Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury
Zearalenone(ZEA) is a kind of mycotoxin widely existing in nature, its toxic effects can lead to the reproductive disorders in humans and animals. The aim of this study was to investigate the mechanism of scutellarin against ovarian granulosa cell(GCs) injury induced by ZEA based on network pharmaco...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/1f2aab2fbfc64e75a5c88fd51d8b2901 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:1f2aab2fbfc64e75a5c88fd51d8b2901 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:1f2aab2fbfc64e75a5c88fd51d8b29012021-11-06T04:13:54ZNetwork pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury0147-651310.1016/j.ecoenv.2021.112865https://doaj.org/article/1f2aab2fbfc64e75a5c88fd51d8b29012021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321009775https://doaj.org/toc/0147-6513Zearalenone(ZEA) is a kind of mycotoxin widely existing in nature, its toxic effects can lead to the reproductive disorders in humans and animals. The aim of this study was to investigate the mechanism of scutellarin against ovarian granulosa cell(GCs) injury induced by ZEA based on network pharmacology, molecular docking method. The results show that 293 drug targets of scutellarin were found from PhamMapper database, and 583 disease targets were selected from Genecards database. Finally, 57 scutellarin targets were obtained for the repair of GCs injury with gene intersection. The protein-protein interaction(PPI), gene ontology(GO) and kyoto encyclopedia of genes and genomes(KEGG) analysis indicated that MAPK signaling pathway was most likely activated by scutellarin. Scutellarin with JNK or Caspase-3 had minimal and negative free binding energy in molecular docking analysis, indicating that they might be the acting targets of scutellarin. Cell viability was significantly decreased in ZEA treated cells. However, GCs viability, the level of estradiol(E2) and progesterone(P4) were significantly increased with addition of scutellarin to ZEA treated cells. Western blot analysis showed that scutellarin significantly reduced the expression of JNK, c-jun and Cleaved-caspasee-3 in GCs compared with ZEA treatment. In conclusion, scutellarin could alleviate the ovarian GCs injury by down-regulating the expression of JNK, c-jun and Cleaved-caspase-3 through the activation of MAPK/JNK signaling pathway. Our results will provide a theoretical foundation for the treatment of reproductive disorders with scutellarin.Panpan HuNa SunAjab khanXinyue ZhangPanpan SunYaogui SunJianzhong GuoXiaozhong ZhengWei YinKuohai FanJianzhong WangHuizhen YangHongquan LiElsevierarticleScutellarinZearalenoneGranulosa cell injuryNetwork pharmacologyMolecular dockingEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112865- (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Scutellarin Zearalenone Granulosa cell injury Network pharmacology Molecular docking Environmental pollution TD172-193.5 Environmental sciences GE1-350 |
| spellingShingle |
Scutellarin Zearalenone Granulosa cell injury Network pharmacology Molecular docking Environmental pollution TD172-193.5 Environmental sciences GE1-350 Panpan Hu Na Sun Ajab khan Xinyue Zhang Panpan Sun Yaogui Sun Jianzhong Guo Xiaozhong Zheng Wei Yin Kuohai Fan Jianzhong Wang Huizhen Yang Hongquan Li Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury |
| description |
Zearalenone(ZEA) is a kind of mycotoxin widely existing in nature, its toxic effects can lead to the reproductive disorders in humans and animals. The aim of this study was to investigate the mechanism of scutellarin against ovarian granulosa cell(GCs) injury induced by ZEA based on network pharmacology, molecular docking method. The results show that 293 drug targets of scutellarin were found from PhamMapper database, and 583 disease targets were selected from Genecards database. Finally, 57 scutellarin targets were obtained for the repair of GCs injury with gene intersection. The protein-protein interaction(PPI), gene ontology(GO) and kyoto encyclopedia of genes and genomes(KEGG) analysis indicated that MAPK signaling pathway was most likely activated by scutellarin. Scutellarin with JNK or Caspase-3 had minimal and negative free binding energy in molecular docking analysis, indicating that they might be the acting targets of scutellarin. Cell viability was significantly decreased in ZEA treated cells. However, GCs viability, the level of estradiol(E2) and progesterone(P4) were significantly increased with addition of scutellarin to ZEA treated cells. Western blot analysis showed that scutellarin significantly reduced the expression of JNK, c-jun and Cleaved-caspasee-3 in GCs compared with ZEA treatment. In conclusion, scutellarin could alleviate the ovarian GCs injury by down-regulating the expression of JNK, c-jun and Cleaved-caspase-3 through the activation of MAPK/JNK signaling pathway. Our results will provide a theoretical foundation for the treatment of reproductive disorders with scutellarin. |
| format |
article |
| author |
Panpan Hu Na Sun Ajab khan Xinyue Zhang Panpan Sun Yaogui Sun Jianzhong Guo Xiaozhong Zheng Wei Yin Kuohai Fan Jianzhong Wang Huizhen Yang Hongquan Li |
| author_facet |
Panpan Hu Na Sun Ajab khan Xinyue Zhang Panpan Sun Yaogui Sun Jianzhong Guo Xiaozhong Zheng Wei Yin Kuohai Fan Jianzhong Wang Huizhen Yang Hongquan Li |
| author_sort |
Panpan Hu |
| title |
Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury |
| title_short |
Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury |
| title_full |
Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury |
| title_fullStr |
Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury |
| title_full_unstemmed |
Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury |
| title_sort |
network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/1f2aab2fbfc64e75a5c88fd51d8b2901 |
| work_keys_str_mv |
AT panpanhu networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT nasun networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT ajabkhan networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT xinyuezhang networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT panpansun networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT yaoguisun networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT jianzhongguo networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT xiaozhongzheng networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT weiyin networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT kuohaifan networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT jianzhongwang networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT huizhenyang networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury AT hongquanli networkpharmacologybasedstudyonthemechanismofscutellarinagainstzearalenoneinducedovariangranulosacellinjury |
| _version_ |
1718443902952800256 |