Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation

Francielli S Genier,1 Maximilian Bizanek,1 Thomas J Webster,1,2 Amit K Roy1,2 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Wenzhou Institute of Biomaterials and Engineering (WIBE), Wenzhou University, Wenzhou, People’s Republic of China Abstract: Condit...

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Autores principales:	Genier FS, Bizanek M, Webster TJ, Roy AK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	cerium oxide nanoparticles serum starvation human dermal fibroblasts. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1ec399dee90849fa9b5152020a79a907
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spelling	oai:doaj.org-article:1ec399dee90849fa9b5152020a79a9072021-12-02T02:05:00ZIncreased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation1178-2013https://doaj.org/article/1ec399dee90849fa9b5152020a79a9072018-02-01T00:00:00Zhttps://www.dovepress.com/increased-viability-of-fibroblasts-when-pretreated-with-ceria-nanopart-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Francielli S Genier,1 Maximilian Bizanek,1 Thomas J Webster,1,2 Amit K Roy1,2 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Wenzhou Institute of Biomaterials and Engineering (WIBE), Wenzhou University, Wenzhou, People’s Republic of China Abstract: Conditions of cellular stress are often the cause of cell death or dysfunction. Sustained cell stress can lead to several health complications, such as extensive inflammatory responses, tumor growth, and necrosis. To prevent disease and protect human tissue during these conditions and to avoid medication side effects, nanomaterials with unique characteristics have been applied to biological systems. This paper introduces the pretreatment in human dermal fibroblasts with cerium oxide nanoparticles during nutritional stress. For this purpose, human dermal fibroblast cells received cell culture media with concentrations of 250 µg/mL and 500 µg/mL of nano-cerium oxide before being exposed to 24, 48, and 72 hours of serum starvation. Contrast images demonstrated higher cell confluence and cell integrity in cells pretreated with ceria nanoparticles compared to untreated cells. It was confirmed by MTS assay after 72 hours of serum starvation that higher cell viability was achieved with ceria nanoparticles. The results demonstrate the potential of cerium oxide nanoparticles as protective agents during cellular starvation. Keywords: cerium oxide, nanoparticles, serum starvation, human dermal fibroblastsGenier FSBizanek MWebster TJRoy AKDove Medical Pressarticlecerium oxidenanoparticlesserum starvationhuman dermal fibroblasts.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 895-901 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerium oxide nanoparticles serum starvation human dermal fibroblasts. Medicine (General) R5-920
spellingShingle	cerium oxide nanoparticles serum starvation human dermal fibroblasts. Medicine (General) R5-920 Genier FS Bizanek M Webster TJ Roy AK Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation
description	Francielli S Genier,1 Maximilian Bizanek,1 Thomas J Webster,1,2 Amit K Roy1,2 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Wenzhou Institute of Biomaterials and Engineering (WIBE), Wenzhou University, Wenzhou, People’s Republic of China Abstract: Conditions of cellular stress are often the cause of cell death or dysfunction. Sustained cell stress can lead to several health complications, such as extensive inflammatory responses, tumor growth, and necrosis. To prevent disease and protect human tissue during these conditions and to avoid medication side effects, nanomaterials with unique characteristics have been applied to biological systems. This paper introduces the pretreatment in human dermal fibroblasts with cerium oxide nanoparticles during nutritional stress. For this purpose, human dermal fibroblast cells received cell culture media with concentrations of 250 µg/mL and 500 µg/mL of nano-cerium oxide before being exposed to 24, 48, and 72 hours of serum starvation. Contrast images demonstrated higher cell confluence and cell integrity in cells pretreated with ceria nanoparticles compared to untreated cells. It was confirmed by MTS assay after 72 hours of serum starvation that higher cell viability was achieved with ceria nanoparticles. The results demonstrate the potential of cerium oxide nanoparticles as protective agents during cellular starvation. Keywords: cerium oxide, nanoparticles, serum starvation, human dermal fibroblasts
format	article
author	Genier FS Bizanek M Webster TJ Roy AK
author_facet	Genier FS Bizanek M Webster TJ Roy AK
author_sort	Genier FS
title	Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation
title_short	Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation
title_full	Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation
title_fullStr	Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation
title_full_unstemmed	Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation
title_sort	increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/1ec399dee90849fa9b5152020a79a907
work_keys_str_mv	AT genierfs increasedviabilityoffibroblastswhenpretreatedwithceriananoparticlesduringserumdeprivation AT bizanekm increasedviabilityoffibroblastswhenpretreatedwithceriananoparticlesduringserumdeprivation AT webstertj increasedviabilityoffibroblastswhenpretreatedwithceriananoparticlesduringserumdeprivation AT royak increasedviabilityoffibroblastswhenpretreatedwithceriananoparticlesduringserumdeprivation
_version_	1718402696708358144

Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation

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