$Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis$
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Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis

Abstract Hevea brasiliensis Müll. Arg. is one of the most frequently wounded plants worldwide. Expelling latex upon mechanical injury is a wound response of rubber trees. However, JA-mediated wound responses in rubber trees are not well documented. In this work, three JAZ-interacting MYC transcripti...

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Autores principales:	Jinling Zhai, Hui Hao, Hua Xiao, Yuxin Cao, Xiangui Lin, Xi Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f1c518c6e614ba0a1e53806b311718b
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spelling	oai:doaj.org-article:1f1c518c6e614ba0a1e53806b311718b2021-12-02T15:09:05ZIdentification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis10.1038/s41598-018-19206-32045-2322https://doaj.org/article/1f1c518c6e614ba0a1e53806b311718b2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19206-3https://doaj.org/toc/2045-2322Abstract Hevea brasiliensis Müll. Arg. is one of the most frequently wounded plants worldwide. Expelling latex upon mechanical injury is a wound response of rubber trees. However, JA-mediated wound responses in rubber trees are not well documented. In this work, three JAZ-interacting MYC transcription factors of H. brasiliensis (termed HbMYC2/3/4) were identified by yeast two-hybrid screening. HbMYC2/3/4 each showed specific interaction profiles with HbJAZs. HbMYC2/3/4 each localized in the nucleus and exhibited strong transcriptional activity. To identify the target genes potentially regulated by HbMYC2/3/4, cis-elements interacting with HbMYC2/3/4 were first screened by yeast one-hybrid assays; the results indicated that HbMYC2/3/4 each could bind G-box elements. Additional analysis confirmed that HbMYC2/3/4 bound the HbPIP2;1 promoter, which contains five G-box cis-elements, and regulated the expression of reporter genes in yeast cells and in planta. HbMYC2/3/4 were induced by exogenous JA treatment but suppressed by ethylene (ET) treatment; in contrast, HbPIP2;1 was positively regulated by ET but negatively regulated by JA treatment. Given that HbPIP2;1 is involved in latex drainage, it could be proposed that HbMYC2/3/4 are involved in the regulation of HbPIP2;1 expression as well as latex drainage, both of which are coordinated by the JA and ET signalling pathways.Jinling ZhaiHui HaoHua XiaoYuxin CaoXiangui LinXi HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinling Zhai Hui Hao Hua Xiao Yuxin Cao Xiangui Lin Xi Huang Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
description	Abstract Hevea brasiliensis Müll. Arg. is one of the most frequently wounded plants worldwide. Expelling latex upon mechanical injury is a wound response of rubber trees. However, JA-mediated wound responses in rubber trees are not well documented. In this work, three JAZ-interacting MYC transcription factors of H. brasiliensis (termed HbMYC2/3/4) were identified by yeast two-hybrid screening. HbMYC2/3/4 each showed specific interaction profiles with HbJAZs. HbMYC2/3/4 each localized in the nucleus and exhibited strong transcriptional activity. To identify the target genes potentially regulated by HbMYC2/3/4, cis-elements interacting with HbMYC2/3/4 were first screened by yeast one-hybrid assays; the results indicated that HbMYC2/3/4 each could bind G-box elements. Additional analysis confirmed that HbMYC2/3/4 bound the HbPIP2;1 promoter, which contains five G-box cis-elements, and regulated the expression of reporter genes in yeast cells and in planta. HbMYC2/3/4 were induced by exogenous JA treatment but suppressed by ethylene (ET) treatment; in contrast, HbPIP2;1 was positively regulated by ET but negatively regulated by JA treatment. Given that HbPIP2;1 is involved in latex drainage, it could be proposed that HbMYC2/3/4 are involved in the regulation of HbPIP2;1 expression as well as latex drainage, both of which are coordinated by the JA and ET signalling pathways.
format	article
author	Jinling Zhai Hui Hao Hua Xiao Yuxin Cao Xiangui Lin Xi Huang
author_facet	Jinling Zhai Hui Hao Hua Xiao Yuxin Cao Xiangui Lin Xi Huang
author_sort	Jinling Zhai
title	Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
title_short	Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
title_full	Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
title_fullStr	Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
title_full_unstemmed	Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis
title_sort	identification of jaz-interacting myc transcription factors involved in latex drainage in hevea brasiliensis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1f1c518c6e614ba0a1e53806b311718b
work_keys_str_mv	AT jinlingzhai identificationofjazinteractingmyctranscriptionfactorsinvolvedinlatexdrainageinheveabrasiliensis AT huihao identificationofjazinteractingmyctranscriptionfactorsinvolvedinlatexdrainageinheveabrasiliensis AT huaxiao identificationofjazinteractingmyctranscriptionfactorsinvolvedinlatexdrainageinheveabrasiliensis AT yuxincao identificationofjazinteractingmyctranscriptionfactorsinvolvedinlatexdrainageinheveabrasiliensis AT xianguilin identificationofjazinteractingmyctranscriptionfactorsinvolvedinlatexdrainageinheveabrasiliensis AT xihuang identificationofjazinteractingmyctranscriptionfactorsinvolvedinlatexdrainageinheveabrasiliensis
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Identification of JAZ-interacting MYC transcription factors involved in latex drainage in Hevea brasiliensis

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