Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments

Abstract Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed u...

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Autores principales:	Peipei Zhang, Saida Essendoubi, Julia Keenliside, Tim Reuter, Kim Stanford, Robin King, Patricia Lu, Xianqin Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/1ede7a3ce2a9466fb2e41f0176405b6d
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spelling	oai:doaj.org-article:1ede7a3ce2a9466fb2e41f0176405b6d2021-12-02T16:31:52ZGenomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments10.1038/s41538-021-00097-02396-8370https://doaj.org/article/1ede7a3ce2a9466fb2e41f0176405b6d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41538-021-00097-0https://doaj.org/toc/2396-8370Abstract Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed using five conventional subtyping methods, with most or all strains being in one subgroup. Whole-genome single nucleotide polymorphism analysis confirmed the recent ancestry of the isolates from all three sources. Most environmental isolates clustered closer with pig isolates than cattle isolates. Also, a direct link was observed between 2018-outbreak environmental isolates and isolates collected from a pig farm in 2018. The majority of pig isolates harbor only one Shiga toxin gene, stx 2a , while 70% (35/50) of the cattle isolates have both stx 1a and stx 2a . The results show some E. coli O157:H7 strains could establish persistence on pig farms and as such, pigs can be a significant source of the organism.Peipei ZhangSaida EssendoubiJulia KeenlisideTim ReuterKim StanfordRobin KingPatricia LuXianqin YangNature PortfolioarticleNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENnpj Science of Food, Vol 5, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Peipei Zhang Saida Essendoubi Julia Keenliside Tim Reuter Kim Stanford Robin King Patricia Lu Xianqin Yang Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments
description	Abstract Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed using five conventional subtyping methods, with most or all strains being in one subgroup. Whole-genome single nucleotide polymorphism analysis confirmed the recent ancestry of the isolates from all three sources. Most environmental isolates clustered closer with pig isolates than cattle isolates. Also, a direct link was observed between 2018-outbreak environmental isolates and isolates collected from a pig farm in 2018. The majority of pig isolates harbor only one Shiga toxin gene, stx 2a , while 70% (35/50) of the cattle isolates have both stx 1a and stx 2a . The results show some E. coli O157:H7 strains could establish persistence on pig farms and as such, pigs can be a significant source of the organism.
format	article
author	Peipei Zhang Saida Essendoubi Julia Keenliside Tim Reuter Kim Stanford Robin King Patricia Lu Xianqin Yang
author_facet	Peipei Zhang Saida Essendoubi Julia Keenliside Tim Reuter Kim Stanford Robin King Patricia Lu Xianqin Yang
author_sort	Peipei Zhang
title	Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments
title_short	Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments
title_full	Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments
title_fullStr	Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments
title_full_unstemmed	Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments
title_sort	genomic analysis of shiga toxin-producing escherichia coli o157:h7 from cattle and pork-production related environments
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ede7a3ce2a9466fb2e41f0176405b6d
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Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments

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