-lipopolysaccharid-a8786https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sarabjeet Singh Suri1, Steven Mills1, Gurpreet Kaur Aulakh1, Felaniaina Rakotondradany2, Hicham Fenniri2, Baljit Singh11Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon; 2National Institute for Nanotechnology and Department of Chemistry, Edmonton, CanadaAbstract: Rosette nanotubes (RNT) are a novel class of self-assembled biocompatible nanotubes that offer a built-in strategy for engineering structure and function through covalent tagging of synthetic self-assembling modules (G&and;C motif). In this report, the G&and;C motif was tagged with peptide Arg-Gly-Asp-Ser-Lys (RGDSK-G&and;C) and amino acid Lys (K-G&and;C) which, upon co-assembly, generate RNTs featuring RGDSK and K on their surface in predefined molar ratios. These hybrid RNTs, referred to as Kx/RGDSKy-RNT, where x and y refer to the molar ratios of K-G&and;C and RGDSK&ndash;G&and;C, were designed to target neutrophil integrins. A mouse model was used to investigate the effects of intravenous Kx/RGDSKy-RNT on acute lipopolysaccharide (LPS)-induced lung inflammation. Healthy male C57BL/6 mice were treated intranasally with Escherichia coli LPS 80 &micro;g and/or intravenously with K90/RGDSK10-RNT. Here we provide the first evidence that intravenous administration of K90/RGDSK10-RNT aggravates the proinflammatory effect of LPS in the mouse. LPS and K90/RGDSK10-RNT treatment groups showed significantly increased infiltration of polymorphonuclear cells in bronchoalveolar lavage fluid at all time points compared with the saline control. The combined effect of LPS and K90/RGDSK10-RNT was more pronounced than LPS alone, as shown by a significant increase in the expression of interleukin-1&szlig;, MCP-1, MIP-1, and KC-1 in the bronchoalveolar lavage fluid and myeloperoxidase activity in the lung tissues. We conclude that K90/RGDSK10-RNT promotes acute lung inflammation, and when used along with LPS, leads to exaggerated immune response in the lung.Keywords: RGD peptide, helical rosette nanotubes, neutrophils, macrophages, chemokines, inflammationSuri SSMills SAulakh GKRakotondradany FFenniri HSingh BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3113-3123 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Suri SS Mills S Aulakh GK Rakotondradany F Fenniri H Singh B RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation
description	Sarabjeet Singh Suri1, Steven Mills1, Gurpreet Kaur Aulakh1, Felaniaina Rakotondradany2, Hicham Fenniri2, Baljit Singh11Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon; 2National Institute for Nanotechnology and Department of Chemistry, Edmonton, CanadaAbstract: Rosette nanotubes (RNT) are a novel class of self-assembled biocompatible nanotubes that offer a built-in strategy for engineering structure and function through covalent tagging of synthetic self-assembling modules (G&and;C motif). In this report, the G&and;C motif was tagged with peptide Arg-Gly-Asp-Ser-Lys (RGDSK-G&and;C) and amino acid Lys (K-G&and;C) which, upon co-assembly, generate RNTs featuring RGDSK and K on their surface in predefined molar ratios. These hybrid RNTs, referred to as Kx/RGDSKy-RNT, where x and y refer to the molar ratios of K-G&and;C and RGDSK&ndash;G&and;C, were designed to target neutrophil integrins. A mouse model was used to investigate the effects of intravenous Kx/RGDSKy-RNT on acute lipopolysaccharide (LPS)-induced lung inflammation. Healthy male C57BL/6 mice were treated intranasally with Escherichia coli LPS 80 &micro;g and/or intravenously with K90/RGDSK10-RNT. Here we provide the first evidence that intravenous administration of K90/RGDSK10-RNT aggravates the proinflammatory effect of LPS in the mouse. LPS and K90/RGDSK10-RNT treatment groups showed significantly increased infiltration of polymorphonuclear cells in bronchoalveolar lavage fluid at all time points compared with the saline control. The combined effect of LPS and K90/RGDSK10-RNT was more pronounced than LPS alone, as shown by a significant increase in the expression of interleukin-1&szlig;, MCP-1, MIP-1, and KC-1 in the bronchoalveolar lavage fluid and myeloperoxidase activity in the lung tissues. We conclude that K90/RGDSK10-RNT promotes acute lung inflammation, and when used along with LPS, leads to exaggerated immune response in the lung.Keywords: RGD peptide, helical rosette nanotubes, neutrophils, macrophages, chemokines, inflammation
format	article
author	Suri SS Mills S Aulakh GK Rakotondradany F Fenniri H Singh B
author_facet	Suri SS Mills S Aulakh GK Rakotondradany F Fenniri H Singh B
author_sort	Suri SS
title	RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation
title_short	RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation
title_full	RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation
title_fullStr	RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation
title_full_unstemmed	RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation
title_sort	rgd-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/1eb2b56f575a4be7b1273e38009c459a
work_keys_str_mv	AT suriss rgdtaggedhelicalrosettenanotubesaggravateacutelipopolysaccharideinducedlunginflammation AT millss rgdtaggedhelicalrosettenanotubesaggravateacutelipopolysaccharideinducedlunginflammation AT aulakhgk rgdtaggedhelicalrosettenanotubesaggravateacutelipopolysaccharideinducedlunginflammation AT rakotondradanyf rgdtaggedhelicalrosettenanotubesaggravateacutelipopolysaccharideinducedlunginflammation AT fennirih rgdtaggedhelicalrosettenanotubesaggravateacutelipopolysaccharideinducedlunginflammation AT singhb rgdtaggedhelicalrosettenanotubesaggravateacutelipopolysaccharideinducedlunginflammation
_version_	1718400280266014720

RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation

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