Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim

ABSTRACT: One hundred and twenty chicken samples from feces (n = 80), the carcass surface at slaughter at 2 meat chicken farms (n = 20), and retail chicken meat from 5 markets (n = 20) collected during 2018 and 2019 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) in...

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Autores principales:	Toshiyuki Murase, Patchara Phuektes, Hiroichi Ozaki, Sunpetch Angkititrakul
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	chicken Escherichia coli plasmid-mediated quinolone resistance qnrS trimethoprim Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/1eec35125749487f9ad331b7e3c59d73
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spelling	oai:doaj.org-article:1eec35125749487f9ad331b7e3c59d732021-11-16T04:09:05ZPrevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim0032-579110.1016/j.psj.2021.101538https://doaj.org/article/1eec35125749487f9ad331b7e3c59d732022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005605https://doaj.org/toc/0032-5791ABSTRACT: One hundred and twenty chicken samples from feces (n = 80), the carcass surface at slaughter at 2 meat chicken farms (n = 20), and retail chicken meat from 5 markets (n = 20) collected during 2018 and 2019 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) in Escherichia coli. We detected qnrS-positive E. coli in a total of 74 samples from feces (n = 59), the carcass surface (n = 7), and retail meat (n = 8). These 74 qnrS-positive isolates were tested for antimicrobial susceptibility to determine the minimum inhibitory concentrations (MICs) of certain antimicrobials and genetically characterized. Ampicillin-resistance accounted for 71 of the 74 isolates (96%), followed by resistance to oxytetracycline (57/74; 77%), enrofloxacin (ERFX) (56/74; 76%), sulfisoxazole (SUL) (56/74; 76%), trimethoprim (TMP) (49/74; 66%), and dihydrostreptomycin (48/74; 65%). All farm-borne SUL- and TMP-resistant isolates except one were obtained from samples from farm A where a combination of sulfadiazine and TMP was administered to the chickens. Concentrations of ERFX at which 50 and 90% of isolates were inhibited were 2 μg/mL and 32 μg/mL, respectively. Diverse pulsed-field gel electrophoresis (PFGE) patterns of XbaI-digested genomic DNA were observed in the qnrS-positive isolates from fecal samples. Several isolates from feces and the carcass surface had identical XbaI-digested PFGE patterns. S1-nuclease PFGE and Southern blot analysis demonstrated that 7 of 11 dfrA13-positive fecal isolates carried both the qnrS and dfrA13 genes on the same plasmid, and 2 of 3 dfrA1-positive isolates similarly carried both qnrS and dfrA1 on the same plasmid, although the PFGE patterns of XbaI-digested genomic DNA of the isolates were different. These results suggest that the qnrS gene is prevalent in chicken farms via horizontal transfer of plasmids and may partly be co-selected under the use of TMP.Toshiyuki MurasePatchara PhuektesHiroichi OzakiSunpetch AngkititrakulElsevierarticlechickenEscherichia coliplasmid-mediated quinolone resistanceqnrStrimethoprimAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101538- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	chicken Escherichia coli plasmid-mediated quinolone resistance qnrS trimethoprim Animal culture SF1-1100
spellingShingle	chicken Escherichia coli plasmid-mediated quinolone resistance qnrS trimethoprim Animal culture SF1-1100 Toshiyuki Murase Patchara Phuektes Hiroichi Ozaki Sunpetch Angkititrakul Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim
description	ABSTRACT: One hundred and twenty chicken samples from feces (n = 80), the carcass surface at slaughter at 2 meat chicken farms (n = 20), and retail chicken meat from 5 markets (n = 20) collected during 2018 and 2019 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) in Escherichia coli. We detected qnrS-positive E. coli in a total of 74 samples from feces (n = 59), the carcass surface (n = 7), and retail meat (n = 8). These 74 qnrS-positive isolates were tested for antimicrobial susceptibility to determine the minimum inhibitory concentrations (MICs) of certain antimicrobials and genetically characterized. Ampicillin-resistance accounted for 71 of the 74 isolates (96%), followed by resistance to oxytetracycline (57/74; 77%), enrofloxacin (ERFX) (56/74; 76%), sulfisoxazole (SUL) (56/74; 76%), trimethoprim (TMP) (49/74; 66%), and dihydrostreptomycin (48/74; 65%). All farm-borne SUL- and TMP-resistant isolates except one were obtained from samples from farm A where a combination of sulfadiazine and TMP was administered to the chickens. Concentrations of ERFX at which 50 and 90% of isolates were inhibited were 2 μg/mL and 32 μg/mL, respectively. Diverse pulsed-field gel electrophoresis (PFGE) patterns of XbaI-digested genomic DNA were observed in the qnrS-positive isolates from fecal samples. Several isolates from feces and the carcass surface had identical XbaI-digested PFGE patterns. S1-nuclease PFGE and Southern blot analysis demonstrated that 7 of 11 dfrA13-positive fecal isolates carried both the qnrS and dfrA13 genes on the same plasmid, and 2 of 3 dfrA1-positive isolates similarly carried both qnrS and dfrA1 on the same plasmid, although the PFGE patterns of XbaI-digested genomic DNA of the isolates were different. These results suggest that the qnrS gene is prevalent in chicken farms via horizontal transfer of plasmids and may partly be co-selected under the use of TMP.
format	article
author	Toshiyuki Murase Patchara Phuektes Hiroichi Ozaki Sunpetch Angkititrakul
author_facet	Toshiyuki Murase Patchara Phuektes Hiroichi Ozaki Sunpetch Angkititrakul
author_sort	Toshiyuki Murase
title	Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim
title_short	Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim
title_full	Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim
title_fullStr	Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim
title_full_unstemmed	Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim
title_sort	prevalence of qnrs-positive escherichia coli from chicken in thailand and possible co-selection of isolates with plasmids carrying qnrs and trimethoprim-resistance genes under farm use of trimethoprim
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/1eec35125749487f9ad331b7e3c59d73
work_keys_str_mv	AT toshiyukimurase prevalenceofqnrspositiveescherichiacolifromchickeninthailandandpossiblecoselectionofisolateswithplasmidscarryingqnrsandtrimethoprimresistancegenesunderfarmuseoftrimethoprim AT patcharaphuektes prevalenceofqnrspositiveescherichiacolifromchickeninthailandandpossiblecoselectionofisolateswithplasmidscarryingqnrsandtrimethoprimresistancegenesunderfarmuseoftrimethoprim AT hiroichiozaki prevalenceofqnrspositiveescherichiacolifromchickeninthailandandpossiblecoselectionofisolateswithplasmidscarryingqnrsandtrimethoprimresistancegenesunderfarmuseoftrimethoprim AT sunpetchangkititrakul prevalenceofqnrspositiveescherichiacolifromchickeninthailandandpossiblecoselectionofisolateswithplasmidscarryingqnrsandtrimethoprimresistancegenesunderfarmuseoftrimethoprim
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Prevalence of qnrS-positive Escherichia coli from chicken in Thailand and possible co-selection of isolates with plasmids carrying qnrS and trimethoprim-resistance genes under farm use of trimethoprim

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