Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)

Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure...

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Autores principales:	Bogunović Danica, Stević Nataša, Sidi-Boumedine Karim, Mišić Dušan, Tomanović Snežana, Kulišić Zoran, Magaš Vladimir, Radojičić Sonja
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	belgrade coxiella burnetii dogs ticks pcr Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/1efb7cc796f14d52834ebf2cb5ae6c2a
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spelling	oai:doaj.org-article:1efb7cc796f14d52834ebf2cb5ae6c2a2021-11-17T21:27:52ZMolecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)1820-744810.2478/acve-2018-0023https://doaj.org/article/1efb7cc796f14d52834ebf2cb5ae6c2a2018-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2018-0023https://doaj.org/toc/1820-7448Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecifi c transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confi rmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identifi ed: Rhipicephalus s anguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228) . The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.Bogunović DanicaStević NatašaSidi-Boumedine KarimMišić DušanTomanović SnežanaKulišić ZoranMagaš VladimirRadojičić SonjaSciendoarticlebelgradecoxiella burnetiidogstickspcrVeterinary medicineSF600-1100ENActa Veterinaria, Vol 68, Iss 3, Pp 257-268 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	belgrade coxiella burnetii dogs ticks pcr Veterinary medicine SF600-1100
spellingShingle	belgrade coxiella burnetii dogs ticks pcr Veterinary medicine SF600-1100 Bogunović Danica Stević Nataša Sidi-Boumedine Karim Mišić Dušan Tomanović Snežana Kulišić Zoran Magaš Vladimir Radojičić Sonja Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)
description	Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecifi c transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confi rmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identifi ed: Rhipicephalus s anguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228) . The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.
format	article
author	Bogunović Danica Stević Nataša Sidi-Boumedine Karim Mišić Dušan Tomanović Snežana Kulišić Zoran Magaš Vladimir Radojičić Sonja
author_facet	Bogunović Danica Stević Nataša Sidi-Boumedine Karim Mišić Dušan Tomanović Snežana Kulišić Zoran Magaš Vladimir Radojičić Sonja
author_sort	Bogunović Danica
title	Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)
title_short	Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)
title_full	Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)
title_fullStr	Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)
title_full_unstemmed	Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)
title_sort	molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in belgrade (serbia)
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/1efb7cc796f14d52834ebf2cb5ae6c2a
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Molecular Evidence of Q Fever Agent Coxiella Burnetii in Ixodid Ticks Collected from Stray Dogs in Belgrade (Serbia)

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