Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.

<h4>Background</h4>The present study aimed to evaluate a hypothetical Leishmania amastigote-specific protein (LiHyp1), previously identified by an immunoproteomic approach performed in Leishmania infantum, which showed homology to the super-oxygenase gene family, attempting to select a n...

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Autores principales:	Vivian T Martins, Miguel A Chávez-Fumagalli, Lourena E Costa, Adriana M C Canavaci, Adriana M C C Martins, Paula S Lage, Daniela P Lage, Mariana C Duarte, Diogo G Valadares, Rubens D M Magalhães, Tatiana G Ribeiro, Ronaldo A P Nagem, Wanderson D Darocha, Wiliam C B Régis, Manuel Soto, Eduardo A F Coelho, Ana Paula Fernandes, Carlos A P Tavares
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/1ed48caba5074aee8f85afeec8e6a389
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spelling	oai:doaj.org-article:1ed48caba5074aee8f85afeec8e6a3892021-11-18T09:15:11ZAntigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.1935-27271935-273510.1371/journal.pntd.0002148https://doaj.org/article/1ed48caba5074aee8f85afeec8e6a3892013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23573301/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>The present study aimed to evaluate a hypothetical Leishmania amastigote-specific protein (LiHyp1), previously identified by an immunoproteomic approach performed in Leishmania infantum, which showed homology to the super-oxygenase gene family, attempting to select a new candidate antigen for specific serodiagnosis, as well as to compose a vaccine against VL.<h4>Methodology/principal findings</h4>The LiHyp1 DNA sequence was cloned; the recombinant protein (rLiHyp1) was purified and evaluated for its antigenicity and immunogenicity. The rLiHyp1 protein was recognized by antibodies from sera of asymptomatic and symptomatic animals with canine visceral leishmaniasis (CVL), but presented no cross-reactivity with sera of dogs vaccinated with Leish-Tec, a Brazilian commercial vaccine; with Chagas' disease or healthy animals. In addition, the immunogenicity and protective efficacy of rLiHyp1 plus saponin was evaluated in BALB/c mice challenged subcutaneously with virulent L. infantum promastigotes. rLiHyp1 plus saponin vaccinated mice showed a high and specific production of IFN-γ, IL-12, and GM-CSF after in vitro stimulation with the recombinant protein. Immunized and infected mice, as compared to the control groups (saline and saponin), showed significant reductions in the number of parasites found in the liver, spleen, bone marrow, and in the paws' draining lymph nodes. Protection was associated with an IL-12-dependent production of IFN-γ, produced mainly by CD4 T cells. In these mice, a decrease in the parasite-mediated IL-4 and IL-10 response could also be observed.<h4>Conclusions/significance</h4>The present study showed that this Leishmania oxygenase amastigote-specific protein can be used for a more sensitive and specific serodiagnosis of asymptomatic and symptomatic CVL and, when combined with a Th1-type adjuvant, can also be employ as a candidate antigen to develop vaccines against VL.Vivian T MartinsMiguel A Chávez-FumagalliLourena E CostaAdriana M C CanavaciAdriana M C C MartinsPaula S LageDaniela P LageMariana C DuarteDiogo G ValadaresRubens D M MagalhãesTatiana G RibeiroRonaldo A P NagemWanderson D DarochaWiliam C B RégisManuel SotoEduardo A F CoelhoAna Paula FernandesCarlos A P TavaresPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2148 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vivian T Martins Miguel A Chávez-Fumagalli Lourena E Costa Adriana M C Canavaci Adriana M C C Martins Paula S Lage Daniela P Lage Mariana C Duarte Diogo G Valadares Rubens D M Magalhães Tatiana G Ribeiro Ronaldo A P Nagem Wanderson D Darocha Wiliam C B Régis Manuel Soto Eduardo A F Coelho Ana Paula Fernandes Carlos A P Tavares Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.
description	<h4>Background</h4>The present study aimed to evaluate a hypothetical Leishmania amastigote-specific protein (LiHyp1), previously identified by an immunoproteomic approach performed in Leishmania infantum, which showed homology to the super-oxygenase gene family, attempting to select a new candidate antigen for specific serodiagnosis, as well as to compose a vaccine against VL.<h4>Methodology/principal findings</h4>The LiHyp1 DNA sequence was cloned; the recombinant protein (rLiHyp1) was purified and evaluated for its antigenicity and immunogenicity. The rLiHyp1 protein was recognized by antibodies from sera of asymptomatic and symptomatic animals with canine visceral leishmaniasis (CVL), but presented no cross-reactivity with sera of dogs vaccinated with Leish-Tec, a Brazilian commercial vaccine; with Chagas' disease or healthy animals. In addition, the immunogenicity and protective efficacy of rLiHyp1 plus saponin was evaluated in BALB/c mice challenged subcutaneously with virulent L. infantum promastigotes. rLiHyp1 plus saponin vaccinated mice showed a high and specific production of IFN-γ, IL-12, and GM-CSF after in vitro stimulation with the recombinant protein. Immunized and infected mice, as compared to the control groups (saline and saponin), showed significant reductions in the number of parasites found in the liver, spleen, bone marrow, and in the paws' draining lymph nodes. Protection was associated with an IL-12-dependent production of IFN-γ, produced mainly by CD4 T cells. In these mice, a decrease in the parasite-mediated IL-4 and IL-10 response could also be observed.<h4>Conclusions/significance</h4>The present study showed that this Leishmania oxygenase amastigote-specific protein can be used for a more sensitive and specific serodiagnosis of asymptomatic and symptomatic CVL and, when combined with a Th1-type adjuvant, can also be employ as a candidate antigen to develop vaccines against VL.
format	article
author	Vivian T Martins Miguel A Chávez-Fumagalli Lourena E Costa Adriana M C Canavaci Adriana M C C Martins Paula S Lage Daniela P Lage Mariana C Duarte Diogo G Valadares Rubens D M Magalhães Tatiana G Ribeiro Ronaldo A P Nagem Wanderson D Darocha Wiliam C B Régis Manuel Soto Eduardo A F Coelho Ana Paula Fernandes Carlos A P Tavares
author_facet	Vivian T Martins Miguel A Chávez-Fumagalli Lourena E Costa Adriana M C Canavaci Adriana M C C Martins Paula S Lage Daniela P Lage Mariana C Duarte Diogo G Valadares Rubens D M Magalhães Tatiana G Ribeiro Ronaldo A P Nagem Wanderson D Darocha Wiliam C B Régis Manuel Soto Eduardo A F Coelho Ana Paula Fernandes Carlos A P Tavares
author_sort	Vivian T Martins
title	Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.
title_short	Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.
title_full	Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.
title_fullStr	Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.
title_full_unstemmed	Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.
title_sort	antigenicity and protective efficacy of a leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1ed48caba5074aee8f85afeec8e6a389
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Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis.

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