High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma

Pregnane X Receptor (PXR) is involved in human cancer, either by directly affecting carcinogenesis or by inducing drug-drug interactions and chemotherapy resistance. The clinical significance of PXR expression in invasive breast carcinoma was evaluated in the present study. PXR protein expression wa...

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Autores principales:	Stamatios Theocharis, Constantinos Giaginis, Stefania Gourzi, Paraskevi Alexandrou, Gerasimos Tsourouflis, Panagiotis Sarantis, Eugene Danas, Artemis Michail, Nikolaos Tsoukalas, Alexandros Pergaris, Panagiotis K. Politis, Lydia Nakopoulou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PXR breast cancer immunohistochemistry clinicopathological parameters patients’ prognosis cell lines Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/1f396469073443ceb893d4f78d997d61
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spelling	oai:doaj.org-article:1f396469073443ceb893d4f78d997d612021-11-25T17:20:08ZHigh Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma10.3390/diagnostics111119462075-4418https://doaj.org/article/1f396469073443ceb893d4f78d997d612021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4418/11/11/1946https://doaj.org/toc/2075-4418Pregnane X Receptor (PXR) is involved in human cancer, either by directly affecting carcinogenesis or by inducing drug-drug interactions and chemotherapy resistance. The clinical significance of PXR expression in invasive breast carcinoma was evaluated in the present study. PXR protein expression was assessed immunohistochemically on formalin fixed paraffin-embedded breast invasive carcinoma tissue sections, obtained from 148 patients, and was correlated with clinicopathological parameters, molecular phenotypes, tumor cells’ proliferative capacity, and overall disease-free patients’ survival. Additionally, the expression of PXR was examined on human breast carcinoma cell lines of different histological grade, hormonal status, and metastatic potential. PXR positivity was noted in 79 (53.4%) and high PXR expression in 48 (32.4%), out of 148 breast carcinoma cases. High PXR expression was positively associated with nuclear grade (<i>p</i> = 0.0112) and histological grade of differentiation (<i>p</i> = 0.0305), as well as with tumor cells’ proliferative capacity (<i>p</i> = 0.0051), and negatively with luminal A subtype (<i>p</i> = 0.0295). Associations between high PXR expression, estrogen, and progesterone receptor negative status were also recorded (<i>p</i> = 0.0314 and <i>p</i> = 0.0208, respectively). High PXR expression was associated with shorter overall patients’ survival times (log-rank test, <i>p</i> = 0.0009). In multivariate analysis, high PXR expression was identified as an independent prognostic factor of overall patients’ survival (Cox-regression analysis, <i>p</i> = 0.0082). PXR expression alterations were also noted in breast cancer cell lines of different hormonal status. The present data supported evidence that PXR was related to a more aggressive invasive breast carcinoma phenotype, being a strong and independent poor prognosticator.Stamatios TheocharisConstantinos GiaginisStefania GourziParaskevi AlexandrouGerasimos TsourouflisPanagiotis SarantisEugene DanasArtemis MichailNikolaos TsoukalasAlexandros PergarisPanagiotis K. PolitisLydia NakopoulouMDPI AGarticlePXRbreast cancerimmunohistochemistryclinicopathological parameterspatients’ prognosiscell linesMedicine (General)R5-920ENDiagnostics, Vol 11, Iss 1946, p 1946 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PXR breast cancer immunohistochemistry clinicopathological parameters patients’ prognosis cell lines Medicine (General) R5-920
spellingShingle	PXR breast cancer immunohistochemistry clinicopathological parameters patients’ prognosis cell lines Medicine (General) R5-920 Stamatios Theocharis Constantinos Giaginis Stefania Gourzi Paraskevi Alexandrou Gerasimos Tsourouflis Panagiotis Sarantis Eugene Danas Artemis Michail Nikolaos Tsoukalas Alexandros Pergaris Panagiotis K. Politis Lydia Nakopoulou High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma
description	Pregnane X Receptor (PXR) is involved in human cancer, either by directly affecting carcinogenesis or by inducing drug-drug interactions and chemotherapy resistance. The clinical significance of PXR expression in invasive breast carcinoma was evaluated in the present study. PXR protein expression was assessed immunohistochemically on formalin fixed paraffin-embedded breast invasive carcinoma tissue sections, obtained from 148 patients, and was correlated with clinicopathological parameters, molecular phenotypes, tumor cells’ proliferative capacity, and overall disease-free patients’ survival. Additionally, the expression of PXR was examined on human breast carcinoma cell lines of different histological grade, hormonal status, and metastatic potential. PXR positivity was noted in 79 (53.4%) and high PXR expression in 48 (32.4%), out of 148 breast carcinoma cases. High PXR expression was positively associated with nuclear grade (<i>p</i> = 0.0112) and histological grade of differentiation (<i>p</i> = 0.0305), as well as with tumor cells’ proliferative capacity (<i>p</i> = 0.0051), and negatively with luminal A subtype (<i>p</i> = 0.0295). Associations between high PXR expression, estrogen, and progesterone receptor negative status were also recorded (<i>p</i> = 0.0314 and <i>p</i> = 0.0208, respectively). High PXR expression was associated with shorter overall patients’ survival times (log-rank test, <i>p</i> = 0.0009). In multivariate analysis, high PXR expression was identified as an independent prognostic factor of overall patients’ survival (Cox-regression analysis, <i>p</i> = 0.0082). PXR expression alterations were also noted in breast cancer cell lines of different hormonal status. The present data supported evidence that PXR was related to a more aggressive invasive breast carcinoma phenotype, being a strong and independent poor prognosticator.
format	article
author	Stamatios Theocharis Constantinos Giaginis Stefania Gourzi Paraskevi Alexandrou Gerasimos Tsourouflis Panagiotis Sarantis Eugene Danas Artemis Michail Nikolaos Tsoukalas Alexandros Pergaris Panagiotis K. Politis Lydia Nakopoulou
author_facet	Stamatios Theocharis Constantinos Giaginis Stefania Gourzi Paraskevi Alexandrou Gerasimos Tsourouflis Panagiotis Sarantis Eugene Danas Artemis Michail Nikolaos Tsoukalas Alexandros Pergaris Panagiotis K. Politis Lydia Nakopoulou
author_sort	Stamatios Theocharis
title	High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma
title_short	High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma
title_full	High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma
title_fullStr	High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma
title_full_unstemmed	High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma
title_sort	high pregnane x receptor (pxr) expression is correlated with poor prognosis in invasive breast carcinoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/1f396469073443ceb893d4f78d997d61
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High Pregnane X Receptor (PXR) Expression Is Correlated with Poor Prognosis in Invasive Breast Carcinoma

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