Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein

Abstract Most cancer cells have telomerase activity because they can express the human telomerase reverse transcriptase (hTERT) gene. Therefore, the inhibition of the hTERT expression can play an important role in controlling cancer cell proliferation. Our current study aims to inhibit hTERT express...

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Autores principales:	Pankaj Attri, Jitender Gaur, Sooho Choi, Minsup Kim, Rohit Bhatia, Naresh Kumar, Ji Hoon Park, Art. E. Cho, Eun Ha Choi, Weontae Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1efb67bb46e745999fd5b85bddaf75eb
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spelling	oai:doaj.org-article:1efb67bb46e745999fd5b85bddaf75eb2021-12-02T15:04:58ZInteraction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein10.1038/s41598-017-02690-42045-2322https://doaj.org/article/1efb67bb46e745999fd5b85bddaf75eb2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02690-4https://doaj.org/toc/2045-2322Abstract Most cancer cells have telomerase activity because they can express the human telomerase reverse transcriptase (hTERT) gene. Therefore, the inhibition of the hTERT expression can play an important role in controlling cancer cell proliferation. Our current study aims to inhibit hTERT expression. For this, we synthesized graphene oxide (GO) and a functionalized multiwall carbon nanotube (f-MWCNT), latter treated them with cold atmospheric pressure plasma for further analysis of the hTERT expression. The inhibition of hTERT expression by GO, f-MWCNT, plasma activated GO solution (PGOS), and plasma activated f-MWCNT solution (PCNTS), was studied using two lung cancer cell lines, A549 and H460. The hTERT experimental results revealed that GO and PGOS sufficiently decreased the hTERT concentration, while f-MWCNT and PCNTS were unable to inhibit the hTERT concentration. Therefore, to understand the inhibition mechanism of hTERT, we studied the binding properties of GO and PGOS with telomere binding protein (AtTRB2). The interaction studies were carried out using circular dichroism, fluorescence, 1H-15N NMR spectroscopy, and size-exclusion chromatography (SEC) binding assay. We also used docking simulation to have an better understanding of the interactions between GO nanosheets and AtTRB2 protein. Our results may provide new insights that can benefit in biomedical treatments.Pankaj AttriJitender GaurSooho ChoiMinsup KimRohit BhatiaNaresh KumarJi Hoon ParkArt. E. ChoEun Ha ChoiWeontae LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pankaj Attri Jitender Gaur Sooho Choi Minsup Kim Rohit Bhatia Naresh Kumar Ji Hoon Park Art. E. Cho Eun Ha Choi Weontae Lee Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
description	Abstract Most cancer cells have telomerase activity because they can express the human telomerase reverse transcriptase (hTERT) gene. Therefore, the inhibition of the hTERT expression can play an important role in controlling cancer cell proliferation. Our current study aims to inhibit hTERT expression. For this, we synthesized graphene oxide (GO) and a functionalized multiwall carbon nanotube (f-MWCNT), latter treated them with cold atmospheric pressure plasma for further analysis of the hTERT expression. The inhibition of hTERT expression by GO, f-MWCNT, plasma activated GO solution (PGOS), and plasma activated f-MWCNT solution (PCNTS), was studied using two lung cancer cell lines, A549 and H460. The hTERT experimental results revealed that GO and PGOS sufficiently decreased the hTERT concentration, while f-MWCNT and PCNTS were unable to inhibit the hTERT concentration. Therefore, to understand the inhibition mechanism of hTERT, we studied the binding properties of GO and PGOS with telomere binding protein (AtTRB2). The interaction studies were carried out using circular dichroism, fluorescence, 1H-15N NMR spectroscopy, and size-exclusion chromatography (SEC) binding assay. We also used docking simulation to have an better understanding of the interactions between GO nanosheets and AtTRB2 protein. Our results may provide new insights that can benefit in biomedical treatments.
format	article
author	Pankaj Attri Jitender Gaur Sooho Choi Minsup Kim Rohit Bhatia Naresh Kumar Ji Hoon Park Art. E. Cho Eun Ha Choi Weontae Lee
author_facet	Pankaj Attri Jitender Gaur Sooho Choi Minsup Kim Rohit Bhatia Naresh Kumar Ji Hoon Park Art. E. Cho Eun Ha Choi Weontae Lee
author_sort	Pankaj Attri
title	Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
title_short	Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
title_full	Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
title_fullStr	Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
title_full_unstemmed	Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
title_sort	interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1efb67bb46e745999fd5b85bddaf75eb
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_version_	1718388941550256128

Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein

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