Fructose-enhanced reduction of bacterial growth on nanorough surfaces

Naside Gozde Durmus1, Erik N Taylor1, Fatih Inci3,4, Kim M Kummer1, Keiko M Tarquinio5, Thomas J Webster1,21School of Engineering, Brown University, Providence, RI, USA; 2Department of Orthopedics, Brown University, Providence, RI, USA; 3Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bi...

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Autores principales:	Durmus NG, Taylor EN, Inci F, Kummer KM, Tarquinio KM, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1f1a0f5f161e47548d17bd7611d3550c
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spelling	oai:doaj.org-article:1f1a0f5f161e47548d17bd7611d3550c2021-12-02T03:05:06ZFructose-enhanced reduction of bacterial growth on nanorough surfaces1176-91141178-2013https://doaj.org/article/1f1a0f5f161e47548d17bd7611d3550c2012-02-01T00:00:00Zhttp://www.dovepress.com/fructose-enhanced-reduction-of-bacterial-growth-on-nanorough-surfaces-a9182https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Naside Gozde Durmus1, Erik N Taylor1, Fatih Inci3,4, Kim M Kummer1, Keiko M Tarquinio5, Thomas J Webster1,21School of Engineering, Brown University, Providence, RI, USA; 2Department of Orthopedics, Brown University, Providence, RI, USA; 3Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women&#39;s Hospital, Harvard-MIT Health Sciences and Technology, Harvard Medical School, MA, USA; 4Istanbul Technical University, Molecular Biology-Genetics and Biotechnology Program, Mobgam, Maslak, Istanbul, Turkey; 5Division of Pediatric Critical Care Medicine, Rhode Island Hospital, Providence, RI, USAAbstract: Patients on mechanical ventilators for extended periods of time often face the risk of developing ventilator-associated pneumonia. During the ventilation process, patients incapable of breathing are intubated with polyvinyl chloride (PVC) endotracheal tubes (ETTs). PVC ETTs provide surfaces where bacteria can attach and proliferate from the contaminated oropharyngeal space to the sterile bronchoalveolar area. To overcome this problem, ETTs can be coated with antimicrobial agents. However, such coatings may easily delaminate during use. Recently, it has been shown that changes in material topography at the nanometer level can provide antibacterial properties. In addition, some metabolites, such as fructose, have been found to increase the efficiency of antibiotics used to treat Staphylococcus aureus (S. aureus) infections. In this study, we combined the antibacterial effect of nanorough ETT topographies with sugar metabolites to decrease bacterial growth and biofilm formation on ETTs. We present for the first time that the presence of fructose on the nanorough surfaces decreases the number of planktonic S. aureus bacteria in the solution and biofilm formation on the surface after 24 hours. We thus envision that this method has the potential to impact the future of surface engineering of biomaterials leading to more successful clinical outcomes in terms of longer ETT lifetimes, minimized infections, and decreased antibiotic usage; all of which can decrease the presence of antibiotic resistant bacteria in the clinical setting.Keywords: antibacterial, medical device infection, ventilator-associated pneumonia, endotracheal tubes, nanoroughness, fructose, Staphylococcus aureusDurmus NGTaylor ENInci FKummer KMTarquinio KMWebster TJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 537-545 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Durmus NG Taylor EN Inci F Kummer KM Tarquinio KM Webster TJ Fructose-enhanced reduction of bacterial growth on nanorough surfaces
description	Naside Gozde Durmus1, Erik N Taylor1, Fatih Inci3,4, Kim M Kummer1, Keiko M Tarquinio5, Thomas J Webster1,21School of Engineering, Brown University, Providence, RI, USA; 2Department of Orthopedics, Brown University, Providence, RI, USA; 3Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women&#39;s Hospital, Harvard-MIT Health Sciences and Technology, Harvard Medical School, MA, USA; 4Istanbul Technical University, Molecular Biology-Genetics and Biotechnology Program, Mobgam, Maslak, Istanbul, Turkey; 5Division of Pediatric Critical Care Medicine, Rhode Island Hospital, Providence, RI, USAAbstract: Patients on mechanical ventilators for extended periods of time often face the risk of developing ventilator-associated pneumonia. During the ventilation process, patients incapable of breathing are intubated with polyvinyl chloride (PVC) endotracheal tubes (ETTs). PVC ETTs provide surfaces where bacteria can attach and proliferate from the contaminated oropharyngeal space to the sterile bronchoalveolar area. To overcome this problem, ETTs can be coated with antimicrobial agents. However, such coatings may easily delaminate during use. Recently, it has been shown that changes in material topography at the nanometer level can provide antibacterial properties. In addition, some metabolites, such as fructose, have been found to increase the efficiency of antibiotics used to treat Staphylococcus aureus (S. aureus) infections. In this study, we combined the antibacterial effect of nanorough ETT topographies with sugar metabolites to decrease bacterial growth and biofilm formation on ETTs. We present for the first time that the presence of fructose on the nanorough surfaces decreases the number of planktonic S. aureus bacteria in the solution and biofilm formation on the surface after 24 hours. We thus envision that this method has the potential to impact the future of surface engineering of biomaterials leading to more successful clinical outcomes in terms of longer ETT lifetimes, minimized infections, and decreased antibiotic usage; all of which can decrease the presence of antibiotic resistant bacteria in the clinical setting.Keywords: antibacterial, medical device infection, ventilator-associated pneumonia, endotracheal tubes, nanoroughness, fructose, Staphylococcus aureus
format	article
author	Durmus NG Taylor EN Inci F Kummer KM Tarquinio KM Webster TJ
author_facet	Durmus NG Taylor EN Inci F Kummer KM Tarquinio KM Webster TJ
author_sort	Durmus NG
title	Fructose-enhanced reduction of bacterial growth on nanorough surfaces
title_short	Fructose-enhanced reduction of bacterial growth on nanorough surfaces
title_full	Fructose-enhanced reduction of bacterial growth on nanorough surfaces
title_fullStr	Fructose-enhanced reduction of bacterial growth on nanorough surfaces
title_full_unstemmed	Fructose-enhanced reduction of bacterial growth on nanorough surfaces
title_sort	fructose-enhanced reduction of bacterial growth on nanorough surfaces
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/1f1a0f5f161e47548d17bd7611d3550c
work_keys_str_mv	AT durmusng fructoseenhancedreductionofbacterialgrowthonnanoroughsurfaces AT tayloren fructoseenhancedreductionofbacterialgrowthonnanoroughsurfaces AT incif fructoseenhancedreductionofbacterialgrowthonnanoroughsurfaces AT kummerkm fructoseenhancedreductionofbacterialgrowthonnanoroughsurfaces AT tarquiniokm fructoseenhancedreductionofbacterialgrowthonnanoroughsurfaces AT webstertj fructoseenhancedreductionofbacterialgrowthonnanoroughsurfaces
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Fructose-enhanced reduction of bacterial growth on nanorough surfaces

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