New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

Abstract We characterized the tissue repair response after penetrating traumatic brain injury (pTBI) in this study. Seventy specific pathogen-free Kunming mice were randomly divided into the following groups: normal control, 1, 3, 7, 15, 21, and 30 days after pTBI. Hematoxylin and eosin (H&E) st...

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Autores principales:	Fan-Wei Meng, Jun-Tao Yu, Jin-Yuan Chen, Peng-Fei Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1ef39e7a61e34eeebf26adec9709b64f
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spelling	oai:doaj.org-article:1ef39e7a61e34eeebf26adec9709b64f2021-12-02T16:50:22ZNew lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury10.1038/s41598-021-89616-32045-2322https://doaj.org/article/1ef39e7a61e34eeebf26adec9709b64f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89616-3https://doaj.org/toc/2045-2322Abstract We characterized the tissue repair response after penetrating traumatic brain injury (pTBI) in this study. Seventy specific pathogen-free Kunming mice were randomly divided into the following groups: normal control, 1, 3, 7, 15, 21, and 30 days after pTBI. Hematoxylin and eosin (H&E) staining, immunohistochemistry, and immunofluorescence were performed to examine and monitor brain tissue morphology, and the distribution and expression of lymphatic-specific markers lymphatic vessel endothelial receptor-1 (LYVE-1), hematopoietic precursor cluster of differentiation 34 (CD34) antigen, and Prospero-related homeobox-1 (PROX1) protein. H&E staining revealed that damaged and necrotic tissues observed on day 1 at and around the injury site disappeared on day 7, and there was gradual shrinkage and disappearance of the lesion on day 30, suggesting a clearance mechanism. We explored the possibility of lymphangiogenesis causing this clearance as part of the post-injury response. Notably, expression of lymphangiogenesis markers LYVE-1, CD34, and PROX1 was detected in damaged mouse brain tissue but not in normal tissue. Moreover, new lymphatic cells and colocalization of LYVE-1/CD34 and LYVE-1/PROX1 were also observed. Our findings of the formation of new lymphatic cells following pTBI provide preliminary insights into a post-injury clearance mechanism in the brain. Although we showed that lymphatic cells are implicated in brain tissue repair, further research is required to clarify the origin of these cells.Fan-Wei MengJun-Tao YuJin-Yuan ChenPeng-Fei YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fan-Wei Meng Jun-Tao Yu Jin-Yuan Chen Peng-Fei Yang New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury
description	Abstract We characterized the tissue repair response after penetrating traumatic brain injury (pTBI) in this study. Seventy specific pathogen-free Kunming mice were randomly divided into the following groups: normal control, 1, 3, 7, 15, 21, and 30 days after pTBI. Hematoxylin and eosin (H&E) staining, immunohistochemistry, and immunofluorescence were performed to examine and monitor brain tissue morphology, and the distribution and expression of lymphatic-specific markers lymphatic vessel endothelial receptor-1 (LYVE-1), hematopoietic precursor cluster of differentiation 34 (CD34) antigen, and Prospero-related homeobox-1 (PROX1) protein. H&E staining revealed that damaged and necrotic tissues observed on day 1 at and around the injury site disappeared on day 7, and there was gradual shrinkage and disappearance of the lesion on day 30, suggesting a clearance mechanism. We explored the possibility of lymphangiogenesis causing this clearance as part of the post-injury response. Notably, expression of lymphangiogenesis markers LYVE-1, CD34, and PROX1 was detected in damaged mouse brain tissue but not in normal tissue. Moreover, new lymphatic cells and colocalization of LYVE-1/CD34 and LYVE-1/PROX1 were also observed. Our findings of the formation of new lymphatic cells following pTBI provide preliminary insights into a post-injury clearance mechanism in the brain. Although we showed that lymphatic cells are implicated in brain tissue repair, further research is required to clarify the origin of these cells.
format	article
author	Fan-Wei Meng Jun-Tao Yu Jin-Yuan Chen Peng-Fei Yang
author_facet	Fan-Wei Meng Jun-Tao Yu Jin-Yuan Chen Peng-Fei Yang
author_sort	Fan-Wei Meng
title	New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury
title_short	New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury
title_full	New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury
title_fullStr	New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury
title_full_unstemmed	New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury
title_sort	new lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1ef39e7a61e34eeebf26adec9709b64f
work_keys_str_mv	AT fanweimeng newlymphaticcellformationisassociatedwithdamagedbraintissueclearanceafterpenetratingtraumaticbraininjury AT juntaoyu newlymphaticcellformationisassociatedwithdamagedbraintissueclearanceafterpenetratingtraumaticbraininjury AT jinyuanchen newlymphaticcellformationisassociatedwithdamagedbraintissueclearanceafterpenetratingtraumaticbraininjury AT pengfeiyang newlymphaticcellformationisassociatedwithdamagedbraintissueclearanceafterpenetratingtraumaticbraininjury
_version_	1718383023087419392

New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

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