Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories

ABSTRACT Diffusion microchamber array (DMA) devices have provided contemporary microbiologists with a successful alternative to the century-old plating method using petri dishes to isolate and cultivate environmental bacteria. The “ichip” DMA device, developed by the research group of Slava Epstein,...

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Autores principales:	Michael J. Braus, Robert Swader, George Petry, Thea Whitman
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	diffusion microchamber array ichip microbial cultivation microbial isolation nonmodel microorganisms soil microhabitats Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1ef434546462474894125ca699172250
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spelling	oai:doaj.org-article:1ef434546462474894125ca6991722502021-11-15T15:04:52ZAffordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories10.1128/jmbe.00131-211935-78851935-7877https://doaj.org/article/1ef434546462474894125ca6991722502021-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.00131-21https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885ABSTRACT Diffusion microchamber array (DMA) devices have provided contemporary microbiologists with a successful alternative to the century-old plating method using petri dishes to isolate and cultivate environmental bacteria. The “ichip” DMA device, developed by the research group of Slava Epstein, is exemplary of this technology, using membranes to culture rare or otherwise nonmodel bacterial taxa, whereby the target cells are nourished by environmental medium across the separating membrane. However, DMA devices have not sufficiently infiltrated the university-level microbiology curriculum, and the size and shape of the ichip make the device challenging for use by students. The following work provides guidance in the creation and use of a suite of DMA devices called “iplates,” which are ichip-like tools for use in microbiology education. An iplate mimics the shape and size of a 96-well plate, making it a simpler, more affordable, and customizable design for the isolation and incubation of bacteria and other microorganisms using fresh environmental media. Iplates and their many customizations are intended to enable teachers, students, and researchers to isolate, grow, and analyze the widest possible diversity of microorganisms, complementing standard plating methods. We offer additional tips and tools to help instructors and students get started creating DMA devices to meet their unique needs and research interests.Michael J. BrausRobert SwaderGeorge PetryThea WhitmanAmerican Society for Microbiologyarticlediffusion microchamber arrayichipmicrobial cultivationmicrobial isolationnonmodel microorganismssoil microhabitatsSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diffusion microchamber array ichip microbial cultivation microbial isolation nonmodel microorganisms soil microhabitats Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	diffusion microchamber array ichip microbial cultivation microbial isolation nonmodel microorganisms soil microhabitats Special aspects of education LC8-6691 Biology (General) QH301-705.5 Michael J. Braus Robert Swader George Petry Thea Whitman Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories
description	ABSTRACT Diffusion microchamber array (DMA) devices have provided contemporary microbiologists with a successful alternative to the century-old plating method using petri dishes to isolate and cultivate environmental bacteria. The “ichip” DMA device, developed by the research group of Slava Epstein, is exemplary of this technology, using membranes to culture rare or otherwise nonmodel bacterial taxa, whereby the target cells are nourished by environmental medium across the separating membrane. However, DMA devices have not sufficiently infiltrated the university-level microbiology curriculum, and the size and shape of the ichip make the device challenging for use by students. The following work provides guidance in the creation and use of a suite of DMA devices called “iplates,” which are ichip-like tools for use in microbiology education. An iplate mimics the shape and size of a 96-well plate, making it a simpler, more affordable, and customizable design for the isolation and incubation of bacteria and other microorganisms using fresh environmental media. Iplates and their many customizations are intended to enable teachers, students, and researchers to isolate, grow, and analyze the widest possible diversity of microorganisms, complementing standard plating methods. We offer additional tips and tools to help instructors and students get started creating DMA devices to meet their unique needs and research interests.
format	article
author	Michael J. Braus Robert Swader George Petry Thea Whitman
author_facet	Michael J. Braus Robert Swader George Petry Thea Whitman
author_sort	Michael J. Braus
title	Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories
title_short	Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories
title_full	Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories
title_fullStr	Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories
title_full_unstemmed	Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories
title_sort	affordable diffusion microchamber array designs for isolating microbes in classrooms and laboratories
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/1ef434546462474894125ca699172250
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Affordable Diffusion Microchamber Array Designs for Isolating Microbes in Classrooms and Laboratories

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