Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature

The impacts of low temperature occasionally encountered at higher latitude regions on maize seed germination present significant threats to yield and cultivation. Exploring the association of antioxidant system with low temperature (LT) germination could support the breeding strategies for better re...

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Autores principales:	Senlin Xiao, Tianjun Xu, Yuandong Wang, Jinfeng Xing, Ronghuan Wang, Aiguo Su, Shuaishuai Wang, Wei Song, Jiuran Zhao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	maize low-temperature germination ascorbate Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/1f363bdb9cef4c80a541b3e8f797f62d
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spelling	oai:doaj.org-article:1f363bdb9cef4c80a541b3e8f797f62d2021-11-25T16:01:12ZCoordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature10.3390/agriculture111111602077-0472https://doaj.org/article/1f363bdb9cef4c80a541b3e8f797f62d2021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0472/11/11/1160https://doaj.org/toc/2077-0472The impacts of low temperature occasionally encountered at higher latitude regions on maize seed germination present significant threats to yield and cultivation. Exploring the association of antioxidant system with low temperature (LT) germination could support the breeding strategies for better responding to LT disturbance. In this study, we have examined the germination rate and growth potential of a set of elite maize inbred accessions under LT and normal temperature (NT) conditions in the field. These accessions were found to have variable germination rate and growth potential when grown at LT, whereas the difference is not significant under NT. Physiological study revealed lower hydrogen peroxide content in LT tolerant accessions when compared with sensitive ones. LT-tolerant and LT-sensitive lines maintained similar content of ascorbate (AsA) and glutathione (GSH), whereas the reduced substrate content of which were significantly higher in LT-tolerant accessions. Consistently, activities of ascorbate peroxidase and dehydroascorbate reductase, the enzyme components that responsible for the AsA-GSH recycling, were much higher in LT-tolerant lines. Transcription profile revealed the increased expression of <i>ZmVTC2</i> gene in LT-tolerant inbred line, which was rate limited step in AsA biosynthesis. These data indicates that the coordinated improvement of AsA biosynthesis and AsA-GSH recycling increase the pool size of the total antioxidants, which ameliorate LT-induced oxidative stress during maize seed germination.Senlin XiaoTianjun XuYuandong WangJinfeng XingRonghuan WangAiguo SuShuaishuai WangWei SongJiuran ZhaoMDPI AGarticlemaizelow-temperature germinationascorbateAgriculture (General)S1-972ENAgriculture, Vol 11, Iss 1160, p 1160 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	maize low-temperature germination ascorbate Agriculture (General) S1-972
spellingShingle	maize low-temperature germination ascorbate Agriculture (General) S1-972 Senlin Xiao Tianjun Xu Yuandong Wang Jinfeng Xing Ronghuan Wang Aiguo Su Shuaishuai Wang Wei Song Jiuran Zhao Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature
description	The impacts of low temperature occasionally encountered at higher latitude regions on maize seed germination present significant threats to yield and cultivation. Exploring the association of antioxidant system with low temperature (LT) germination could support the breeding strategies for better responding to LT disturbance. In this study, we have examined the germination rate and growth potential of a set of elite maize inbred accessions under LT and normal temperature (NT) conditions in the field. These accessions were found to have variable germination rate and growth potential when grown at LT, whereas the difference is not significant under NT. Physiological study revealed lower hydrogen peroxide content in LT tolerant accessions when compared with sensitive ones. LT-tolerant and LT-sensitive lines maintained similar content of ascorbate (AsA) and glutathione (GSH), whereas the reduced substrate content of which were significantly higher in LT-tolerant accessions. Consistently, activities of ascorbate peroxidase and dehydroascorbate reductase, the enzyme components that responsible for the AsA-GSH recycling, were much higher in LT-tolerant lines. Transcription profile revealed the increased expression of <i>ZmVTC2</i> gene in LT-tolerant inbred line, which was rate limited step in AsA biosynthesis. These data indicates that the coordinated improvement of AsA biosynthesis and AsA-GSH recycling increase the pool size of the total antioxidants, which ameliorate LT-induced oxidative stress during maize seed germination.
format	article
author	Senlin Xiao Tianjun Xu Yuandong Wang Jinfeng Xing Ronghuan Wang Aiguo Su Shuaishuai Wang Wei Song Jiuran Zhao
author_facet	Senlin Xiao Tianjun Xu Yuandong Wang Jinfeng Xing Ronghuan Wang Aiguo Su Shuaishuai Wang Wei Song Jiuran Zhao
author_sort	Senlin Xiao
title	Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature
title_short	Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature
title_full	Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature
title_fullStr	Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature
title_full_unstemmed	Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature
title_sort	coordinated effect of ascorbate biosynthesis and recycling in maize seed germination and seedling establishment under low temperature
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1f363bdb9cef4c80a541b3e8f797f62d
work_keys_str_mv	AT senlinxiao coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT tianjunxu coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT yuandongwang coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT jinfengxing coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT ronghuanwang coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT aiguosu coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT shuaishuaiwang coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT weisong coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature AT jiuranzhao coordinatedeffectofascorbatebiosynthesisandrecyclinginmaizeseedgerminationandseedlingestablishmentunderlowtemperature
_version_	1718413303769726976

Coordinated Effect of Ascorbate Biosynthesis and Recycling in Maize Seed Germination and Seedling Establishment under Low Temperature

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