Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin

Yung-Chih Kuo, Chien-Wei Tsao Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China Abstract: A drug delivery system of quercetin (QU)-encapsulated liposomes (LS) grafted with RMP-7, a bradykinin analog, and lactoferrin (Lf) was developed to permea...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kuo Y, Tsao C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Alzheimer’s disease blood–brain barrier RMP-7 lactoferrin quercetin liposome Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1f15891ecea848d4a9730b5e6c7c22d4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1f15891ecea848d4a9730b5e6c7c22d4
record_format	dspace
spelling	oai:doaj.org-article:1f15891ecea848d4a9730b5e6c7c22d42021-12-02T01:50:39ZNeuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin1178-2013https://doaj.org/article/1f15891ecea848d4a9730b5e6c7c22d42017-04-01T00:00:00Zhttps://www.dovepress.com/neuroprotection-against-apoptosis-of-sk-n-mc-cells-using-rmp-7--and-la-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yung-Chih Kuo, Chien-Wei Tsao Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China Abstract: A drug delivery system of quercetin (QU)-encapsulated liposomes (LS) grafted with RMP-7, a bradykinin analog, and lactoferrin (Lf) was developed to permeate the blood–brain barrier (BBB) and rescue degenerated neurons, acting as an Alzheimer’s disease (AD) pharmacotherapy. This colloidal formulation of QU-encapsulated LS grafted with RMP-7 and Lf (RMP-7-Lf-QU-LS) was used to traverse human brain microvascular endothelial cells (HBMECs) regulated by human astrocytes (HAs) and to treat SK-N-MC cells after an insult with cytotoxic β-amyloid (Aβ) fibrils. We found that surface RMP-7 and Lf enhanced the ability of QU to cross the BBB without inducing strong toxicity and damaging the tight junction. In addition, RMP-7-Lf-QU-LS significantly reduced Aβ-induced neurotoxicity and improved the viability of SK-N-MC cells. Compared with free QU, RMP-7-Lf-QU-LS could also significantly inhibit the expression of phosphorylated c-Jun N terminal kinase, phosphorylated p38, and phosphorylated tau protein at serine 202 by SK-N-MC cells, indicating an important role of RMP-7, Lf, and LS in protecting neurons against apoptosis. RMP-7-Lf-QU-LS is a promising carrier targeting the BBB to prevent Aβ-insulted neurodegeneration and may have potential in managing AD in future clinical applications. Keywords: Alzheimer’s disease, blood–brain barrier, β-amyloid, drug targeting, neurodegeneration, pharmacotherapyKuo YTsao CDove Medical PressarticleAlzheimer’s diseaseblood–brain barrierRMP-7lactoferrinquercetinliposomeMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2857-2869 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Alzheimer’s disease blood–brain barrier RMP-7 lactoferrin quercetin liposome Medicine (General) R5-920
spellingShingle	Alzheimer’s disease blood–brain barrier RMP-7 lactoferrin quercetin liposome Medicine (General) R5-920 Kuo Y Tsao C Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
description	Yung-Chih Kuo, Chien-Wei Tsao Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China Abstract: A drug delivery system of quercetin (QU)-encapsulated liposomes (LS) grafted with RMP-7, a bradykinin analog, and lactoferrin (Lf) was developed to permeate the blood–brain barrier (BBB) and rescue degenerated neurons, acting as an Alzheimer’s disease (AD) pharmacotherapy. This colloidal formulation of QU-encapsulated LS grafted with RMP-7 and Lf (RMP-7-Lf-QU-LS) was used to traverse human brain microvascular endothelial cells (HBMECs) regulated by human astrocytes (HAs) and to treat SK-N-MC cells after an insult with cytotoxic β-amyloid (Aβ) fibrils. We found that surface RMP-7 and Lf enhanced the ability of QU to cross the BBB without inducing strong toxicity and damaging the tight junction. In addition, RMP-7-Lf-QU-LS significantly reduced Aβ-induced neurotoxicity and improved the viability of SK-N-MC cells. Compared with free QU, RMP-7-Lf-QU-LS could also significantly inhibit the expression of phosphorylated c-Jun N terminal kinase, phosphorylated p38, and phosphorylated tau protein at serine 202 by SK-N-MC cells, indicating an important role of RMP-7, Lf, and LS in protecting neurons against apoptosis. RMP-7-Lf-QU-LS is a promising carrier targeting the BBB to prevent Aβ-insulted neurodegeneration and may have potential in managing AD in future clinical applications. Keywords: Alzheimer’s disease, blood–brain barrier, β-amyloid, drug targeting, neurodegeneration, pharmacotherapy
format	article
author	Kuo Y Tsao C
author_facet	Kuo Y Tsao C
author_sort	Kuo Y
title	Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
title_short	Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
title_full	Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
title_fullStr	Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
title_full_unstemmed	Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin
title_sort	neuroprotection against apoptosis of sk-n-mc cells using rmp-7- and lactoferrin-grafted liposomes carrying quercetin
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/1f15891ecea848d4a9730b5e6c7c22d4
work_keys_str_mv	AT kuoy neuroprotectionagainstapoptosisofsknmccellsusingrmp7andlactoferringraftedliposomescarryingquercetin AT tsaoc neuroprotectionagainstapoptosisofsknmccellsusingrmp7andlactoferringraftedliposomescarryingquercetin
_version_	1718402818359951360

Neuroprotection against apoptosis of SK-N-MC cells using RMP-7- and lactoferrin-grafted liposomes carrying quercetin

Ejemplares similares