Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release.
<h4>Background</h4>Biological control programs involving Wolbachia-infected Aedes aegypti are currently deployed in different epidemiological settings. New Caledonia (NC) is an ideal location for the implementation and evaluation of such a strategy as the only proven vector for dengue vi...
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2021
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oai:doaj.org-article:1f372d6cca084634bb7e8c591e59d8662021-12-02T20:24:10ZAssessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release.1935-27271935-273510.1371/journal.pntd.0009752https://doaj.org/article/1f372d6cca084634bb7e8c591e59d8662021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009752https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Biological control programs involving Wolbachia-infected Aedes aegypti are currently deployed in different epidemiological settings. New Caledonia (NC) is an ideal location for the implementation and evaluation of such a strategy as the only proven vector for dengue virus (DENV) is Ae. aegypti and dengue outbreaks frequency and severity are increasing. We report the generation of a NC Wolbachia-infected Ae. aegypti strain and the results of experiments to assess the vector competence and fitness of this strain for future implementation as a disease control strategy in Noumea, NC.<h4>Methods/principal findings</h4>The NC Wolbachia strain (NC-wMel) was obtained by backcrossing Australian AUS-wMel females with New Caledonian Wild-Type (NC-WT) males. Blocking of DENV, chikungunya (CHIKV), and Zika (ZIKV) viruses were evaluated via mosquito oral feeding experiments and intrathoracic DENV challenge. Significant reduction in infection rates were observed for NC-wMel Ae. aegypti compared to WT Ae. aegypti. No transmission was observed for NC-wMel Ae. aegypti. Maternal transmission, cytoplasmic incompatibility, fertility, fecundity, wing length, and insecticide resistance were also assessed in laboratory experiments. Ae. aegypti NC-wMel showed complete cytoplasmic incompatibility and a strong maternal transmission. Ae. aegypti NC-wMel fitness seemed to be reduced compared to NC-WT Ae. aegypti and AUS-wMel Ae. aegypti regarding fertility and fecundity. However further experiments are required to assess it accurately.<h4>Conclusions/significance</h4>Our results demonstrated that the NC-wMel Ae. aegypti strain is a strong inhibitor of DENV, CHIKV, and ZIKV infection and prevents transmission of infectious viral particles in mosquito saliva. Furthermore, our NC-wMel Ae. aegypti strain induces reproductive cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, supporting field-releases in Noumea, NC.Nicolas PocquetOlivia O'ConnorHeather A FloresJordan TutagataMorgane PolDavid J HookerCatherine InizanSylvie RussetJohanna M DuyvestynEtiene C PacidônioDominique GiraultDaniela da Silva GonçalvesMarine MinierFrédéric TouzainElodie ChalusKevin LucienFlorie CheilanTristan DeryckeSylvie LaumondCameron P SimmonsMyrielle Dupont-RouzeyrolNadège RossiPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 9, p e0009752 (2021) |
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EN |
| topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
| spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nicolas Pocquet Olivia O'Connor Heather A Flores Jordan Tutagata Morgane Pol David J Hooker Catherine Inizan Sylvie Russet Johanna M Duyvestyn Etiene C Pacidônio Dominique Girault Daniela da Silva Gonçalves Marine Minier Frédéric Touzain Elodie Chalus Kevin Lucien Florie Cheilan Tristan Derycke Sylvie Laumond Cameron P Simmons Myrielle Dupont-Rouzeyrol Nadège Rossi Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release. |
| description |
<h4>Background</h4>Biological control programs involving Wolbachia-infected Aedes aegypti are currently deployed in different epidemiological settings. New Caledonia (NC) is an ideal location for the implementation and evaluation of such a strategy as the only proven vector for dengue virus (DENV) is Ae. aegypti and dengue outbreaks frequency and severity are increasing. We report the generation of a NC Wolbachia-infected Ae. aegypti strain and the results of experiments to assess the vector competence and fitness of this strain for future implementation as a disease control strategy in Noumea, NC.<h4>Methods/principal findings</h4>The NC Wolbachia strain (NC-wMel) was obtained by backcrossing Australian AUS-wMel females with New Caledonian Wild-Type (NC-WT) males. Blocking of DENV, chikungunya (CHIKV), and Zika (ZIKV) viruses were evaluated via mosquito oral feeding experiments and intrathoracic DENV challenge. Significant reduction in infection rates were observed for NC-wMel Ae. aegypti compared to WT Ae. aegypti. No transmission was observed for NC-wMel Ae. aegypti. Maternal transmission, cytoplasmic incompatibility, fertility, fecundity, wing length, and insecticide resistance were also assessed in laboratory experiments. Ae. aegypti NC-wMel showed complete cytoplasmic incompatibility and a strong maternal transmission. Ae. aegypti NC-wMel fitness seemed to be reduced compared to NC-WT Ae. aegypti and AUS-wMel Ae. aegypti regarding fertility and fecundity. However further experiments are required to assess it accurately.<h4>Conclusions/significance</h4>Our results demonstrated that the NC-wMel Ae. aegypti strain is a strong inhibitor of DENV, CHIKV, and ZIKV infection and prevents transmission of infectious viral particles in mosquito saliva. Furthermore, our NC-wMel Ae. aegypti strain induces reproductive cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, supporting field-releases in Noumea, NC. |
| format |
article |
| author |
Nicolas Pocquet Olivia O'Connor Heather A Flores Jordan Tutagata Morgane Pol David J Hooker Catherine Inizan Sylvie Russet Johanna M Duyvestyn Etiene C Pacidônio Dominique Girault Daniela da Silva Gonçalves Marine Minier Frédéric Touzain Elodie Chalus Kevin Lucien Florie Cheilan Tristan Derycke Sylvie Laumond Cameron P Simmons Myrielle Dupont-Rouzeyrol Nadège Rossi |
| author_facet |
Nicolas Pocquet Olivia O'Connor Heather A Flores Jordan Tutagata Morgane Pol David J Hooker Catherine Inizan Sylvie Russet Johanna M Duyvestyn Etiene C Pacidônio Dominique Girault Daniela da Silva Gonçalves Marine Minier Frédéric Touzain Elodie Chalus Kevin Lucien Florie Cheilan Tristan Derycke Sylvie Laumond Cameron P Simmons Myrielle Dupont-Rouzeyrol Nadège Rossi |
| author_sort |
Nicolas Pocquet |
| title |
Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release. |
| title_short |
Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release. |
| title_full |
Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release. |
| title_fullStr |
Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release. |
| title_full_unstemmed |
Assessment of fitness and vector competence of a New Caledonia wMel Aedes aegypti strain before field-release. |
| title_sort |
assessment of fitness and vector competence of a new caledonia wmel aedes aegypti strain before field-release. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/1f372d6cca084634bb7e8c591e59d866 |
| work_keys_str_mv |
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