Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor

Fumonisin B1 (FB1) is a common mycotoxin contamination in agricultural commodities being considered as a significant risk to human and livestock health, while the mechanism of FB1 immunotoxicity are less understood, especially in chicken. Given that extracellular traps as a novel defense mechanism o...

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Autores principales:	Zhikai Wu, Xingyi Zhu, Peixuan Li, Xia Wang, Youpeng Sun, Yiwu Fu, Jingjing Wang, Zhengtao Yang, Ershun Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	fumonisin B1 chicken heterophil extracellular traps immunotoxicity Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/1ef745f4a1c744edb28c62b169c101ab
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spelling	oai:doaj.org-article:1ef745f4a1c744edb28c62b169c101ab2021-11-24T04:23:17ZFumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor0032-579110.1016/j.psj.2021.101550https://doaj.org/article/1ef745f4a1c744edb28c62b169c101ab2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005721https://doaj.org/toc/0032-5791Fumonisin B1 (FB1) is a common mycotoxin contamination in agricultural commodities being considered as a significant risk to human and livestock health, while the mechanism of FB1 immunotoxicity are less understood, especially in chicken. Given that extracellular traps as a novel defense mechanism of leukocytes play an important role against foreign matters, in this study we aimed to investigate the effects of FB1 on chicken heterophil extracellular traps (HETs) formation. Our result showed that FB1 induced HETs release in chicken heterophils observed via immunostaining, and it was concentration-dependent during 10 to 40 μM. Moreover, in 40 μM FB1-exposed chicken heterophils, reactive oxygen species (ROS) level was increased, while catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity and glutathione (GSH) content were decreased. Simultaneously, FB1 (40 μM) activated ERK and p38 MAPK signaling pathways via increasing the phosphorylation level of ERK and p38 proteins. However, pretreatment of SB202190, U0126, and diphenyleneiodonium chloride (DPI) did not change FB1-triggered ROS production and HETs formation, suggesting FB1-induced HETs was a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, p38, and extracellular regulated protein kinases (ERK) signaling pathways-independent process. Inhibition of peptidyl arginine deiminase 4 (PAD4) enzyme and P2 × 1 receptor showed their vital role in 40 μM FB1-triggered HETs. This study reported for the first time that 40 μM FB1 induced the release of HETs in heterophils, and it was related to ROS production, PAD4, and P2 × 1, but was independent of NADPH oxidase, p38 and ERK signaling pathways, which might provide a whole novel perspective of perceiving and understanding the role of FB1 in immunotoxicity.Zhikai WuXingyi ZhuPeixuan LiXia WangYoupeng SunYiwu FuJingjing WangZhengtao YangErshun ZhouElsevierarticlefumonisin B1chickenheterophil extracellular trapsimmunotoxicityAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101550- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	fumonisin B1 chicken heterophil extracellular traps immunotoxicity Animal culture SF1-1100
spellingShingle	fumonisin B1 chicken heterophil extracellular traps immunotoxicity Animal culture SF1-1100 Zhikai Wu Xingyi Zhu Peixuan Li Xia Wang Youpeng Sun Yiwu Fu Jingjing Wang Zhengtao Yang Ershun Zhou Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor
description	Fumonisin B1 (FB1) is a common mycotoxin contamination in agricultural commodities being considered as a significant risk to human and livestock health, while the mechanism of FB1 immunotoxicity are less understood, especially in chicken. Given that extracellular traps as a novel defense mechanism of leukocytes play an important role against foreign matters, in this study we aimed to investigate the effects of FB1 on chicken heterophil extracellular traps (HETs) formation. Our result showed that FB1 induced HETs release in chicken heterophils observed via immunostaining, and it was concentration-dependent during 10 to 40 μM. Moreover, in 40 μM FB1-exposed chicken heterophils, reactive oxygen species (ROS) level was increased, while catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activity and glutathione (GSH) content were decreased. Simultaneously, FB1 (40 μM) activated ERK and p38 MAPK signaling pathways via increasing the phosphorylation level of ERK and p38 proteins. However, pretreatment of SB202190, U0126, and diphenyleneiodonium chloride (DPI) did not change FB1-triggered ROS production and HETs formation, suggesting FB1-induced HETs was a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, p38, and extracellular regulated protein kinases (ERK) signaling pathways-independent process. Inhibition of peptidyl arginine deiminase 4 (PAD4) enzyme and P2 × 1 receptor showed their vital role in 40 μM FB1-triggered HETs. This study reported for the first time that 40 μM FB1 induced the release of HETs in heterophils, and it was related to ROS production, PAD4, and P2 × 1, but was independent of NADPH oxidase, p38 and ERK signaling pathways, which might provide a whole novel perspective of perceiving and understanding the role of FB1 in immunotoxicity.
format	article
author	Zhikai Wu Xingyi Zhu Peixuan Li Xia Wang Youpeng Sun Yiwu Fu Jingjing Wang Zhengtao Yang Ershun Zhou
author_facet	Zhikai Wu Xingyi Zhu Peixuan Li Xia Wang Youpeng Sun Yiwu Fu Jingjing Wang Zhengtao Yang Ershun Zhou
author_sort	Zhikai Wu
title	Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor
title_short	Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor
title_full	Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor
title_fullStr	Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor
title_full_unstemmed	Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor
title_sort	fumonisin b1 induces chicken heterophil extracellular traps mediated by pad4 enzyme and p2 × 1 receptor
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/1ef745f4a1c744edb28c62b169c101ab
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Fumonisin B1 induces chicken heterophil extracellular traps mediated by PAD4 enzyme and P2 × 1 receptor

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