Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

Wakjira Kebede,1 Molalegne Bitew,2 Fufa Dawo Bari,3 Bedaso Mammo Edao,3 Hawa Mohammed,1 Martha Yami,1 Belayneh Getachew,1 Takele Abayneh,1 Esayas Gelaye1 1National Veterinary Institute, Bishoftu, Ethiopia; 2Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia; 3College of Veterinary Medicine and...

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Autores principales:	Kebede W, Bitew M, Bari FD, Edao BM, Mohammed H, Yami M, Getachew B, Abayneh T, Gelaye E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	antibody chicks ibd vaccine immunogenicity protection vero cell Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/1eb49e7c777c46acac8562362e383108
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spelling	oai:doaj.org-article:1eb49e7c777c46acac8562362e3831082021-12-02T17:36:32ZImmunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain2230-2034https://doaj.org/article/1eb49e7c777c46acac8562362e3831082021-10-01T00:00:00Zhttps://www.dovepress.com/immunogenicity-and-efficacy-evaluation-of-vero-cell-adapted-infectious-peer-reviewed-fulltext-article-VMRRhttps://doaj.org/toc/2230-2034Wakjira Kebede,1 Molalegne Bitew,2 Fufa Dawo Bari,3 Bedaso Mammo Edao,3 Hawa Mohammed,1 Martha Yami,1 Belayneh Getachew,1 Takele Abayneh,1 Esayas Gelaye1 1National Veterinary Institute, Bishoftu, Ethiopia; 2Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia; 3College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, EthiopiaCorrespondence: Esayas GelayeNational Veterinary Institute (NVI), PO Box: 19, Bishoftu, EthiopiaTel +251 923625362Fax +251 114339300Email esayasgelaye@gmail.comFufa Dawo BariCollege of Veterinary Medicine and Agriculture, Addis Ababa University, PO Box: 34, Bishoftu, EthiopiaTel +251 929190312Fax +251 114339933Email fufa.dawo@aau.edu.etIntroduction: Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease has been endemic in Ethiopia since 2002, and vaccination has been practiced as the major means of disease prevention and control. An IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell, which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells and to evaluate the safety, immunogenicity and protection level.Methods: Identity of the vaccine seed was confirmed with gene-specific primers using reverse transcription polymerase chain reaction (RT-PCR). Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage 10. A characteristic virus-induced cytopathic effect (CPE) was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. The virus-induced specific antibody was determined using indirect ELISA after vaccination of chicks through ocular route.Results: The antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine, hence no significant difference. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality.Conclusion: It is concluded that the IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibody development and successfully protects chicks against challenge with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture at the industrial scale to conquer the limitations caused by using CEF cells and thus to vaccinate chicks population to protect against the circulating IBDV infection.Keywords: antibody, chicks, IBD vaccine, immunogenicity, protection, Vero cellKebede WBitew MBari FDEdao BMMohammed HYami MGetachew BAbayneh TGelaye EDove Medical Pressarticleantibodychicksibd vaccineimmunogenicityprotectionvero cellVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol Volume 12, Pp 261-270 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibody chicks ibd vaccine immunogenicity protection vero cell Veterinary medicine SF600-1100
spellingShingle	antibody chicks ibd vaccine immunogenicity protection vero cell Veterinary medicine SF600-1100 Kebede W Bitew M Bari FD Edao BM Mohammed H Yami M Getachew B Abayneh T Gelaye E Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain
description	Wakjira Kebede,1 Molalegne Bitew,2 Fufa Dawo Bari,3 Bedaso Mammo Edao,3 Hawa Mohammed,1 Martha Yami,1 Belayneh Getachew,1 Takele Abayneh,1 Esayas Gelaye1 1National Veterinary Institute, Bishoftu, Ethiopia; 2Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia; 3College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, EthiopiaCorrespondence: Esayas GelayeNational Veterinary Institute (NVI), PO Box: 19, Bishoftu, EthiopiaTel +251 923625362Fax +251 114339300Email esayasgelaye@gmail.comFufa Dawo BariCollege of Veterinary Medicine and Agriculture, Addis Ababa University, PO Box: 34, Bishoftu, EthiopiaTel +251 929190312Fax +251 114339933Email fufa.dawo@aau.edu.etIntroduction: Infectious bursal disease virus (IBDV) is an avian viral pathogen that causes infectious bursal disease (IBD) of chickens. The disease has been endemic in Ethiopia since 2002, and vaccination has been practiced as the major means of disease prevention and control. An IBD vaccine is produced in Ethiopia using primary chicken embryo fibroblast (CEF) cell, which is time-consuming, laborious, and uneconomical. The present study was carried out to develop cell-based IBDV LC-75 vaccine using Vero cells and to evaluate the safety, immunogenicity and protection level.Methods: Identity of the vaccine seed was confirmed with gene-specific primers using reverse transcription polymerase chain reaction (RT-PCR). Confluent monolayer of Vero cells was infected with vaccine virus and serial passage continued till passage 10. A characteristic virus-induced cytopathic effect (CPE) was observed starting from passage 2 on the third day post-infection. The infectious titer of adapted virus showed a linear increment along the passage level. The virus-induced specific antibody was determined using indirect ELISA after vaccination of chicks through ocular route.Results: The antibody titer measured from Vero cells vaccinated chicks revealed similar level with the currently available CEF cell-based vaccine, hence no significant difference. Chicks vaccinated with Vero cell adapted virus showed complete protection against very virulent IBDV, while unvaccinated group had 60% morbidity and 25% mortality.Conclusion: It is concluded that the IBDV vaccine strain well adapted on Vero cells and found to be immunogenic induces antibody development and successfully protects chicks against challenge with the circulating field IBDV isolate. Hence, it is recommended to produce IBD vaccine using Vero cell culture at the industrial scale to conquer the limitations caused by using CEF cells and thus to vaccinate chicks population to protect against the circulating IBDV infection.Keywords: antibody, chicks, IBD vaccine, immunogenicity, protection, Vero cell
format	article
author	Kebede W Bitew M Bari FD Edao BM Mohammed H Yami M Getachew B Abayneh T Gelaye E
author_facet	Kebede W Bitew M Bari FD Edao BM Mohammed H Yami M Getachew B Abayneh T Gelaye E
author_sort	Kebede W
title	Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain
title_short	Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain
title_full	Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain
title_fullStr	Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain
title_full_unstemmed	Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain
title_sort	immunogenicity and efficacy evaluation of vero cell-adapted infectious bursal disease virus lc-75 vaccine strain
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/1eb49e7c777c46acac8562362e383108
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Immunogenicity and Efficacy Evaluation of Vero Cell-Adapted Infectious Bursal Disease Virus LC-75 Vaccine Strain

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