Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria

Abstract There are currently no preventative options for recurrent aphthous stomatitis, and the only available treatments are palliative. This is partly due to a poor understanding of its etiopathogenesis. In this case–control study, we characterized the salivary proteome of patients with recurrent...

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Autores principales:	Romina Hernández-Olivos, Mariagrazia Muñoz, Esteban Núñez, Paola Andrea Camargo-Ayala, Jenaro Garcia-Huidobro, Alfredo Pereira, Fabiane M. Nachtigall, Leonardo S. Santos, César Rivera
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ef2a71248f04b5a824805e62f14a1bc
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spelling	oai:doaj.org-article:1ef2a71248f04b5a824805e62f14a1bc2021-12-02T14:53:41ZSalivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria10.1038/s41598-021-95228-82045-2322https://doaj.org/article/1ef2a71248f04b5a824805e62f14a1bc2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95228-8https://doaj.org/toc/2045-2322Abstract There are currently no preventative options for recurrent aphthous stomatitis, and the only available treatments are palliative. This is partly due to a poor understanding of its etiopathogenesis. In this case–control study, we characterized the salivary proteome of patients with recurrent aphthous stomatitis in the presence and absence of lesions. Through mass spectrometry-based proteomics and bioinformatics tools, we identified that the presence of oral ulcers is associated with several specific biological processes, including the metabolic pathways of vitamin B9, B12, nitrogen, selenium, and the bacterium Neisseria meningitidis. These changes occurred only in the presence of clinically visible lesions, and there were no relevant differences between patients in anatomical regions unaffected by ulcers. Additionally, using western blot and ELISA assays, we verified that carbonic anhydrase 1 (CA1) and hemoglobin subunit beta (HBB) proteins are highly expressed during the ulcerative and remission phases of recurrent aphthous stomatitis. Our results cumulatively support saliva as an indicator of the pathophysiological changes, which occur during the clinical course of lesions. From a clinical perspective, we suggest that recurrent aphthous stomatitis is a condition triggered by temporary biological changes in people with lesions.Romina Hernández-OlivosMariagrazia MuñozEsteban NúñezPaola Andrea Camargo-AyalaJenaro Garcia-HuidobroAlfredo PereiraFabiane M. NachtigallLeonardo S. SantosCésar RiveraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Romina Hernández-Olivos Mariagrazia Muñoz Esteban Núñez Paola Andrea Camargo-Ayala Jenaro Garcia-Huidobro Alfredo Pereira Fabiane M. Nachtigall Leonardo S. Santos César Rivera Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria
description	Abstract There are currently no preventative options for recurrent aphthous stomatitis, and the only available treatments are palliative. This is partly due to a poor understanding of its etiopathogenesis. In this case–control study, we characterized the salivary proteome of patients with recurrent aphthous stomatitis in the presence and absence of lesions. Through mass spectrometry-based proteomics and bioinformatics tools, we identified that the presence of oral ulcers is associated with several specific biological processes, including the metabolic pathways of vitamin B9, B12, nitrogen, selenium, and the bacterium Neisseria meningitidis. These changes occurred only in the presence of clinically visible lesions, and there were no relevant differences between patients in anatomical regions unaffected by ulcers. Additionally, using western blot and ELISA assays, we verified that carbonic anhydrase 1 (CA1) and hemoglobin subunit beta (HBB) proteins are highly expressed during the ulcerative and remission phases of recurrent aphthous stomatitis. Our results cumulatively support saliva as an indicator of the pathophysiological changes, which occur during the clinical course of lesions. From a clinical perspective, we suggest that recurrent aphthous stomatitis is a condition triggered by temporary biological changes in people with lesions.
format	article
author	Romina Hernández-Olivos Mariagrazia Muñoz Esteban Núñez Paola Andrea Camargo-Ayala Jenaro Garcia-Huidobro Alfredo Pereira Fabiane M. Nachtigall Leonardo S. Santos César Rivera
author_facet	Romina Hernández-Olivos Mariagrazia Muñoz Esteban Núñez Paola Andrea Camargo-Ayala Jenaro Garcia-Huidobro Alfredo Pereira Fabiane M. Nachtigall Leonardo S. Santos César Rivera
author_sort	Romina Hernández-Olivos
title	Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria
title_short	Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria
title_full	Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria
title_fullStr	Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria
title_full_unstemmed	Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria
title_sort	salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ef2a71248f04b5a824805e62f14a1bc
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Salivary proteome of aphthous stomatitis reveals the participation of vitamin metabolism, nutrients, and bacteria

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