Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.

Variations in the abundance of fossil charcoals between rocks and sediments are assumed to reflect changes in fire activity in Earth's past. These variations in fire activity are often considered to be in response to environmental, ecological or climatic changes. The role that fire plays in fee...

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Autores principales:	Claire M Belcher, Surangi W Punyasena, Mayandi Sivaguru
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1eddaca6c14e456eb957fe95a3d35ba9
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spelling	oai:doaj.org-article:1eddaca6c14e456eb957fe95a3d35ba92021-11-18T08:59:24ZNovel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.1932-620310.1371/journal.pone.0072265https://doaj.org/article/1eddaca6c14e456eb957fe95a3d35ba92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977267/?tool=EBIhttps://doaj.org/toc/1932-6203Variations in the abundance of fossil charcoals between rocks and sediments are assumed to reflect changes in fire activity in Earth's past. These variations in fire activity are often considered to be in response to environmental, ecological or climatic changes. The role that fire plays in feedbacks to such changes is becoming increasingly important to understand and highlights the need to create robust estimates of variations in fossil charcoal abundance. The majority of charcoal based fire reconstructions quantify the abundance of charcoal particles and do not consider the changes in the morphology of the individual particles that may have occurred due to fragmentation as part of their transport history. We have developed a novel application of confocal laser scanning microscopy coupled to image processing that enables the 3-dimensional reconstruction of individual charcoal particles. This method is able to measure the volume of both microfossil and mesofossil charcoal particles and allows the abundance of charcoal in a sample to be expressed as total volume of charcoal. The method further measures particle surface area and shape allowing both relationships between different size and shape metrics to be analysed and full consideration of variations in particle size and size sorting between different samples to be studied. We believe application of this new imaging approach could allow significant improvement in our ability to estimate variations in past fire activity using fossil charcoals.Claire M BelcherSurangi W PunyasenaMayandi SivaguruPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72265 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire M Belcher Surangi W Punyasena Mayandi Sivaguru Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.
description	Variations in the abundance of fossil charcoals between rocks and sediments are assumed to reflect changes in fire activity in Earth's past. These variations in fire activity are often considered to be in response to environmental, ecological or climatic changes. The role that fire plays in feedbacks to such changes is becoming increasingly important to understand and highlights the need to create robust estimates of variations in fossil charcoal abundance. The majority of charcoal based fire reconstructions quantify the abundance of charcoal particles and do not consider the changes in the morphology of the individual particles that may have occurred due to fragmentation as part of their transport history. We have developed a novel application of confocal laser scanning microscopy coupled to image processing that enables the 3-dimensional reconstruction of individual charcoal particles. This method is able to measure the volume of both microfossil and mesofossil charcoal particles and allows the abundance of charcoal in a sample to be expressed as total volume of charcoal. The method further measures particle surface area and shape allowing both relationships between different size and shape metrics to be analysed and full consideration of variations in particle size and size sorting between different samples to be studied. We believe application of this new imaging approach could allow significant improvement in our ability to estimate variations in past fire activity using fossil charcoals.
format	article
author	Claire M Belcher Surangi W Punyasena Mayandi Sivaguru
author_facet	Claire M Belcher Surangi W Punyasena Mayandi Sivaguru
author_sort	Claire M Belcher
title	Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.
title_short	Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.
title_full	Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.
title_fullStr	Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.
title_full_unstemmed	Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.
title_sort	novel application of confocal laser scanning microscopy and 3d volume rendering toward improving the resolution of the fossil record of charcoal.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1eddaca6c14e456eb957fe95a3d35ba9
work_keys_str_mv	AT clairembelcher novelapplicationofconfocallaserscanningmicroscopyand3dvolumerenderingtowardimprovingtheresolutionofthefossilrecordofcharcoal AT surangiwpunyasena novelapplicationofconfocallaserscanningmicroscopyand3dvolumerenderingtowardimprovingtheresolutionofthefossilrecordofcharcoal AT mayandisivaguru novelapplicationofconfocallaserscanningmicroscopyand3dvolumerenderingtowardimprovingtheresolutionofthefossilrecordofcharcoal
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Novel application of confocal laser scanning microscopy and 3D volume rendering toward improving the resolution of the fossil record of charcoal.

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