Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway

Summary Yarrowia lipolytica is widely used as a microbial producer of lipids and lipid derivatives. Here, we exploited this yeast’s potential to generate aromatic amino acids by developing chassis strains optimized for the production of phenylalanine, tyrosine and tryptophan. We engineered the shiki...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Macarena Larroude, Jean‐Marc Nicaud, Tristan Rossignol
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/1ef1f38c80f84c65a9b53173b0f16ac4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1ef1f38c80f84c65a9b53173b0f16ac4
record_format	dspace
spelling	oai:doaj.org-article:1ef1f38c80f84c65a9b53173b0f16ac42021-11-18T15:39:52ZYarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway1751-791510.1111/1751-7915.13745https://doaj.org/article/1ef1f38c80f84c65a9b53173b0f16ac42021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13745https://doaj.org/toc/1751-7915Summary Yarrowia lipolytica is widely used as a microbial producer of lipids and lipid derivatives. Here, we exploited this yeast’s potential to generate aromatic amino acids by developing chassis strains optimized for the production of phenylalanine, tyrosine and tryptophan. We engineered the shikimate pathway to overexpress a combination of Y. lipolytica and heterologous feedback‐insensitive enzyme variants. Our best chassis strain displayed high levels of de novo Ehrlich metabolite production (up to 0.14 g l−1 in minimal growth medium), which represented a 93‐fold increase compared to the wild‐type strain (0.0015 g l−1). Production was further boosted to 0.48 g l−1 when glycerol, a low‐cost carbon source, was used, concomitantly to high secretion of phenylalanine precursor (1 g l−1). Among these metabolites, 2‐phenylethanol is of particular interest due to its rose‐like flavour. We also established a production pathway for generating protodeoxyviolaceinic acid, a dye derived from tryptophan, in a chassis strain optimized for chorismate, the precursor of tryptophan. We have thus demonstrated that Y. lipolytica can serve as a platform for the sustainable de novo bio‐production of high‐value aromatic compounds, and we have greatly improved our understanding of the potential feedback‐based regulation of the shikimate pathway in this yeast.Macarena LarroudeJean‐Marc NicaudTristan RossignolWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2420-2434 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Macarena Larroude Jean‐Marc Nicaud Tristan Rossignol Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway
description	Summary Yarrowia lipolytica is widely used as a microbial producer of lipids and lipid derivatives. Here, we exploited this yeast’s potential to generate aromatic amino acids by developing chassis strains optimized for the production of phenylalanine, tyrosine and tryptophan. We engineered the shikimate pathway to overexpress a combination of Y. lipolytica and heterologous feedback‐insensitive enzyme variants. Our best chassis strain displayed high levels of de novo Ehrlich metabolite production (up to 0.14 g l−1 in minimal growth medium), which represented a 93‐fold increase compared to the wild‐type strain (0.0015 g l−1). Production was further boosted to 0.48 g l−1 when glycerol, a low‐cost carbon source, was used, concomitantly to high secretion of phenylalanine precursor (1 g l−1). Among these metabolites, 2‐phenylethanol is of particular interest due to its rose‐like flavour. We also established a production pathway for generating protodeoxyviolaceinic acid, a dye derived from tryptophan, in a chassis strain optimized for chorismate, the precursor of tryptophan. We have thus demonstrated that Y. lipolytica can serve as a platform for the sustainable de novo bio‐production of high‐value aromatic compounds, and we have greatly improved our understanding of the potential feedback‐based regulation of the shikimate pathway in this yeast.
format	article
author	Macarena Larroude Jean‐Marc Nicaud Tristan Rossignol
author_facet	Macarena Larroude Jean‐Marc Nicaud Tristan Rossignol
author_sort	Macarena Larroude
title	Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway
title_short	Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway
title_full	Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway
title_fullStr	Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway
title_full_unstemmed	Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway
title_sort	yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/1ef1f38c80f84c65a9b53173b0f16ac4
work_keys_str_mv	AT macarenalarroude yarrowialipolyticachassisstrainsengineeredtoproducearomaticaminoacidsviatheshikimatepathway AT jeanmarcnicaud yarrowialipolyticachassisstrainsengineeredtoproducearomaticaminoacidsviatheshikimatepathway AT tristanrossignol yarrowialipolyticachassisstrainsengineeredtoproducearomaticaminoacidsviatheshikimatepathway
_version_	1718420772565811200

Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway

Ejemplares similares