In vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use
Pierre-Antoine Deglesne,* Rodrigo Arroyo,* Javier Fidalgo López,* Lilian Sepúlveda,* Evgeniya Ranneva, Philippe Deprez Research and Development, Skin Tech Pharma Group, Castelló d’Empúries, Spain *These authors contributed equally to this work In...
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Dove Medical Press
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oai:doaj.org-article:1f0abac265234a41a5bec60b5154cb432021-12-02T07:00:10ZIn vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use1179-1470https://doaj.org/article/1f0abac265234a41a5bec60b5154cb432018-09-01T00:00:00Zhttps://www.dovepress.com/in-vitro-study-of-rrsreg-silisorg-ce-class-iii-medical-device-composed-peer-reviewed-article-MDERhttps://doaj.org/toc/1179-1470Pierre-Antoine Deglesne,* Rodrigo Arroyo,* Javier Fidalgo López,* Lilian Sepúlveda,* Evgeniya Ranneva, Philippe Deprez Research and Development, Skin Tech Pharma Group, Castelló d’Empúries, Spain *These authors contributed equally to this work Introduction: Silanol (organic silicon) has been used for decades in the treatment of skin photoaging as it stabilizes and maintains skin structures through hydrogen bonding electrostatic interaction with extracellular matrix (ECM) proteins or glycosaminoglycans. Organic silicon-based products are often presented as silanol derivatives which are currently associated to other structural molecules such as orthohydroxybenzoate, carboxymethyl theophylline alginate, ascorbate, acetyltyrosine, sodium lactate or mannuronate. Consequently, organic silicon formulations may differ substantially between the medical devices available on the market, which may result in additional effect on the skin. Therefore, there is a real need for a better characterization of the products in terms of their action on human skin and in vitro skin model. Materials and methods: In this in vitro study, the effect of RRS® Silisorg was analyzed. RRS® Silisorg is a dermal implant (CE Class III medical device) containing monomethylsilanol mannuronate associated to an antioxidant resveratrol. Skin fibroblast viability and capacity to induce the production of key ECM genes were evaluated in the presence of different concentrations of RRS® Silisorg. The key ECM genes selected were collagen type I, elastin and hyaluronan synthase type 2 (HAS2), which is the cellular enzyme responsible for high-molecular weight hyaluronic acid (HA) production. Viability was evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and expression was quantified by quantitative polymerase chain reaction. Results: RRS® Silisorg increased fibroblast gene expression of HAS2 in the first 24 hours, 25 times in the presence of 1 mg/mL of solution, followed by a collagen type I gene expression (4.7 times) and elastin expression (2.5 times) increase after 48 hours. Conclusion: These results demonstrate that the silanol-based medical device RRS® Silisorg sustains HA, collagen and elastin production in human skin fibroblasts in vitro. Keywords: dermal implant, mesotherapy, hyaluronic acid, collagen, organic silicon, photoagingDeglesne PAArroyo RFidalgo López JSepúlveda LRanneva EDeprez PDove Medical Pressarticledermal implantmesotherapyhyaluronic acidcollagenorganic siliconphotoaging.Medical technologyR855-855.5ENMedical Devices: Evidence and Research, Vol Volume 11, Pp 313-320 (2018) |
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dermal implant mesotherapy hyaluronic acid collagen organic silicon photoaging. Medical technology R855-855.5 |
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dermal implant mesotherapy hyaluronic acid collagen organic silicon photoaging. Medical technology R855-855.5 Deglesne PA Arroyo R Fidalgo López J Sepúlveda L Ranneva E Deprez P In vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use |
| description |
Pierre-Antoine Deglesne,* Rodrigo Arroyo,* Javier Fidalgo López,* Lilian Sepúlveda,* Evgeniya Ranneva, Philippe Deprez Research and Development, Skin Tech Pharma Group, Castelló d’Empúries, Spain *These authors contributed equally to this work Introduction: Silanol (organic silicon) has been used for decades in the treatment of skin photoaging as it stabilizes and maintains skin structures through hydrogen bonding electrostatic interaction with extracellular matrix (ECM) proteins or glycosaminoglycans. Organic silicon-based products are often presented as silanol derivatives which are currently associated to other structural molecules such as orthohydroxybenzoate, carboxymethyl theophylline alginate, ascorbate, acetyltyrosine, sodium lactate or mannuronate. Consequently, organic silicon formulations may differ substantially between the medical devices available on the market, which may result in additional effect on the skin. Therefore, there is a real need for a better characterization of the products in terms of their action on human skin and in vitro skin model. Materials and methods: In this in vitro study, the effect of RRS® Silisorg was analyzed. RRS® Silisorg is a dermal implant (CE Class III medical device) containing monomethylsilanol mannuronate associated to an antioxidant resveratrol. Skin fibroblast viability and capacity to induce the production of key ECM genes were evaluated in the presence of different concentrations of RRS® Silisorg. The key ECM genes selected were collagen type I, elastin and hyaluronan synthase type 2 (HAS2), which is the cellular enzyme responsible for high-molecular weight hyaluronic acid (HA) production. Viability was evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and expression was quantified by quantitative polymerase chain reaction. Results: RRS® Silisorg increased fibroblast gene expression of HAS2 in the first 24 hours, 25 times in the presence of 1 mg/mL of solution, followed by a collagen type I gene expression (4.7 times) and elastin expression (2.5 times) increase after 48 hours. Conclusion: These results demonstrate that the silanol-based medical device RRS® Silisorg sustains HA, collagen and elastin production in human skin fibroblasts in vitro. Keywords: dermal implant, mesotherapy, hyaluronic acid, collagen, organic silicon, photoaging |
| format |
article |
| author |
Deglesne PA Arroyo R Fidalgo López J Sepúlveda L Ranneva E Deprez P |
| author_facet |
Deglesne PA Arroyo R Fidalgo López J Sepúlveda L Ranneva E Deprez P |
| author_sort |
Deglesne PA |
| title |
In vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use |
| title_short |
In vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use |
| title_full |
In vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use |
| title_fullStr |
In vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use |
| title_full_unstemmed |
In vitro study of RRS® Silisorg CE Class III medical device composed of silanol: effect on human skin fibroblasts and its clinical use |
| title_sort |
in vitro study of rrs® silisorg ce class iii medical device composed of silanol: effect on human skin fibroblasts and its clinical use |
| publisher |
Dove Medical Press |
| publishDate |
2018 |
| url |
https://doaj.org/article/1f0abac265234a41a5bec60b5154cb43 |
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