Micro-drive and headgear for chronic implant and recovery of optoelectronic probes

Abstract Silicon probes are multisite electrodes used for the electrophysiological recording of large neuronal ensembles. Optoelectronic probes (OEPs) are recent upgrades that allow, in parallel, the delivery of local optical stimuli. The procedures to use these delicate electrodes for chronic exper...

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Autores principales:	Jinho Chung, Farnaz Sharif, Dajung Jung, Soyoun Kim, Sebastien Royer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1f1f5eab46d442a98d3ee9c3f0bfd398
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spelling	oai:doaj.org-article:1f1f5eab46d442a98d3ee9c3f0bfd3982021-12-02T15:06:09ZMicro-drive and headgear for chronic implant and recovery of optoelectronic probes10.1038/s41598-017-03340-52045-2322https://doaj.org/article/1f1f5eab46d442a98d3ee9c3f0bfd3982017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03340-5https://doaj.org/toc/2045-2322Abstract Silicon probes are multisite electrodes used for the electrophysiological recording of large neuronal ensembles. Optoelectronic probes (OEPs) are recent upgrades that allow, in parallel, the delivery of local optical stimuli. The procedures to use these delicate electrodes for chronic experiments in mice are still underdeveloped and typically assume one-time uses. Here, we developed a micro-drive, a support for OEPs optical fibers, and a hat enclosure, which fabrications consist in fitting and fastening together plastic parts made with 3D printers. Excluding two parts, all components and electrodes are relatively simple to recover after the experiments, via the loosening of screws. To prevent the plugging of OEPs laser sources from altering the stability of recordings, the OEPs fibers can be transiently anchored to the hat via the tightening of screws. We test the stability of recordings in the mouse hippocampus under three different conditions: acute head-fixed, chronic head-fixed, and chronic freely moving. Drift in spike waveforms is significantly smaller in chronic compared to acute conditions, with the plugging/unplugging of head-stage and fiber connectors not affecting much the recording stability. Overall, these tools generate stable recordings of place cell in chronic conditions, and make the recovery and reuse of electrode packages relatively simple.Jinho ChungFarnaz SharifDajung JungSoyoun KimSebastien RoyerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinho Chung Farnaz Sharif Dajung Jung Soyoun Kim Sebastien Royer Micro-drive and headgear for chronic implant and recovery of optoelectronic probes
description	Abstract Silicon probes are multisite electrodes used for the electrophysiological recording of large neuronal ensembles. Optoelectronic probes (OEPs) are recent upgrades that allow, in parallel, the delivery of local optical stimuli. The procedures to use these delicate electrodes for chronic experiments in mice are still underdeveloped and typically assume one-time uses. Here, we developed a micro-drive, a support for OEPs optical fibers, and a hat enclosure, which fabrications consist in fitting and fastening together plastic parts made with 3D printers. Excluding two parts, all components and electrodes are relatively simple to recover after the experiments, via the loosening of screws. To prevent the plugging of OEPs laser sources from altering the stability of recordings, the OEPs fibers can be transiently anchored to the hat via the tightening of screws. We test the stability of recordings in the mouse hippocampus under three different conditions: acute head-fixed, chronic head-fixed, and chronic freely moving. Drift in spike waveforms is significantly smaller in chronic compared to acute conditions, with the plugging/unplugging of head-stage and fiber connectors not affecting much the recording stability. Overall, these tools generate stable recordings of place cell in chronic conditions, and make the recovery and reuse of electrode packages relatively simple.
format	article
author	Jinho Chung Farnaz Sharif Dajung Jung Soyoun Kim Sebastien Royer
author_facet	Jinho Chung Farnaz Sharif Dajung Jung Soyoun Kim Sebastien Royer
author_sort	Jinho Chung
title	Micro-drive and headgear for chronic implant and recovery of optoelectronic probes
title_short	Micro-drive and headgear for chronic implant and recovery of optoelectronic probes
title_full	Micro-drive and headgear for chronic implant and recovery of optoelectronic probes
title_fullStr	Micro-drive and headgear for chronic implant and recovery of optoelectronic probes
title_full_unstemmed	Micro-drive and headgear for chronic implant and recovery of optoelectronic probes
title_sort	micro-drive and headgear for chronic implant and recovery of optoelectronic probes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1f1f5eab46d442a98d3ee9c3f0bfd398
work_keys_str_mv	AT jinhochung microdriveandheadgearforchronicimplantandrecoveryofoptoelectronicprobes AT farnazsharif microdriveandheadgearforchronicimplantandrecoveryofoptoelectronicprobes AT dajungjung microdriveandheadgearforchronicimplantandrecoveryofoptoelectronicprobes AT soyounkim microdriveandheadgearforchronicimplantandrecoveryofoptoelectronicprobes AT sebastienroyer microdriveandheadgearforchronicimplantandrecoveryofoptoelectronicprobes
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Micro-drive and headgear for chronic implant and recovery of optoelectronic probes

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